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Recension av Ivar Arpis och Anna-Karin Wyndhamns bok Genusdoktrinen d\ue4r Chalmers m fl l\ue4ros\ue4ten beskylls f\uf6r att kvotera in kvinnor bakom lyckta d\uf6rrar

A LCMS Metabolomic Workflow to Investigate Metabolic Patterns in Human Intestinal Cells Exposed to Hydrolyzed Crab Waste Materials

We have developed a LCMS metabolomic workflow to investigate metabolic patterns from human intestinal cells treated with simulated gastrointestinal-digested hydrolyzed crab waste materials. This workflow facilitates smart and reproducible comparisons of cell cultures exposed to different treatments. In this case the variable was the hydrolysis methods, also accounting for the GI digestion giving an output of direct correlation between cellular metabolic patterns caused by the treatments. In addition, we used the output from this workflow to select treatments for further evaluation of the Caco-2 cell response in terms of tentative anti-inflammatory activity in the hopes to find value in the crab waste materials to be used for food products. As hypothesized, the treatment identified to change the cellular metabolomic pattern most readily, was also found to cause the greatest effect in the cells, although the response was pro-inflammatory rather than anti-inflammatory, it proves that changes in cellular metabolic patterns are useful predictors of bioactivity. We conclude that the developed workflow allows for cost effective, rapid sample preparation as well as accurate and repeatable LCMS analysis and introduces a data pipeline specifically for probe the novel metabolite patterns created as a means to assess the performing treatments

Toward celiac-safe foods - Investigation of the interaction between transglutaminase 2 and gluten

Celiac disease, a chronic autoimmune enteropathy, may develop in genetically predisposed individuals upon ingestion of gluten proteins found in wheat, barley, and rye. Overall prevalence of celiac disease is increasing and it currently affects around 1% of the population. The types and severity of symptoms of celiac disease show high variability, leading to many sufferers remaining undiagnosed. The only available treatment is to follow a strict gluten-free diet, but gluten-free alternatives are less available, more expensive, and often have lower nutritional and sensorial quality.This thesis work examined the interactions between the intestinal enzyme transglutaminase 2 and gluten peptides. Transglutaminase 2 plays a significant role in disease initiation and progression, and is the main autoimmune target in developed celiac disease. A method for measuring the interaction between transglutaminase 2 and gluten was developed and tested in studies on sourdough fermentation of wheat flour and bread. Transglutaminase 2-mediated transamidation of gluten was assayed and the extent of available binding motifs for transglutaminase 2 in α2-gliadin, considered the most immunogenic part of gluten, was assessed using an ELISA-based method. The results showed that lactic acid fermentation, which is not specifically tailored to degrade gluten, cannot sufficiently prevent transglutaminase 2 interaction with gluten or decrease the extent of available binding motifs for transglutaminase 2 on α2-gliadin. In studies investigating the possibility to block specific binding motifs for transglutaminase 2 on gluten peptides, using molecules suitable as food additives, binding to α2-gliadin was computationally simulated and promising candidates were identified. These candidates were analyzed in vitro for the ability to prevent transglutaminase 2-mediated transamidation and deamidation of gliadin. Ascorbyl palmitate was found to interact with α2-gliadin in computer simulations and effectively reduced gliadin interaction with transglutaminase 2 in vitro. The cytotoxicity profile of ascorbyl palmitate, in combination with gliadin, was evaluated in Caco-2 cell cultures by determining cell survival, direct cytotoxicity, inflammatory mediators, and cell layer integrity, and no negative effects were found. In ancillary studies of human ileostomy contents after ingestion of raw and extruded gluten-containing products, degradation products of α-gliadin were identified and the effect of extrusion on digestion was investigated. Preliminary results indicate that protein digestibility was decreased after intake of the extruded product, but the effect on α-gliadin digestion needs further evaluation. However, the majority of α-gliadin seems to be undigested after in vivo digestion in both products.The interaction between transglutaminase 2 and gluten is crucial for celiac disease and in this thesis work, several strategies for preventing this have been explored. Ascorbyl palmitate has been shown to effectively prevent this interaction in vitro and is thus a promising candidate for creating cereal-based foods potentially safe for celiacs

FORTE: an extensible framework for robustness and efficiency in data transfer pipelines

