Review of: Richard 0. Gamble II, How to Reduce Professional Liability for Engineers and Architects

Review of Richard 0. Gamble II, How to Reduce Professional Liability for Engineers and Architects (Noyes Data Corporation 1987) Foreword, references, index, table of cases. LC: 87-12256; ISBN: 0-8155-1128-0. [102 pp. Cloth $36.00. Mill Road, Park Ridge NJ 07656.

GENOMIC AND PHYSIOLOGICAL BIOREMEDIATION CHARACTERISTICS OF THE ACTINOBACTERIA FRANKIA

Introduction: Frankia is a genus of symbiotic, nitrogen fixing actinobacteria found in the soil and root tissue of actinorhizal plants throughout the world. Both Frankia and actinorhizal plants are resistant to a wide range of environmental and chemical stresses including heavy metals, high salinity, and drought. This makes Frankia and the actinorhizal symbiosis an interesting candidate for bioremediation applications. However, the microbial community found in the actinorhizal rhizosphere and surrounding soil has been implicated in some of the symbiotic and resistance traits attributed to Frankia. This necessitates thorough investigation of the genomic and genetic components of any bioremediation traits possessed by Frankia. Aims: This work pursued three primary aims: 1). To expand the range of available whole genome sequences available for the genus Frankia and use them to assess the pan-genome characteristics of Frankia, 2). To determine the composition of the microbial communities associated with Frankia and actinorhizal plants, and 3). To assess the potential of Frankia to remediate dioxin-like compounds. Results: Pan-genome analysis of 45 Frankia genomes revealed that the Frankia genus has small core genome made up of 44 genes with 9013 shell genes and over 62,000 cloud genes. Analysis also showed that Frankia has an open pan-genome structure with a Heap’s alpha value of 0.86. Analysis of the 5 clades that form the Frankia genus indicated that each clade has a closed pan-genome structure with core genome sizes ranging from 383 to 3414. Metagenomic analysis of the microbiomes associated with Coriaria myrtifolia, Casuarina glauca, and New Hampshire lithic environments identified highly diverse communities that included Frankia strains not previously known to be associated with these specific actinorhizal hosts. NMDS analysis showed that each tissue type assessed had a unique microbial community structure. This work also found that microbial diversity was positively correlated with proximity to an actinorhizal host plant. Bioinformatic analysis identified 5 Frankia strains with a putative bph operon. Two strains were assessed further and exhibited resistance to dioxin-like compounds (biphenyl, 4-chlorobiphenyl, and dibenzofuran). The same two strains, EuI1c and EUN1f, were able to grow with dioxin-like compounds as a sole source of carbon in minimal media. RNA sequencing and qPCR showed that the putative bph operon was up-regulated in Frankia in response to dioxin-like compound exposure. Lastly, GC-FID analysis showed that Frankia is able to degrade biphenyl in liquid culture. These results advance the basic understanding of Frankia biology, and show that Frankia has the ability to be used as a bioremediation agent

