Historical, Archaeological, and Geophysical Investigations at Two Proposed Safety Rest Areas, Interstate Highway (IH) 10, Chambers County, Texas

Personnel from Coastal Environments, Inc. (CEI), Moore Archeological Consulting, Inc. (MAC), and the University of Mississippi conducted archaeological and geophysical investigations at the locations of two proposed safety rest areas on opposite sides of Interstate Highway (IH) 10 in Chambers County, Texas. The research was carried out from late August 2006 until late February 2007, under contract to the Environmental Affairs Division of the Texas Department of Transportation (TxDOT). MAC archaeologists had previously examined the two rest area tracts in 2001. Their research indicated that the north tract contained a latenineteenth- through early-twentieth-century cemetery, identified as the Broussard Cemetery site (41CH370). Buried within the cemetery are the remains of several members of the locally prominent White family and relatives. The south tract included the remains of a below-ground cistern that likely marked the location of the main house associated with the homestead and ranch of James Taylor White II. It was estimated that this house location, labeled the White Family Cistern site (41CH371), was occupied from ca. 1854 until sometime in the early 1900s. The field investigations examined three specific areas within the two tracts: (1) A small 20-by-45-m area situated about 10 m north of the Broussard Cemetery site where a truck-entrance road is to be built. It was considered possible that unmarked graves located outside the cemetery proper might be present in that area. (2) A 40-by-40-m area within the south tract where MAC personnel had located a piece of whiteware during their earlier investigations. It was thought that a possible outbuilding related to the White homestead might be present in that area. (3) A 110-by-115-m area in the south tract where the main house and most of the White family occupation occurred. The area in the north tract was examined by ground-penetrating radar, resistivity surveys and mechanical stripping of anomalies recognized by the geophysical research. The small square area in the south tract was examined by systematic shovel tests. The large area in the south tract was investigated by systematic shovel tests, a metal detector survey, a geophysical search that included magnetometer and electromagnetic susceptibility surveys, a limited ground-truth assessment of selected anomalies that had been identified by the geophysical surveys, mechanical stripping of other anomalies recognized by the geophysical research, plus the controlled excavation of a few small units in locations where the stripping uncovered potential cultural features. Overall, the various investigations identified the location, orientation, and dimensions of the White family house and its associated kitchen, a rich sheet midden situated to the rear of the house, and several possible outbuildings located to the sides of the structure. Numerous artifacts indicative of the period of suspected occupation were collected, including hundreds of pieces of metal, ceramic fragments, and glass. A few animal bones and plant remains also were obtained. Although the present study did not call for a detailed analysis of these items, such should prove useful in the future. Given these results, it is clear that site 41CH371 is eligible for inclusion in the National Register of Historic Places. Additional archaeological investigations at selected portions of the site are recommended, along with further archival and historical research. Accordingly, construction of the rest area in the south tract should be delayed until the recommended investigations are completed. The small area examined in the north tract near site 41CH370 failed to yield any evidence of burials. Since the cemetery itself will be avoided during construction, no further archaeological work is considered necessary in the north tract. Thus, construction of the rest area within the north tract may proceed as planned

Cross-Reactivity of Herpesvirus-Specific CD8 T Cell Lines Toward Allogeneic Class I MHC Molecules

Although association between persistent viral infection and allograft rejection is well characterized, few examples of T-cell cross-reactivity between self-MHC/viral and allogeneic HLA molecules have been documented so far. We appraised in this study the alloreactivity of CD8 T cell lines specific for immunodominant epitopes from human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). CD8 T cell lines were generated after sorting with immunomagnetic beads coated with either pp65495–503/A*0201, BMLF1259–267/A*0201, or BZLF154–64/B*3501 multimeric complexes. Alloreactivity of the CD8 T cell lines against allogeneic class I MHC alleles was assessed by screening of (i) TNF-α production against COS-7 cells transfected with as many as 39 individual HLA class I-encoding cDNA, and (ii) cytotoxicity activity toward a large panel of HLA-typed EBV-transformed B lymphoblastoid cell lines. We identified several cross-reactive pp65/A*0201-specific T cell lines toward allogeneic HLA-A*3001, A*3101, or A*3201. Moreover, we described here cross-recognition of HLA-Cw*0602 by BZLF1/B*3501-specific T cells. It is noteworthy that these alloreactive CD8 T cell lines showed efficient recognition of endothelial cells expressing the relevant HLA class I allele, with high level TNF-α production and cytotoxicity activity. Taken together, our data support the notion that herpes virus-specific T cells recognizing allo-HLA alleles may promote solid organ rejection