In the age of big data and growing product complexity, it is common to monitor many aspects of a product or system, in order to extract well-founded intelligence and draw conclusions, to continue driving innovation. Automating and scaling processes in data-pipelines becomes essential to keep pace with increasing rates of data generated by such practices, while meeting security, governance, scalability and resource-efficiency demands.We present FORTE, an extensible framework for robustness and transfer-efficiency in data pipelines. We identify sources of potential bottlenecks and explore the design space of approaches to deal with the challenges they pose. We study and evaluate synergetic effects of data compression and in-memory processing as well as task scheduling, in association with pipeline performance.A prototype implementation of FORTE is implemented and studied in a use-case at Volvo Trucks for high-volume production-level data sets, in the order of magnitude of hundreds of gigabytes to terabytes per burst. Various general-purpose lossless data compression algorithms are evaluated, in order to balance compression effectiveness and time in the pipeline.All in all, FORTE enables to deal with trade-offs and achieve benefits in latency and sustainable rate (up to 1.8 times better), effectiveness in resource utilisation, all while also enabling additional features such as integrity verification, logging, monitoring and traceability, as well as cataloguing of transferred data. We also note that the resource efficiency improvements achievable with FORTE, and its extensibility, can imply further benefits regarding scheduling, orchestration and energy-efficiency in such pipelines

Differential Effects of Iron Chelates vs. Iron Salts on Induction of Pro-Oncogenic Amphiregulin and Pro-Inflammatory COX-2 in Human Intestinal Adenocarcinoma Cell Lines

We previously showed that two iron compounds that are orally ingested by humans, namely ferric EDTA and ferric citrate, can induce an oncogenic growth factor (amphiregulin) in human intestinal epithelial adenocarcinoma cell lines. Here, we further screened these iron compounds, plus four other iron chelates and six iron salts (i.e., 12 oral iron compounds in total), for their effects on biomarkers of cancer and inflammation. Ferric pyrophosphate and ferric EDTA were the main inducers of amphiregulin and its receptor monomer, IGFr1. Moreover, at the maximum iron concentrations investigated (500 \ub5M), the highest levels of amphiregulin were induced by the six iron chelates, while four of these also increased IGfr1. In addition, we observed that ferric pyrophosphate promoted signaling via the JAK/STAT pathway by up-regulating the cytokine receptor subunit IFN-γr1 and IL-6. For pro-inflammatory cyclooxygenase-2 (COX-2), ferric pyrophosphate but not ferric EDTA elevated intracellular levels. This, however, did not drive the other biomarkers based on COX-2 inhibition studies and was probably downstream of IL-6. We conclude that of all oral iron compounds, iron chelates may particularly elevate intracellular amphiregulin. Ferric pyrophosphate additionally induced COX-2, probably because of the high IL-6 induction that was observed with this compound

The potential of vehicle and road infrastructure interventions in fatal bicyclist accidents on Swedish roads—What can in-depth studies tell us?

Objective: The objective of this article is to describe the characteristics of fatal crashes with bicyclists on Swedish roads in rural and urban areas and to investigate the potential of bicycle helmets and different vehicle and road infrastructure interventions to prevent them. The study has a comprehensive approach to provide road authorities and vehicle manufacturers with recommendations for future priorities. Methods: The Swedish Transport Administration’s (STA) in-depth database of fatal crashes was used for case-by-case analysis of fatal cycling accidents (2006–2016) on rural (n = 82) and urban (n = 102) roads. The database consists of information from the police, medical journals, autopsy reports, accident analyses performed by STA, and witness statements. The potential of helmet use and various vehicle and road infrastructure safety interventions was determined retrospectively for each case by analyzing the chain of events leading to the fatality. The potential of vehicle safety countermeasures was analyzed based on prognoses on their implementation rates in the Swedish vehicle fleet. Results: The most common accident scenario on rural roads was that the bicyclist was struck while cycling along the side of the road. On urban roads, the majority of accidents occurred in intersections. Most accidents involved a passenger car, but heavy trucks were also common, especially in urban areas. Most accidents occurred in daylight conditions (73%). Almost half (46%) of nonhelmeted bicyclists would have survived with a helmet. It was assessed that nearly 60% of the fatal accidents could be addressed by advanced vehicle safety technologies, especially autonomous emergency braking with the ability to detect bicyclists. With regard to interventions in the road infrastructure, separated paths for bicyclists and bicycle crossings with speed calming measures were found to have the greatest safety potential. Results indicated that 91% of fatally injured bicyclists could potentially be saved with known techniques. However, it will take a long time for such technologies to be widespread. Conclusions: The majority of fatally injured bicyclists studied could potentially be saved with known techniques. A speedy implementation of important vehicle safety systems is recommended. A fast introduction of effective interventions in the road infrastructure is also necessary, preferably with a plan for prioritization

A lidar-only SLAM algorithm for marine vessels and autonomous surface vehicles

Research into autonomous surface vehicles is noticeably limited in regards to the functionality of the vehicles themselves. Specifically, testing and evaluation typically occurs at speeds considerably lower than what is allowed in an operational setting. For a vessel to be able to take advantage of higher speeds, there must be a robust and tested method for determining localisation and navigation. With an emphasis of development for small vessels with higher impulse capabilities, working in confined and restricted environments, the decision was made to develop a method of navigation that relied solely upon lightweight sensors. For this, a single light ranging sensor was utilised to develop both simultaneous localisation and mapping for the vessel, using the normal distribution transform and iterative closest point methods. Evaluation of the algorithm accuracy as the vessel moved above speeds greater than two metres per second was conducted, and it was feasibly evaluated that there was no observable drift of mapping in horizontal planes, however, there was a accumulated drift in the vertical plane and a transient response in localisation deviation as the vessel changed impulse through the two metre per second window

