Histomorphometrical study of silver carp fish testis in two age classes

In this research, morphological and histomorphometrical structure of testis of 20 silver male carp fish were studied in two classes or groups. Group1 was composed of 10 fish with average (±SD) weight of 1.247+0.656kg and average(±SD) length of 43.675+1.414cm with about 2 years age, Group2 was composed of 10 fish with average(±SD) weight of 5.716+0.519kg and average(±SD) length of 81.5+1.643cm. Average (±SD) weight of testis were 2.34+1.47gr and 83.33+25.81gr with average (±SD) GSI of 0.187+0.224 and 1.457+4.974 in groups 1 and 2 respectively. Samples from testis were taken by maximum thickness of 0.5cm and after fixation in bouin , s fixative and 5-6µm thickness section were made routine paraffin embedding method and stained by Hematoxylin-Eosin and PAS staining. The microscopic results showed that the silver carp testis was lobular and cystic type in two groups. In group 1, there was no spermatozoon activity and PGCs were only germ cells in the cysts. But in group2, the numbers of PGCs were decreased significantly and spermatogenic cells were seen in different phases including spermatogonia, primary and secondary spermatocysts, early and late spermatid, and spermatozoa which each one was located in a separated cyst. There was no significant difference in nucleus diameter of PGCs in testis of group1 (6.97+0.438µ) and group (6.13+0.438µ). In group2, the nucleolus diameter of spermatogonia was 2.97+0.112µm, primary spermatocyt 3.59+0.107µ, early spermatid 1.59+0.761µ, late spermatid 1.24+0.132µ, spermatozoa 1.16+0.054µ, and the length of spermatozoia 17.412+1.946µ. The interesting finding was immature testis in fish of group 1 with average weigh (1.247+0.656kg) and average length (43.675+1.414cm) in about 2 years age and mature testis in fish of group 2 with average weight of (5.716+0.519kg) and average length of (81.5+1.643cm) with about 4 years age in Khuzestan climate conditions

Isolation of common carp ovarian follicular cells and evaluation of their endocrine activity in primary cell culture

To study viability and activity of isolated common carp, Cyprinus carpio, ovarian follicular cells (granulosa and theca cells), 17-α-Hydroxy progesterone (17α-OHP) and 17β-Estradiol (E2) levels were estimated in the culture media of cultivated carp ovarian follicular cells, using radioimmunoassay (RIA). Oocytes were isolated from the ovaries of female carp. Interstitial tissue was manually removed in order to obtain single oocytes surrounded only by the follicular envelope. Such a preparation was trypsinized at room temperature. Follicular cell suspension contained both cells and cell clumps. It was the mixture of theca (T) and granulosa (G) cells. The cells were grown as monolayer in 24-well microplates in M199 medium supplemented with FBS. Culture media were analyzed for estrogen and progesterone content by appropriate radioimmunoassay. Trypsinization of ovarian follicles resulted in the formation of the cell suspension which contained a mixture of G and T cells. The follicular cells attached to glass and grew well during culture period. E2 was the main steroid hormone secreted by cultivated cells. Estrogen secretion increased by 415.52 ± 25 pg/ml at the first 3 days up to 530.25 ± 55.8 pg/ml on day 5 and it didn't change significantly until the end of the experiment. 17α-OHP secretion, however, gradually increased from 23.84 ± 8.2 pg/ml at the beginning of culture up to 35.76 ± 5.4 pg/ml at the end of cultivating. As the result of the present study the fish follicular cells grown in tissue culture were steroidogenically active cells as expressed by the secretion of E2 and P4 and the E2 was a dominant hormone secreted by isolated follicular cells, which it correlated closely with vitellogenes stage

Effect of Carbohydrate Source and Addition Canola Seed on Growth Performance and Nutrient Digestibility of Lambs