ISPMD consensus on the management of premenstrual disorders

The second consensus meeting of the International Society for Premenstrual Disorders (ISPMD) took place in London during March 2011. The primary goal was to evaluate the published evidence and consider the expert opinions of the ISPMD members to reach a consensus on advice for the management of premenstrual disorders. Gynaecologists, psychiatrists, psychologists and pharmacologists each formally presented the evidence within their area of expertise; this was followed by an in-depth discussion leading to consensus recommendations. This article provides a comprehensive review of the outcomes from the meeting. The group discussed and agreed that careful diagnosis based on the recommendations and classification derived from the first ISPMD consensus conference is essential and should underlie the appropriate management strategy. Options for the management of premenstrual disorders fall under two broad categories, (a) those influencing central nervous activity, particularly the modulation of the neurotransmitter serotonin and (b) those that suppress ovulation. Psychotropic medication, such as selective serotonin reuptake inhibitors, probably acts by dampening the influence of sex steroids on the brain. Oral contraceptives, gonadotropin-releasing hormone agonists, danazol and estradiol all most likely function by ovulation suppression. The role of oophorectomy was also considered in this respect. Alternative therapies are also addressed, with, e.g. cognitive behavioural therapy, calcium supplements and Vitex agnus castus warranting further exploration

Development of Conceptual Models to Guide Public Health Research, Practice, and Policy: Synthesizing Traditional and Contemporary Paradigms

This applied paper is intended to serve as a "how to" guide for public health researchers, practitioners, and policy makers who are interested in building conceptual models to convey their ideas to diverse audiences. Conceptual models can provide a visual representation of specific research questions. They also can show key components of programs, practices, and policies designed to promote health. Conceptual models may provide improved guidance for prevention and intervention efforts if they are based on frameworks that integrate social ecological and biological influences on health and incorporate health equity and social justice principles. To enhance understanding and utilization of this guide, we provide examples of conceptual models developed by the Prevention of Lower Urinary Tract Symptoms (PLUS) Research Consortium. PLUS is a transdisciplinary U.S. scientific network established by the National Institutes of Health in 2015 to promote bladder health and prevent lower urinary tract symptoms, an emerging public health and prevention priority. The PLUS Research Consortium is developing conceptual models to guide its prevention research agenda. Research findings may in turn influence future public health practices and policies. This guide can assist others in framing diverse public health and prevention science issues in innovative, potentially transformative ways

Non-invasive bladder function measures in healthy, asymptomatic female children and adolescents: A systematic review and meta-analysis

BackgroundLower urinary tract symptoms (LUTS) are common in children and adolescents. Non-invasive tests evaluating bladder function are generally preferred over invasive tests, yet few studies have explored the range of normative values for these tests in healthy, asymptomatic children.ObjectiveTo define normative reference ranges for non-invasive tests of bladder function in healthy, asymptomatic girls and adolescents.Study designA comprehensive search strategy was performed in seven electronic databases through October 2019. English-language studies reporting data on voiding frequency, voided and postvoid residual volumes (PVR) and uroflowmetry results in healthy, asymptomatic girls (mean age ≥ 5 years) were included. Two independent reviewers performed study review, data extraction, and quality assessment. Overall mean estimates and 95% confidence intervals for each bladder function parameter were calculated using random effects models, and 95% normative reference values were estimated.ResultsTen studies met eligibility criteria for the meta-analysis (n = 2143 girls, age range: 3-18). Mean estimates of maximum voided volume and PVR were 233.4 ml (95% CI 204.3-262.6; n = 1 study) and 8.6 ml (95% CI 4.8-12.4; n = 2 studies) respectively. Pooled mean estimates for uroflowmetry parameters were: 21.5 ml/s (95% CI 20.5-2.5) for maximum flow rate (n = 6 studies), 12.5 ml/s (95% CI 11.2-13.8) for mean flow rate (n = 6 studies), 6.8 s (95% CI 4.4-9.3) for time to maximum flow (n = 3 studies), 15.7 s (95% CI 13.0-18.5) for flow time (n = 3 studies), and 198.7 ml (95% CI 154.2-234.2) for voided volume (n = 9 studies). No studies reported estimates of voiding frequency. Between-study heterogeneity was high (89.0-99.6%).ConclusionsAlthough we were able to calculate pooled mean estimates for several parameters, the small number of included studies and the wide age ranges of participants preclude generalization of reference values to all healthy girls. Further research is needed to determine normative reference values within specific age groups