Aneuploidy Rate, DNA Fragmentation, Tubulin and Centrin Content in Different Groups of Male Infertility

In this study, we aimed to compare DNA fragmentation ratios, centrosomal protein concentrations (centrin and tubulin) and numerical chromosomal abnormalities between four groups including asthenozoospermia, teratozoospermia, oligoasthenozoospermia and normozoospermia patients, and to emphasize the necessity of investigating genetic and protein content of the spermatozoa also in patients with a semen analysis within normal reference range. Evaluation of semen analysis of the patients was performed based on WHO 2010 criteria. As a result of the statistical analysis, it was observed that the sperm DNA fragmentation rate in the normozoospermia group (6.50 ± 3.46%) was significantly lower than those in the teratozoospermia (19.69 ± 9.86%) and oligoasthenoteratozoospermia group (32.47± 14.13%) (p<0.001). For centrin and tubulin proteins, highest concentrations were observed in normozospermia group (p<0.05). When incidence of numerical abnormalities were compared between four groups, it was found that there were statistically significant differences between groups in terms of nullisomies X, Y and 13, and  21 (p<0.05). In conclusion, we suggest that abnormalities in semen parameters seem to be associated with chromosomal abnormalities; and sperm aneuploidy rate is also at a considerable level even in normozoospermic patients. Keywords: DNA fragmentation, teratozoospermia, centrin, tubulin DOI: 10.7176/JMPB/68-04 Publication date:September 30th 2020

Maternal and Neonatal Outcomes Related to Iron Deficiency Anemia and Serum Ferritin Status: A Multicenter Prospective Study From Eastern Marmara, Turkey

Aim:The aim was to evaluate the incidence of iron deficiency anemia in pregnancy in the East Marmara region of Turkey in order to determine its prevalence along with the effects and associations of iron supplementation on maternal and neonatal outcomes.Materials and Methods:This study was conducted in six centers and included a total of 1102 pregnant women. Blood samples were collected for hematological status and serum ferritin levels during pregnancy, and the adverse maternal and perinatal outcomes were determined. Iron deficiency anemia was diagnosed according to the World Health Organization criteria as hemoglobin level of < 11 g/dl and ferritin level of <15 μg/dL.Results:The rate of anemia was 19.8%, with 44% of them receiving iron supplementation. The maternal age was lower in the anemic group (26.5 vs. 27.7, p = 0.01). Selective iron use was more frequent in the anemic group, while routine iron use was more frequent in the non-anemic group (47.1% vs. 29.3%; p = 0.01).Conclusion:Iron deficiency anemia is a frequent problem in pregnancy. However, many anemic pregnant women do not receive iron therapy. Iron supplementation may have positive effects on maternal and perinatal outcomes. In order to combat iron deficiency anemia in pregnancy, wide spread use of iron supplements should be established