The objective of this study was to investigate effects of partial replacement of starch with neutral detergent soluble fiber with or without roasted canola seed as a fat source, and possible interactions on apparent digestibility and growth performance of growing lambs. For this purpose, 24 male lambs were used in a completely randomized design with a 2×2 factorial arrangement of treatments. Dietary treatments were 1- starch from barley, 2- starch from barley with roasted canola seed, 3- soluble fiber from beet pulp, and 4- soluble fiber from beet pulp with roasted canola seed. The experimental period was 84 days and forage:concentrate ratio was 10:90 for all diets. Treatments had no significant effect on the dry matter intake, feed conversion ratio, apparent digestibility of protein and organic matter (p>0.05). High starch diets compared with high soluble fiber diets caused a significant decrease in the digestibility of dry matter, organic matter, ADF and NDF (p<0.01). Canola seed addition to the diets significantly decreased ADF and NDF digestibility (p<0.01). The carbohydrate source by canola seed interaction was significant for weight gain and rumen pH (p<0.01) indicating that addition of canola seed to high starch diet increased weight gain and rumen pH more dramatically in comparison with soluble fiber diet (p<0.01). It is concluded that addition of canola seed to high starch diet had favorable effects on growth performance and rumen pH but because of its negative effect on fiber digestibility and its different response depend on carbohydrate source, more research is needed for conclusion

The Protective Effect of Aloe Vera on Histological Structure of Endocrine Portion of Pancreas Gland in the Diabetic Rat

Introduction: Since aloe vera plant has many medical benefits, the present study aimed to investigate the protective effects of Aloe vera gel on the pancreatic islets and beta cells. Methods: This experimental study consisted of 50 mature male rats aged 2-3 months and weighed 200-250 g, who were randomly divided into five groups (n=10). Group I (control) did not receive any treatments, and group II were diabetized via Streptozotocin (IP) in 65 mg/kg, whose blood sample was taken after one week. Rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III diabetic rats received the Aloe vera gel daily with dosage of 400 mg/kg, and group IV diabetic rats received insulin in 10 units/rat. Group V involved healthy rats which received only Aloe vera gel. After the last Aloe vera gel administration, blood glucose and body weight of all groups were measured on 15th and 30th days. Animals were euthanized with ether. Then tissues samples were collected from pancreas gland and fixed in 10% neutral buffered formalin solution. The 5-6 &micro; sections were made by paraffin embedding method and stained using haematoxylin-eosin (H&E) and Aldehyde fuchsin stains. Ultimately, the histomorphometrical parameters were evaluated. Results: The mean number and size of pancreatic islets and beta cells of Langerhans islets decreased significantly in the diabetic group compared to the control group. The number of beta cells and diameter of langerhans islets increased significantly in the rats treated by Aloe vera gel in comparison to diabetic group at the end of 15th and 30th days. Conclusion: Applying Aloe vera gel seems to improve the renewal and restoration of langerhans islets and beta cells of pancreas gland in the diabetic rat

Study of Okra Powder (Abelmoscus Esculentus) Effects on Histology of Liver Tissue and Sero-Biochemical Parameters in Diabetic Rats (HFD/STZ)

Introduction: Type 2 diabete is a kind of metabolic disease that it is associated with hyperglycemia, hyperlipidemia and disturbed liver function.&nbsp; The aim of the present study was to evaluate the protective effects of Okra Powder on liver damage in high fat diet fed / streptozotocin (HFD-STZ)-induced type 2 diabetic rats. Methods: In this experimental study, 25 Wistar Albino female rats with an average weight of (200&ndash;220 g) were randomly divided&nbsp; into 5 groups: Group I: (control group) rats were fed the standard diet, Group II: healthy rats that received Okra Powder (200 mg/kg) for 4 weeks; Group III (HFD/STZ group): Rats were fed with high-fat diet (HFD) (60% fat) for 4 weeks&nbsp; and then injected low dose of STZ (35 mg/kg), Group IV:&nbsp; Diabetic rats that received Okra Powder (200 mg/kg) for 4 weeks. GroupV: Diabetic rats that received metformin (200 mg/kg) for 4 weeks. At the end of experiment, biochemical parameters were measured. Liver samples were removed and 5-6 &micro; sections were made and stained by H&E and Sudan black staining. Results: The results showed that all the biochemical parameters, except HDL-C and serum insulin were increased in diabetic rats, while they were decreased in Okra supplementation group compared&nbsp; to diabetic rats (p<0.05). The liver structure alterations were improved in treated diabetic rats with Okra Powder and metformin.&nbsp; Conclusion: Our findings confirmed the potential anti-hyperglycemic and hypolipidemic effects of Okra Powder. Thus, it seems it has an important role in the management of type 2 diabete