Mechanical properties, water sorption characteristics, and compound release of grape seed extract-incorporated resins

published_or_final_versio

Anti-collagenolytic activity of proanthocyanidin

Theme: Dental Treatment and Restorative Dentistry - Materials (Oral Presentation)This journal suppl. entitled: Special Issue: Abstracts of the 2012 FDI Annual World Dental CongressBACKGROUND: Resin-dentine bond has been shown to be less durable due to collagen degradation within hybrid layer by matrix metalloprotenases (MMPs) and cysteine cathepsins. OBJECTIVE: This study evaluated the anti-protease effect of proanthocyanidin (PA) on soluble and matrix-bound MMPs and cysteine cathep...postprin

Application of immortalized mouse dental papilla cells for tooth bioengineering

published_or_final_versio

Effect of solvent on the inhibition of MMP by chlorhexidine

Poster Session - Dental Materials 1: Adhesion - Bond Strength Testing and Mechanisms: abstract no. 1867OBJECTIVES: Chlorhexidine (CHX) is a commonly used matrix metalloproteinases (MMP) inhibitor to improve resin-dentin bond durability. Several solvents have been used to solvate CHX for clinical applications. This study evaluated the inhibitory effect of CHX in different solvents on soluble and matrix-boun...link_to_OA_fulltex

Effect of proanthocyanidin incorporation on mechanical properties of adhesive resin

Abstract in https://iadr.confex.com/iadr/sea12/webprogrampreliminary/Paper169571.htmlScientific Groups: Dental Materials - paper 16957

Effect of additional acid etching on bond strength of self-etch adhesive to dentine affected with Amelogenesis Imperfecta

The HKU Faculty of Dentistry and Sun Yat-sen University Guanghua School of Stomatology Conjoint Scientific Meeting and 1st Guangdong - Hong Kong Postgraduate Forum in Dental Science, Guangdong, China, 14-16 December 2010

Synergistic effect of proanthocyanidin and CPP-ACFP on root caries remineralisation

Poster Session: Dentin Morphology, Occlusion, RemineralizationObjective: Root caries remains a major oral health problem among the elderly. Proanthocyanidin (PA) has shown positive effects on remineralization of artificial root caries. It is advantageous to find methods to enhance the remineralization effect of PA. This study evaluated the synergistic effect of PA and casein phosphopeptide amorphous calcium fluoride phosphate (CPP-ACFP) on remineralization of artificial root caries. Method: Demineralized root fragments (n=90) were randomly divided into six groups based on treatments: (i) 6.5% PA, (ii) CPP-ACP, (iii) CPP-ACFP, (iv) CPP-ACFP + 6.5% PA, (v) 1000ppm fluoride and (vi) deionized water (control). Each specimen was immersed in the demineralizing solution for 14 h, testing solutions/pastes for 2 h and remineralizing solution for 8 h. The pH cycling was performed at 37oC for 8 days. Specimens were evaluated using transverse microradiography (lesion depth and mineral loss), a microhardness tester (Knoop hardness) and confocal laser scanning microscopy (CLSM). The lesion depth and mineral loss were analyzed using one-way ANOVA; while microhardness were analyzed with two-way ANOVA and Tukey’s test. Result: One-way ANOVA indicated that testing solutions had a significant effect on lesion depth (P<0.05) and mineral loss (P<0.05). Lesion depth was significantly lower in CPP-ACFP+PA and 1000ppm fluoride groups (P<0.05). Mineral loss was the lowest in CPP-ACFP+PA group (P<0.05). Two-way ANOVA indicated that the factors “testing solutions/pastes” and “lesion depth” had a significant effect on Knoop hardness (P<0.05), with interaction between the two factors (P<0.05). Artificial caries lesions treated with CPP-ACFP+PA showed significantly higher microhardness values at 130μm and 150 μm from the surface (P<0.05). Remineralizing bands with higher mineral density were observed in CPP-ACFP+PA group. Conclusion: The combined use of proanthocyanidin and casein phosphopeptide amorphous calcium fluoride phosphate has a synergistic effect on root caries remineralization by enhancing mineral gain and increasing hardness of the artificial root caries

Effects of Flavonoids on Artificial Root Caries Remineralization

Session: Cariology ResearchObjectives: Root caries is a major oral health problem among the elderly. It is advantageous to find ways to enhance remineralization of root caries.This study compared the effects of three flavonoids, proanthocyanidin, naringin and quercetin on artificial root caries remineralization and collagen mineralization. Methods: Caries lesions created artificially from seventy-five root fragments were subjected to a pH cycling model. Demineralized root fragments were randomly divided into 5 groups for treatment with the remineralizing agents for 10 minutes: (i) 6.5% proanthocyanidin, (ii) 6.5% naringin, (iii) 6.5% quercetin, (iv) 1000ppm fluoride and (v) deionized water (control). The remineralization effects were evaluated using transverse microradiography (lesion depth and mineral loss), confocal laser scanning microscopy and microhardness. The lesion depth and mineral loss were analyzed with one-way ANOVA; while microhardness at different lesion depths were analyzed with two-way ANOVA and Tukey’s test. For observation of collagen mineralization, TEM grids coated with Type I collagen fibrils were divided into 4 groups for treatment with remineralizing agents for 1 hour: (i) 6.5% proanthocyanidin, (ii) 6.5% naringin, (iii) 6.5% quercetin and (iv) 1000ppm fluoride. Collagen fibrils were then incubated in the remineralization medium for 72 hours. Finally the collagen-coated grids were washed, dried and observed under Transmission Electron Microscopy. Results: The lesion depths and mineral loss of flavonoid-treated groups were both significantly lower than the control group, but higher than the fluoride-treated group (P0.05). Artificial caries lesions treated with fluoride and flavonoids showed significantly greater hardness than control group (P<0.05). Different degrees of mineral deposition were observed in flavonoid-treated collagen. Conclusions: All three flavonoids showed positive effects on artificial root caries remineralization and collagen mineralization. (This study was supported by Seed Funding Programme for Basic Research 201011159189