75 research outputs found
Measurements of interrupter resistance: reference values for children 3-13 yrs of age
The interrupter technique is a convenient and sensitive technique for
studying airway function in subjects who cannot actively participate in
(forced) ventilatory function tests. Reference values for preschool
children exist but are lacking for children >7 yrs. Reference values were
obtained for expiratory interrupter resistance (R(int,e)) in 208 healthy
Dutch Caucasian children 3-13 yrs of age. A curvilinear relationship
between R(int,e) and height was observed, similar to published airways
resistance data measured by plethysmography. No significant differences in
cross-sectional trend or level of R(int,e) were observed according to sex.
It was found that Z-scores could be used to express individual R(int,e)
values and to describe intra- and interindividual differences based on the
reference equation: 10logR(int,e)=0.645-0.00668x standing height (cm) kPa
x L(-1) x s(-1) and residual SD (0.093 kPa x L(-1) x s(-1)). Expiratory
interrupter resistance provides a tool for clinical and epidemiological
assessment of airway function in a large age range
Rectal Organoids Enable Personalized Treatment of Cystic Fibrosis
In vitro drug tests using patient-derived stem cell
cultures offer opportunities to individually select
efficacious treatments. Here, we provide a study
that demonstrates that in vitro drug responses in
rectal organoids from individual patients with cystic
fibrosis (CF) correlate with changes in two in vivo
therapeutic endpoints. We measured individual
in vitro efficaciousness using a functional assay
in rectum-derived organoids based on forskolininduced swelling and studied the correlation with
in vivo effects. The in vitro organoid responses correlated with both change in pulmonary response and
change in sweat chloride concentration. Receiver
operating characteristic curves indicated good-toexcellent accuracy of the organoid-based test for
defining clinical responses. This study indicates
that an in vitro assay using stem cell cultures can prospectively select efficacious treatments for patients
and suggests that biobanked stem cell resources
can be used to tailor individual treatments in a
cost-effective and patient-friendly manner
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