29 research outputs found

    A watershed and active contours based method for dendritic spine segmentation in 2-photon microscopy images (2-Foton mikroskopi görüntülerindeki dendritik dikenlerin bölütlenmesi için watershed ve etkin çevritlere dayalı bir yöntem)

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    Analysing morphological and volumetric properties of dendritic spines from 2-photon microscopy images has been of interest to neuroscientists in recent years. Developing robust and reliable tools for automatic analysis depends on the segmentation quality. In this paper, we propose a new segmentation algorithm for dendritic spine segmentation based on watershed and active contour methods. First, our proposed method coarsely segments the dendritic spine area using the watershed algorithm. Then, these results are further refined using a region-based active contour approach. We compare our results and the results of existing methods in the literature to manual delineations of a domain expert. Experimental results demonstrate that our proposed method produces more accurate results than the existing algorithms proposed for dendritic spine segmentation

    Combining nonparametric spatial context priors with nonparametric shape priors for dendritic spine segmentation in 2-photon microscopy images

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    Data driven segmentation is an important initial step of shape prior-based segmentation methods since it is assumed that the data term brings a curve to a plausible level so that shape and data terms can then work together to produce better segmentations. When purely data driven segmentation produces poor results, the final segmentation is generally affected adversely. One challenge faced by many existing data terms is due to the fact that they consider only pixel intensities to decide whether to assign a pixel to the foreground or to the background region. When the distributions of the foreground and background pixel intensities have significant overlap, such data terms become ineffective, as they produce uncertain results for many pixels in a test image. In such cases, using prior information about the spatial context of the object to be segmented together with the data term can bring a curve to a plausible stage, which would then serve as a good initial point to launch shape-based segmentation. In this paper, we propose a new segmentation approach that combines nonparametric context priors with a learned-intensity-based data term and nonparametric shape priors. We perform experiments for dendritic spine segmentation in both 2D and 3D 2-photon microscopy images. The experimental results demonstrate that using spatial context priors leads to significant improvements.Comment: IEEE International Symposium on Biomedical Imagin

    Automatic dendritic spine detection using multiscale dot enhancement filters and sift features

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    Statistical characterization of morphological changes of dendritic spines is becoming of crucial interest in the field of neurobiology. Automatic detection and segmentation of dendritic spines promises significant reductions on the time spent by the scientists and reduces the subjectivity concerns. In this paper, we present two approaches for automated detection of dendritic spines in 2-photon laser scanning microscopy (2pLSM) images. The first method combines the idea of dot enhancement filters with information from the dendritic skeleton. The second method learns an SVM classifier by utilizing some pre-labeled SIFT feature descriptors and uses the classifier to detect dendritic spines in new images. For the segmentation of detected spines, we employ a watershed-variational segmentation algorithm. We evaluate the proposed approaches by comparing with manual segmentations of domain experts and the results of a noncommercial software, NeuronIQ. Our methods produce promising detection rate with high segmentation accuracy thus can serve as a useful tool for spine analysis

    Disjunctive normal shape Boltzmann machine

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    Shape Boltzmann machine (a type of Deep Boltzmann machine) is a powerful tool for shape modelling; however, has some drawbacks in representation of local shape parts. Disjunctive Normal Shape Model (DNSM) is a strong shape model that can effectively represent local parts of objects. In this paper, we propose a new shape model based on Shape Boltzmann Machine and Disjunctive Normal Shape Model which we call Disjunctive Normal Shape Boltzmann Machine (DNSBM). DNSBM learns binary distributions of shapes by taking both local and global shape constraints into account using a type of Deep Boltzmann Machine. The samples generated using DNSBM look realistic. Moreover, DNSBM is capable of generating novel samples that differ from training examples by exploiting the local shape representation capability of DNSM. We demonstrate the performance of DNSBM for shape completion on two different data sets in which exploitation of local shape parts is important for capturing the statistical variability of the underlying shape distributions. Experimental results show that DNSBM is a strong model for representing shapes that are composed of local parts

    A joint classification and segmentation approach for dendritic spine segmentation in 2-photon microscopy images

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    Shape priors have been successfully used in challenging biomedical imaging problems. However when the shape distribution involves multiple shape classes, leading to a multimodal shape density, effective use of shape priors in segmentation becomes more challenging. In such scenarios, knowing the class of the shape can aid the segmentation process, which is of course unknown a priori. In this paper, we propose a joint classification and segmentation approach for dendritic spine segmentation which infers the class of the spine during segmentation and adapts the remaining segmentation process accordingly. We evaluate our proposed approach on 2-photon microscopy images containing dendritic spines and compare its performance quantitatively to an existing approach based on nonparametric shape priors. Both visual and quantitative results demonstrate the effectiveness of our approach in dendritic spine segmentation

    Biomedical image time series registration with particle filtering (Parçacık süzgeci ile biyomedikal görüntü zaman serisi çakıştırma)

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    We propose a family of methods for biomedical image time series registration based on Particle filtering. The first method applies an intensity-based information-theoretic approach to calculate importance weights. An effective second group of methods use landmark-based approaches for the same purpose by automatically detecting intensity maxima or SIFT interest points from image time series. A brute-force search for the best alignment usually produces good results with proper cost functions, but becomes computationally expensive if the whole search space is explored. Hill climbing optimizations seek local optima. Particle filtering avoids local solutions by introducing randomness and sequentially updating the posterior distribution representing probable solutions. Thus, it can be more robust for the registration of image time series. We show promising preliminary results on dendrite image time series
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