Alleviation of Aluminum-Induced Oxidative Stress, Trace Element, and Mineral Levels in Rat Tissues Protective Role of Pomegranate Juice (Punica Granatum L.)

The present investigation examined the impact of pomegranate (Punica granatum L.) juice on trace elements, minerals, and oxidative stress in relation to the potential harm inflicted by aluminum chloride (AlCl3) in rats. Rats were split into four groups at random for this purpose: control (C), pomegranate juice (PJ), aluminum chloride (A), and PJ + A. For 30 days, PJ was orally administered by gavage at a rate of 4 mL/kg every other day, whereas AlCl3 was administered intraperitoneally at 8.3 mg/kg. Spectrophotometric analysis was used to measure the levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) enzyme activity in various tissues. In addition, high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS) was used to determine the amounts of the elements Al, Cu, Fe, Mn, Zn, Ca, and Mg in the tissues. It was discovered that when PJ therapy was applied to all tissues, the antioxidant enzymes SOD and CAT activity increased, the GSH level rose, and the MDA level, a sign of lipid peroxidation, decreased. Al and Ca levels increased in the A group relative to the C group in all tissues, whereas they decreased in the A + PJ group relative to the A group. Group A exhibited a proportionate increase in Fe levels in the liver and renal tissues compared with group C. Furthermore, the A group’s brain tissue had a higher Fe level than the C group’s. The A + PJ group’s brain tissue had a lower Fe level than the A group’s. Our findings demonstrate that PJ therapy greatly decreased Al buildup and oxidative stress in tissues while controlling variations in trace element levels. In addition, it is concluded that PJ might have value as a strong chelating agent to prevent Al poisoning. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature

Evaluation of effects of bupivacaine and isoflurane on pancreas damage after renal ischemia-reperfusion injury: An experimental study

Recent studies have shown that renal ischemia-reperfusion injury can have detrimental effects on distant organs such as the brain, liver and lungs. In this study, we aimed to investigate the effects of renal ischemia-reperfusion injury on pancreatic functions. Materials and Methods. Twenty four male adult Wistar rats were divided into four groups. Sham and control group animals were not given any medications. Animals in groups 3 and 4 were treated with epidural bupivacaine and isoflurane inhalation. Animals in all groups except for the sham group were subjected to bilateral renal ischemia for 45 minutes and subsequent reperfusion. Blood samples were collected before ischemia, immediately after reperfusion and 2h after reperfusion. Serum blood urea nitrogen, creatinine, amylase and lipase levels were measured, and pancreatic sections were histopathologically examined for the presence and severity of congestion, degenerative cellular changes, cytoplasmic vacuolization and leukocytic infiltration. Levels of malondialdehyde, endogenous antioxidant enzyme catalase and reduced glutathione were measured in pancreatic tissue sections by using colorimetric kits. Results. Serum blood urea nitrogen and creatinine levels increased in rats subjected to renal ischemia-reperfusion. There was no difference between the groups in terms of pancreatic tissue malondialdehyde, catalase and glutathione levels. Conclusion. In conclusion, bilateral renal ischemia for 45 minutes led to significant impairment in pancreatic function and changes in pancreas histology. These findings might be due to antioxidant deficiency and increased lipid peroxidation in pancreatic tissue. © Erbesler Z.A., Ulcay T., 2021. All rights reserved