GENOMIC AND PHYSIOLOGICAL BIOREMEDIATION CHARACTERISTICS OF THE ACTINOBACTERIA FRANKIA

Introduction: Frankia is a genus of symbiotic, nitrogen fixing actinobacteria found in the soil and root tissue of actinorhizal plants throughout the world. Both Frankia and actinorhizal plants are resistant to a wide range of environmental and chemical stresses including heavy metals, high salinity, and drought. This makes Frankia and the actinorhizal symbiosis an interesting candidate for bioremediation applications. However, the microbial community found in the actinorhizal rhizosphere and surrounding soil has been implicated in some of the symbiotic and resistance traits attributed to Frankia. This necessitates thorough investigation of the genomic and genetic components of any bioremediation traits possessed by Frankia. Aims: This work pursued three primary aims: 1). To expand the range of available whole genome sequences available for the genus Frankia and use them to assess the pan-genome characteristics of Frankia, 2). To determine the composition of the microbial communities associated with Frankia and actinorhizal plants, and 3). To assess the potential of Frankia to remediate dioxin-like compounds. Results: Pan-genome analysis of 45 Frankia genomes revealed that the Frankia genus has small core genome made up of 44 genes with 9013 shell genes and over 62,000 cloud genes. Analysis also showed that Frankia has an open pan-genome structure with a Heap’s alpha value of 0.86. Analysis of the 5 clades that form the Frankia genus indicated that each clade has a closed pan-genome structure with core genome sizes ranging from 383 to 3414. Metagenomic analysis of the microbiomes associated with Coriaria myrtifolia, Casuarina glauca, and New Hampshire lithic environments identified highly diverse communities that included Frankia strains not previously known to be associated with these specific actinorhizal hosts. NMDS analysis showed that each tissue type assessed had a unique microbial community structure. This work also found that microbial diversity was positively correlated with proximity to an actinorhizal host plant. Bioinformatic analysis identified 5 Frankia strains with a putative bph operon. Two strains were assessed further and exhibited resistance to dioxin-like compounds (biphenyl, 4-chlorobiphenyl, and dibenzofuran). The same two strains, EuI1c and EUN1f, were able to grow with dioxin-like compounds as a sole source of carbon in minimal media. RNA sequencing and qPCR showed that the putative bph operon was up-regulated in Frankia in response to dioxin-like compound exposure. Lastly, GC-FID analysis showed that Frankia is able to degrade biphenyl in liquid culture. These results advance the basic understanding of Frankia biology, and show that Frankia has the ability to be used as a bioremediation agent

Session A-4: Makerspaces in the School: Understanding and Creating an Educational Makerspace

This session will cover the process of opening and running a school-based Makerspace, using our Makerspace’s journey as the lens. The learning outcomes for this seminar will be as follows: understanding what a Makerspace is, and how it fits into the school environment; figuring out what equipment you need; how to tailor your space to your situation; and what it takes to run and upkeep a Makerspace. Additionally we will tour IMSA’s IN2 Makerspace, and end with a Q&A session to address any specific inquiries

An analysis of prosocial behavior in customers

Despite all that is known about the good entrepreneurship can do for communities, there is still a gap in the research on how entrepreneurship can directly better communities and the people that live in them through passing on prosocial benefits. This paper proposes “prosocial entrepreneurship” as a means to address this gap. It will address the gap through examining how prosocial entrepreneurship builds communities to pass on a positive impact on these communities. Businesses have both encouraged prosocial behaviors in employees and promoted cooperation and community building for some time. Research was also completed on prosocial behaviors to build a better understanding of prosocial entrepreneurship. The prosocial entrepreneur sets itself apart from its predecessors through the focus on instilling their values on customers served to improve them. Theoretical Propositions on the effects of prosocial entrepreneurship have been suggested, and research on existing companies using these theoretical propositions, such as Jeep, has been presented. The research concludes with an application of it in the form of a sample business model that utilizes the aspects examined previously in the paper

Partitions/ apparitions

textThis Masters Report discusses my pivotal works from the past three years, as well as the generative concepts and stories behind them. Ubiquitous forms and anachronistic processes, pushed to the periphery for sheer abundance or seeming obsolescence, are two of my points of focus.Studio Ar

Graduate Recital:Erik Swanson, Guitar

Kemp Recital Hall Friday Evening April 29, 2005 6:30p.m

Charles W. Bolen Faculty Recital Series: Erik Swanson, Guitar; March 27, 2022

Kemp Recital Hall March 27, 2022 Sunday Afternoon 2:00 p.m

BCFT in a Black Hole Background: An Analytical Holographic Model

We study the entanglement phase structure of a holographic boundary conformal field theory (BCFT) in a two-dimensional black hole background. The bulk dual is the AdS$_3$ black string geometry with a Karch-Randall brane. We compute the subregion entanglement entropy of various two-sided bipartitions to elucidate the phase space where a Page curve exists in this setup. We do fully analytical computations on both the gravity side and the field theory side and demonstrate that the results precisely match. We discuss the entanglement phase structure describing where a Page curve exists in this geometry in the context of these analytical results. This is a useful model to study entanglement entropy for quantum field theory on a curved background.Comment: 30 pages, 11 figures, comments and references are welcome