SolVis: A pilot study to facilitate understanding of real-time solar energy production through energy visualizations in learning environments

The transition to a carbon neutral society requires actions on all levels of society. Hereby, renewable energy - especially considering the current European energy crisis – such as solar energy will play an increasing role. To promote a more extensive use of solar energy and thus contributing to a faster energy transition, it is valuable to engage all citizens, not least children. How can real-time visualization of solar energy production in learning environmentscontribute to increased understanding of energy production and consumption? Educational environments can be a powerful platform to cultivate an interest in more environmentally andsustainable behaviour at an early age. However, energy is intangible, abstract, and difficult to grasp for non-experts. Digital tools incorporating energy visualization may be able to facilitate an increased understanding of energy units and measurements. The City of Gothenburg, Sweden, has the ambition to place photovoltaics on all roofs of schools and kindergartens, a process that is already in progress. The solar energy production is measured in real-time and only used for building operation management. This paper presents a study that develops a concept for a digital and educational visualization tool for school children aged 9 to 12, with the goal to engage, facilitate a lasting learning outcome, and awaken curiosity in solar energy. A prototype of a real-time visualization tool was developed, which in a playful way visualises the solar electricity production of the school property and compares it to the energy consumption of everyday activities, displayed on a centrally placed screen in the school. It was installed in four pilot schools and evaluated with 233 pupils. Results indicate several potentials of the visualization: to communicate local solar energy production to pupils, triggering thoughts, ideas, and a will to continue to learn more about solar energy production

Drivers and Barriers for Inland Waterway Transportation

Inland waterway transportation (IWT) in Sweden could be a substitute for road transports with the prospects of improving the environmental performance. Sweden currently has no systematic strategies or policies for transports on inland waterways (IWW), and despite available capacity the waterways are barely utilized. In the Netherlands, for example, the IWW capacity is embedded in the transport system and utilized to a large extent. For a successful modal shift it is important to understand the drivers and barriers for the shift and develop strategies to leverage the drivers and mitigate the barriers. This study aims to identify drivers and barriers for IWT based on successful benchmark cases in the Netherlands. Furthermore, based on the learning from these benchmarks the study aims to point out strategic actions for Sweden regarding IWT. The data for this study was collected from IWT organizations, shippers and local administrations in the Netherlands. The results showed that main drivers for IWT are congestion relief, cost reduction and lower environmental impact. On the other hand, main barriers are slow pace of development, high investment costs and poor hinterland connectivity. For a successful modal shift in Sweden, it is crucial to prepare governmental support, a change in stakeholders’ mindset, decisive attitude to modal shift process and a strong long-term perspective

Lung Function and Risk of Type 2 Diabetes and Fatal and Nonfatal Major Coronary Heart Disease Events: Possible Associations With Inflammation

OBJECTIVE - We prospectively examined the relationship between lung function and risk of type-2 diabetes and fatal and nonfatal coronary heart disease (CHD) events and investigated the hypothesis that inflammation may underlie these associations. RESEARCH DESIGN AND METHODS - A prospective study of 4,434 men aged 40-59 years with no history of cardiovascular disease (CHD or stroke) or diabetes drawn from general practices in 24 British towns and followed up for 20 years. RESULTS - There were 680 major CHD events (276 fatal, 404 nonfatal) and 256 incident type 2 diabetes during the 20 years follow-up. Forced vital capacity (FVC) and forced expiratory volume in 1 s (FEY1) but not FEV1-to-FVC ratio were significantly and inversely associated with incident type 2 diabetes and fatal CHD events (not nonfatal events) after adjustment for age, potential confounders, and metabolic risk factors. The adjusted relative risk (RR) for type 2 diabetes (Quartile 1 vs. Quartile 4) were 1.59 (1.07-2.56) and 1.74 (1.16-2.61) for FVC and FEV1, respectively (P = 0.03 and P = 0.04 for trend). The corresponding RR for fatal CHD were 1.48 (1.00-2.21) and 1.81 (1.19-2.76) (P = 0.002 and P = 0.0003 for trend). Lung function was significantly and inversely associated with C-reactive protein and interleukin-6; the inverse associations with type 2 diabetes for FVC and FEV1 were attenuated after further adjustment for these factors (P = 0.14 and P = 0.11 for trend) but remained significant for fatal CHD (P = 0.03 and P = 0.01, respectively). CONCLUSIONS - Restrictive rather than obstructive impairment of lung function is associated with incident type 2 diabetes (and fatal CHD) with both associations partially explained by traditional and metabolic risk factors and inflammation