Impacts of alternative grassland management regimes on the population ecology of grassland birds

We investigated the impacts of rangeland management practices on the diversity, density, and nest survival of grassland songbirds and on the demography, habitat selection, and population viability of Greater Prairie-Chickens (Tympanuchus cupido). Our study site was located in eastcentral Kansas and included portions of Chase, Greenwood, Lyon, and Morris Counties. We had access to ~1,100 km2 of private and public lands. Over 95% of the land was privately owned (~1040 km2), and the remaining ~5% was the Tallgrass Prairie National Preserve. Our final project report is based on data collected during a 3-year period from Feb. 2011 through Feb. 2014. Our study focused on grassland songbirds and Greater Prairie-Chickens as indicators of ecosystem response. Our analyses are based on direct comparisons between monitoring efforts in pastures managed with patch-burn grazing (PBG) and pastures managed with traditional intensive early stocking and annual burning (IESB).Citation: Sandercock, B.K., V.L. Winder, A.E. Erickson, and L.B. McNew. 2014. Impacts of alternative grassland management regimes on the population ecology of grassland birds. Kansas Department of Wildlife, Parks and Tourism, Final Project Report for Award No. KDWP-W-67-R. (technical report)

Effects of Lime Amendment on Antibiotic Resistance in Beef Cattle Manure of Open Feedlots

The objective of this study was to evaluate the effectiveness of lime amendment on the reduction of antimicrobial resistant bacteria and antimicrobial resistance genes in beef cattle manure in open feedlots. Hydrated lime was uniformly applied to the surface of feedlot pen floor 1 day prior to cattle harvest at a rate of 0.36 lb/ft2 and samples were collected over time. Collected samples were analyzed for change in pH and levels of antimicrobial resistant bacteria and antimicrobial resistance genes. Lime amendment elevated the pH of pen floor surface materials to pH \u3e 12 for 4 hours and then pH \u3e 11 for another 24 hours. Lime amendment reduced the concentration of generic and resistant Escherichia coli by 1–2 log for up to 4 hours. The abundance of antimicrobial resistance genes, such as tet(X) and tet(O), decreased by 1–2 order of magnitude with lime amendment. Results indicate that lime addition reduced the concentrations of antimicrobial resistant bacteria and antimicrobial resistance genes in pen surface materials from open beef feedlot pens

Effect of Feeding Different Types of Byproducts and Concentrations Throughout a Beef Growing System on Ground Beef Color and Lipid Oxidation

The objective of this trial was to evaluate the effect of feeding different concentrations of wet distillers grains during winter backgrounding and either modified wet distillers grains or Sweet Bran® during the finishing phase on ground beef color and lipid oxidation. After a 14 day aging period, ground beef patties were made and placed in a simulated retail display for seven days. There were no overall differences in lipid oxidation between treatments but was a treatment by day interaction for discoloration. Ground beef from heifers finished with modified wet distillers grains discolored at a greater extent when compared to ground beef from heifers finished with Sweet Bran

Descriptive analysis of seizures and comorbidities associated with fragile X syndrome

Background Fragile X syndrome is characterized by a myriad of physical features, behavioral features, and medical problems. Commonly found behavioral features are hyperactivity, anxiety, socialization difficulties, and ASD. There is also a higher incidence than in the general population of strabismus, otitis media, and mitral valve prolapse. In addition, one of the most common medical problems associated with FXS is an increased risk of seizures. A subset of individuals carrying the full mutation of the FMR1 gene and diagnosed with fragile X syndrome (FXS) are reported to experience seizures, mostly during the first 10 years of their life span. Methods As part of a larger project to identify genetic variants that modify the risk of seizures, we collected clinical information from 49 carriers with FXS who experienced seizures and 46 without seizures. We compared seizure type and comorbid conditions based on the source of data as well as family history of seizures. Results We found that the concordance of seizure types observed by parents and medical specialists varied by type of seizure. The most common comorbid condition among those with seizures was autism spectrum disorder (47% per medical records vs. 33% per parent report compared with 19% among those without seizures per parent report); the frequency of other comorbid conditions did not differ among groups. We found a slightly higher frequency of family members who experienced seizures among the seizure group compared with the nonseizure group. Conclusion This study confirms previously reported features of seizures in FXS, supports additional genetic factors, and highlights the importance of information sources, altogether contributing to a better understanding of seizures in FXS

An Analysis of Private School Closings

We add to the small literature on private school supply by exploring exits of K-12 private schools. We find that the closure of private schools is not an infrequent event, and use national survey data from the National Center for Education Statistics to study closures of private schools. We assume that the probability of an exit is a function of excess supply of private schools over the demand, as well as the school's characteristics such as age, size, and religious affiliation. Our empirical results generally support the implications of the model. Working Paper 07-0

No Difference in Arousal or Cognitive Demands Between Manual and Partially Automated Driving: A Multi-Method On-Road Study

