The effect of bacterial supplementation on black soldier fly larval growth and development

With a growing human population, food insecurity is becoming a worldwide problem. As the search for sustainable sources of protein continues, black soldier fly larvae come to the forefront as a partial solution. Full of proteins and fats, the larvae will consume most organic matter and rapidly develop into a usable form. Supplementing black soldier fly larvae with oleaginous microbes Arthrobacter AK19 and Rhodococcus rhodochrous increases their body size by 25%, potentially accelerates their development by one day, and increases their conversion efficiency. Supplementing with Bifidobacterium breve decreased body size, slowed development, and decreased conversion efficiency, underscoring the importance of selecting supplemental microbes and testing first on a small-scale. Promising results on the small-scale led to an industrial study, where similar results were also seen, resulting in greater optimization of this system

Bioinformatic Surveillance Leads to Discovery of Two Novel Putative Bunyaviruses Associated with Black Soldier Fly

The black soldier fly (Hermetia illucens, BSF) has emerged as an industrial insect of high promise because of its ability to convert organic waste into nutritious feedstock, making it an environmentally sustainable alternative protein source. As global interest rises, rearing efforts have also been upscaled, which is highly conducive to pathogen transmission. Viral epidemics have stifled mass-rearing efforts of other insects of economic importance, such as crickets, silkworms, and honeybees, but little is known about the viruses that associate with BSF. Although BSFs are thought to be unusually resistant to pathogens because of their expansive antimicrobial gene repertoire, surveillance techniques could be useful in identifying emerging pathogens and common BSF microbes. In this study, we used high-throughput sequencing data to survey BSF larvae and frass samples, and we identified two novel bunyavirus-like sequences. Our phylogenetic analysis grouped one in the family Nairoviridae and the other with two unclassified bunyaviruses. We describe these putative novel viruses as BSF Nairovirus-like 1 and BSF uncharacterized bunyavirus-like 1. We identified candidate segments for the full BSF Nairovirus-like 1 genome using a technique based on transcript co-occurrence and only a partial genome for BSF uncharacterized bunyavirus-like 1. These results emphasize the value of routine BSF colony surveillance and add to the number of viruses associated with BSF