Biobutanol production by Clostridium acetobutylicum NCIMB 13357 in modified medium using date fruit as a carbon source

Abstract: Biofuel is an alternative energy that is conceived being the future energy source; it can be produced from natural and renewable agricultural raw materials by microbes. Clostridium acetobutylicum has the ability to ferment variety of compounds to produce solvents such as: Acetone, Butanol, and Ethanol (ABE). This study was designed to investigate the ability of C. acetobutylicum to produce biobutanol or any other possible solvents using date fruit as the organic carbon source under anaerobic condition. The fermentation process was carried out under the following conditions: initial pH 6 and 7, incubation temperatures 30 o C and 35 o C for 70 hours. The effect of date fruit concentrations on C. acetobutylicum was studied 10 -50 g/L. Results showed that 40 g/ L of date fruit under initial pH 7 at 35 o C were the optimum conditions for fermentation process to produce 5.31 g/ L and 8.42 g/ L as biobutanol and total solvents respectively. The yield and productivity of ABE was 0.52 and 0.12 g/(L.h), respectively

Enhanced Butanol Production by Clostridium acetobutylicum

The production of biobutanol was studied by the cultivation of Clostridium acetobutylicum NCIMB 13557 in P2 medium including date fruit as the sole substrate. The effect of P2 medium and the effect of different concentrations of date fruit ranging from 10 to 100 g/L on biobutanol production were investigated. Anaerobic batch culture was carried out at 35°C incubation temperature and pH 7.0 ± 0.2 for 72 h. Experimental results showed that the lowest yield of biobutanol and acetone-butanol-ethanol (ABE) was 0.32 and 0.35 gram per gram of carbohydrate consumed (g/g), respectively, when an initial date fruit concentration of 10 g/L was utilized. At this fruit date concentration a biobutanol production value of 1.56 g/L was obtained. On the other hand, the maximum yield of biobutanol (0.48 g/g) and ABE (0.63 g/g) was produced at 50 g/L date fruit concentration with a biobutanol production value as high as 11 g/L. However, when a higher initial date fruit concentration was used, biobutanol and ABE production decreased to reach the yield of 0.22 g/g and 0.35 g/g, respectively, where 100 g/L date fruit was used. Similar results also revealed that 10.03 g/L biobutanol was produced using 100 g/L date fruit