IntroductionPartial driving automation is not always reliable and requires that drivers maintain readiness to take over control and manually operate the vehicle. Little is known about differences in drivers’ arousal and cognitive demands under partial automation and how it may make it difficult for drivers to transition from automated to manual modes. This research examined whether there are differences in drivers’ arousal and cognitive demands during manual versus partial automation driving.MethodWe compared arousal (using heart rate) and cognitive demands (using the root mean square of successive differences in normal heartbeats; RMSSD, and Detection Response Task; DRT) while 39 younger (M = 28.82 years) and 32 late-middle-aged (M = 52.72 years) participants drove four partially automated vehicles (Cadillac, Nissan Rogue, Tesla, and Volvo) on interstate highways. If compared to manual driving, drivers’ arousal and cognitive demands were different under partial automation, then corresponding differences in heart rate, RMSSD, and DRT would be expected. Alternatively, if drivers’ arousal and cognitive demands were similar in manual and partially automated driving, no difference in the two driving modes would be expected.ResultsResults suggest no significant differences in heart rate, RMSSD, or DRT reaction time performance between manual and partially automated modes of driving for either younger or late-middle-aged adults across the four test vehicles. A Bayes Factor analysis suggested that heart rate, RMSSD, and DRT data showed extreme evidence in favor of the null hypothesis.ConclusionThis novel study conducted on real roads with a representative sample provides important evidence of no difference in arousal and cognitive demands. Younger and late-middle-aged motorists who are new to partial automation are able to maintain arousal and cognitive demands comparable to manual driving while using the partially automated technology. Drivers who are more experienced with partially automated technology may respond differently than those with limited prior experience

Preclinical Activity of Eltrombopag (SB-497115), an Oral, Nonpeptide Thrombopoietin Receptor Agonist

Eltrombopag is a first-in-class, orally bioavailable, small-molecule, nonpeptide agonist of the thrombopoietin receptor (TpoR), which is being developed as a treatment for thrombocytopenia of various etiologies. In vitro studies have demonstrated that the activity of eltrombopag is dependent on expression of TpoR, which activates the signaling transducers and activators of transcription (STAT) and mitogen-activated protein kinase signal transduction pathways. The objective of this preclinical study is to determine if eltrombopag interacts selectively with the TpoR to facilitate megakaryocyte differentiation in platelets. Functional thrombopoietic activity was demonstrated by the proliferation and differentiation of primary human CD34+ bone marrow cells into CD41+ megakaryocytes. Measurements in platelets in several species indicated that eltrombopag specifically activates only the human and chimpanzee STAT pathways. The in vivo activity of eltrombopag was demonstrated by an increase of up to 100% in platelet numbers when administered orally (10 mg/kg per day for 5 days) to chimpanzees. In conclusion, eltrombopag interacts selectively with the TpoR without competing with Tpo, leading to the increased proliferation and differentiation of human bone marrow progenitor cells into megakaryocytes and increased platelet production. These results suggest that eltrombopag and Tpo may be able to act additively to increase platelet production

A Dynamic Pathway for Calcium-Independent Activation of CaMKII by Methionine Oxidation

SummaryCalcium/calmodulin (Ca2+/CaM)-dependent protein kinase II (CaMKII) couples increases in cellular Ca2+ to fundamental responses in excitable cells. CaMKII was identified over 20 years ago by activation dependence on Ca2+/CaM, but recent evidence shows that CaMKII activity is also enhanced by pro-oxidant conditions. Here we show that oxidation of paired regulatory domain methionine residues sustains CaMKII activity in the absence of Ca2+/CaM. CaMKII is activated by angiotensin II (AngII)-induced oxidation, leading to apoptosis in cardiomyocytes both in vitro and in vivo. CaMKII oxidation is reversed by methionine sulfoxide reductase A (MsrA), and MsrA−/− mice show exaggerated CaMKII oxidation and myocardial apoptosis, impaired cardiac function, and increased mortality after myocardial infarction. Our data demonstrate a dynamic mechanism for CaMKII activation by oxidation and highlight the critical importance of oxidation-dependent CaMKII activation to AngII and ischemic myocardial apoptosis

Overweight, Obesity and Underweight Is Associated with Adverse Psychosocial and Physical Health Outcomes among 7-Year-Old Children: The 'Be Active, Eat Right' Study

Background:Limited studies have reported on associations between overweight, and physical and psychosocial health outcomes among younger children. This study evaluates associations between overweight, obesity and underweight in 5-year-old children, and parent-reported health outcomes at age 7 years.Methods:Data were used from the 'Be active, eat right' study. Height and weight were measured at 5 and 7 years. Parents reported on child physical and psychosocial health outcomes (e.g. respiratory symptoms, general health, happiness, insecurity and adverse treatment). Regression models, adjusted for potential confounders, were fitted to predict health outcomes at age 7 years.Results:The baseline study sample consisted of 2,372 children mean age 5.8 (SD 0.4) years; 6.2% overweight, 1.6% obese and 15.0% underweight. Based on parent-report, overweight, obese and underweight children had an odds ratio (OR) of 5.70 (95% CI: 4.10 to 7.92), 35.34 (95% CI: 19.16; 65.17) and 1.39 (95% CI: 1.05 to 1.84), respectively, for being treated adversely compared to normal weight children. Compared to children with a low stable body mass index (BMI), parents of children with a high stable BMI reported their child to have an OR of 3.87 (95% CI: 1.75 to 8.54) for visiting the general practitioner once or more, an OR of 15.94 (95% CI: 10.75 to 23.64) for being treated adversely, and an OR of 16.35 (95% CI: 11.08 to 24.36) for feeling insecure.Conclusion:This study shows that overweight, obesity and underweight at 5 years of age is associated with more parent-reported adverse treatment of the child. Qualitative research examining underlying mechanisms is recommended. Healthcare providers should be aware of the possible adverse effects of childhood overweight and also relative underweight, and provide parents and children with appropriate counseling