Singlet oxygen formation during accelerated and hyperaccelerated corneal cross-linking: in vitro study

BACKGROUND: To evaluate the singlet oxygen (1 O2) production of oxygen assisted %0.1 riboflavin and ultraviolet-A (UVA) crosslinking therapy (with and without oxygen assistance), in combination with standard, accelerated and hyper-accelerated procedures via an important quantitive marker of 1 O2 which is the photo-oxidation of 1,3 diphenylisobenzofuran (DPBF). METHODS: %0.1 riboflavin-containing wells were irradiated with UVA light (365-nm wavelength) with or without 2-4-6-8 L/min oxygen flow assistance. Measurements of decrease in absorbance of DPBF were made in 30 mW (hyper-accelerated), 9 mW (accelerated), and 3 mW UV-A (standard) applications, and with additional 2-4-6-8 L/min oxygen flow in 30 mW and 2 L/min oxygen flow in 9 mW. A total of 8 different UV-A irradiance with and without oxygen supplementation groups were formed. RESULTS: 2 L/min oxygen assisted accelerated UV-A irradiance group has shown a greater decrease in DPBF absorbance compared to Dresden protocol. (p = 0.014) Also, Dresden protocol has shown a greater decrease in DPBF compared to all groups except accelerated crosslinking with 2 L/min oxygen. (p < 0.001) Oxygen assisted hyper-accelerated crosslinking groups were showed greater reduction in DPBF absorbance compared to standard crosslinking without oxygen groups. (p < 0.001). CONCLUSION: Oxygen supplementation may increase the singlet oxygen generation to the similar levels of Dresden Protocol’s in accelerated group. Also, more singlet oxygen generation with oxygen supplementation compared to standard UV-A application might be considered to be promising in terms of shortening the crosslinking therap

The effect of carbon monoxide releasing molecule-2 (Corm-2) on healing of ischemic colon anastomosis in rats

Background/aim: Ischemia on the colon wall negatively affects healing of anastomosis. We were aimed to evaluate the effects of carbon monoxide releasing molecule-2 (CORM-2) on the healing of anastomosis in a rat model of the ischemic colon. Materials and methods: In this prospective study a total of 60 rats were randomly divided into three groups as colon transection and end-to-end anastomosis (Group I), colon transection, and end-to-end anastomosis following the induction of ischemia (Group II), and colon transection and end-to-end anastomosis following the induction of ischemia and treated with daily intraperitoneal administration of CORM-2 (Group III). Each group was also divided into two equal subgroups as postoperative 3rd and 7th day. Postoperative healing of anastomoses was evaluated by anastomosis burst pressure (ABP), tissue biomarkers including hydroxyproline (HP), malondialdehyde (MDA), glutathione (GSH), and histopathological findings. Results: In the ischemic group treated with CORM-2, lower MDA and higher HP levels were observed in comparison to the untreated ischemic group on the 3rd day. GSH and HP levels were higher and MDA levels was lower in the ischemic rats treated with CORM-2 than in the ischemic untreated rats on the 7th day. In the ischemic group treated with CORM-2, the mucosal epithelial score decreased and the neoangiogenesis score increased compared to the untreated rats on the 7th day. Conclusion: In ischemic colon anastomosis, reduces cell destruction by suppressing the oxidative reaction, and strengthening the antioxidative mechanisms of the cells. It also increases collagen formation, epithelial development, and neoangiogenesis

Serum levels of interleukin-6 are correlated to the angiographic extent and severity of coronary artery disease

29th Turkish Cardiology Congress of the Turkish-Society-of-Cardiology (TSC) with International Participation -- OCT 26-29, 2013 -- Antalya, TURKEYTurkish Society of Cardiolog

Distribution of thalassemia trait in Balikesir Province according to trait type and age group

Objectives: The aim of this study was to determine the prevalence of the thalassemia disease group in Balikesir Prov ince, Turkey. Methods: Blood samples provided by 11,558 individuals (5675 males and 5883 females) aged 3-90 years between May 15, 2018 and September 30, 2019 for laboratory analysis at the Balikesir Provincial Public Health Laboratory were included in the study. Hemoglobin chain analyses were carried out using the high-performance liquid chromatography (HPLC) method. The data obtained were analyzed retrospectively. Results: The level of hemoglobin (Hb) A2 was >3.5% in 591 (5.11%) of the total study group and these individuals were identified as ?-thalassemia carriers. The prevalence of the ?-thalassemia trait was 5.76% in females and 4.44% in males. A total of 792 cases (446 female and 346 male) had a result outside the normal range: 74.6% were identified as thalassemia carriers, 9.4% had isolated low Hb A2, 12.3% had isolated Hb F elevation, and 3.8% had total abnormal hemoglobin values. Conclusion: The prevalence of ?-thalassemia trait (5.11%) in the study group was extrapolated for the general pop ulation of Balikesir (2019 population: 1,228.620 ) and it was estimated that there were 62,782 potential carriers in the province

A comparative study on the performances of 77 elektronika urised 2-LabUmat2 and Dirui FUS200 - H800 urine analyzers

Objectives: This study aims to compare the analytical performance of the 77Elektronika brand LabUmat2-Urised2 model integrated urine analyzer and Dirui brand FUS200-H800 model integrated urine analyzer.Methods: Urine samples of 139 patients randomly selected from male and female patients of all age groups who were admitted to Ufuk University Faculty of Medicine, Dr. Ridvan Ege Hospital between 24.02.2020-28.02.2020 were analyzed on both devices.Results: The results of the samples whose WBC (white blood cell), RBC (red blood cell) and Squamous epithelial were measured using two different methods in microscopic analyzers were perfectly compatible in the same range and with three parameters (Gamma values 0.916; 0.770; 0.961, respectively). For the samples where crystal and cylinder tests were carried out in microscopic analyzers, Cappa values were 0.486 and 0.495, respectively. As the result of Passing-Bablok Regression analysis used for the method comparison of microscopic analyzers, Cusum test results were p<0.01, p=0.01 and p=0.65, respectively for the linearity in the measurement of WBC, RBC and Squamous epithelial. According to the Bland-Altman Compatibility Chart prepared to determine the difference between methods, the difference between the results of the measurements of WBC, RBC and Squamous epithelial in both devices were -29.3±1.96 SD (95% CI, lower limit: -174.6; upper limit: 116); 43.3±1.96 SD (95% CI, lower limit: -119.7; upper limit: 206.2); -4.0±1.96 SD (95% CI, lower limit: -87.8%; upper limit: 79.9), respectively. When the results of the chemical analyzers of each device were compared, the findings showed that there was a high correlation between leukocyte and protein levels. When the compliance of chemical and microscopic units of each device was examined, a high correlation was found for WBC and a medium level correlation for RBC.Conclusion: Although both devices revealed similar results, confirmation with the manual microscope might be required, especially in pathological samples. Therefore, microscopic analyzers are still needed to be developed concerning method and software although automatic urine analyzers provide standardization and reduce the workload of laboratories