A nation-wide census for future conservation actions

Citizen science is a type of research which could be crucial for wildlife monitoring in the developing countries due to low funding opportunities and trained specialists. In the Republic of Moldova, this concept is not fully understood and currently under-developed. The citizen science will bring new data in ornithological research, which is still in its early stages, due to the few ornithologists involved in scientific research. Thus, there is poor data and knowledge regarding occurrence and distribution of wild bird species across the country, which makes difficult to implement conservation strategies in order to conserve our bird fauna. White stork (Ciconia ciconia) is a common bird species which breeds near human settlements and is easily identifiable. This aspect makes the species to be a very good target for citizen science monitoring. The number of White stork nests in Republic of Moldova was estimated to be around 574 in 1984 and continued to decrease since then. During 1990-2010 the official numbers pointed to 180 nests, while the breeding stork population was considered to be around 400-600 breeding pairs. During June – August 2017 the Society for the Birds and Nature Protection (SPPN) carried out a national census when 283 nests were recorded. The nests were registered on a digital map with the help of citizen volunteers. The concept and the procedure were a novelty for Republic of Moldova, as the observations were uploaded using an online survey which provided additional information about the nests (type of support, condition, number of offspring). The impact among citizens was high: 90 volunteers participated in the census across the country. Most of the nests were installed on concrete pillars (57.24%), followed by wooden pillars (17.67%), 15.19% were found on trees, while 9.19% were installed on other types of support (towers, monuments and barns). Only one nest was found on a house. Comparative with the previous monitoring program, from 1984, the White stork population from Republic of Moldova changed the breeding behavior, using the electric poles in a much higher percent. These changes in species ecology should be included in the species conservation strategy for the next years. Out of the total number of 283 nests, 83% were occupied in the 2017 breeding season, with a breeding success of 1.51 offspring per nest during the study period. The 2017 White stork nest census in Republic Moldova proved to be an efficient tool for field data collection. Moreover, the information improved significantly the knowledge regarding the breeding status of White stork and raised awareness about the ecology of this species. For the Republic of Moldova scientific community, which is young and still developing, the census represents the first step for future conservation projects and a successful start of citizen science methods.peerReviewe

The distribution of Great Grey Shrike in Republic of Moldova

The Great Grey Shrike (Lanius excubitor) is the largest species from the Laniidae family, with a wide distribution in the Northern Hemisphere, across Europe, Asia and North America. Because the species occupies a large area, the data about the spring and autumn migration and wintering areas of the whole population is poor documented. The study area covers the territory of the Republic of Moldova (33 843.5 km2). Here, the Great Grey Shrike was known as a wintering visitor, being recorded during the late autumn up to early spring. The species was not considered to breed until now in the Republic of Moldova, therefore it was not listed in the Bird Atlas. Even today, the Great Grey shrike is a poorly studied species in our country and there is no up-to-date information except the actual study. The first breeding recorded was in 2012 in the north region of the country. After that, we started a monitoring program in order to check the species’ status. The aim of the current study was to assess the distribution of the Great Grey Shrike in the Republic of Moldova and to analyze the breeding and wintering population. The study was conducted from 2014 to 2017. During two breeding seasons (2014 and 2015) we carried out point counts with a minimum distance of 400 m between them and 5 minutes of observation in each location.. The observation points were randomly selected across the study area using Hawths Tool for Arc GIS v.9.3. In 2014 we registered 20 breeding pairs out of 470 observations and for 2015 we counted 31 breeding pairs out of 631 observation points. The density of the Great Grey Shrikes in Republic of Moldova estimated, for the research period, to 0.2-0.4 breeding pairs/square km. According to our study, its breeding range is increasing (occupying 75% of the country), extending to south, contrary to previous conclusions which stated that the population is concentrated in north. The GLM analysis showed that the great grey shrike breeds in areas with higher altitudes (a mean of 174 m), low level of precipitation in November and with low average temperatures (all P< 0.05). To estimate the wintering population, we used the line transect method. Monthly, we conducted 9 transects about 40 km long each (360 km per month), during November - February, for two seasons, 2015 – 2016 and 2016 –2017. The method consists in driving a car at slow speed (up to 40 km/h) with very short stops, if is necessary. The distribution across the country was uneven, 42 were observed in northern area, 37 in the central one and only 5 in south. The numbers of individuals in the first wintering season was 46, while in the second there were 38 individuals counted in Republic of Moldova. According to Manly’s selectivity index the Great Grey Shrike select during winter season orchards (Wi= 1,6) and grasslands (Wi= 3.0). The data collected during the winter season shows that birds tend to keep their breeding spots, being recorded near them during this harsh season.peerReviewe

Weather Influence on Native and Alien Mantis Dynamics and Their Abundance in the Current Climate Change Conditions

Humans have traded and transported alien species for millennia, both with and without intention to spread them to new areas. Consistent knowledge of their ecology will allow decision makers to take suitable conservation actions, with the aim of avoiding threatening native species. Praying mantids (Mantodea) are predatory insects with a high impact on local invertebrates’ fauna. An alien mantis species (Hierodula tenuidentata) could create a disequilibrium in both the local ecosystem and in autochthonous mantid species (Mantis religiosa) if it can adapt to the local ecological conditions. Through this study, we reveal that the number of Hierodula tenuidentata individuals from an Eastern European Natura 2000 site was 7.6 times higher than the number of Mantis religiosa suggesting a higher density of the allochthonous species in the study area. According to a GLM analysis, the population of Mantis religiosa, measured from August to the end of October, declines more rapidly and is negatively influenced by the number of days from the first day of the year, while the population of Hierodula tenuidentata is influenced by local weather factors. This is the first study which analyzes the influence of local weather factors (namely air temperature, precipitation, daily atmospheric pressure, daily wind direction and speed, daily cloud cover, sunshine duration and number of days from the first day of the year) on the abundance dynamic of mantises in order to understand their ecology in the current climate change influence

Weather Influence on Native and Alien Mantis Dynamics and Their Abundance in the Current Climate Change Conditions

Humans have traded and transported alien species for millennia, both with and without intention to spread them to new areas. Consistent knowledge of their ecology will allow decision makers to take suitable conservation actions, with the aim of avoiding threatening native species. Praying mantids (Mantodea) are predatory insects with a high impact on local invertebrates’ fauna. An alien mantis species (Hierodula tenuidentata) could create a disequilibrium in both the local ecosystem and in autochthonous mantid species (Mantis religiosa) if it can adapt to the local ecological conditions. Through this study, we reveal that the number of Hierodula tenuidentata individuals from an Eastern European Natura 2000 site was 7.6 times higher than the number of Mantis religiosa suggesting a higher density of the allochthonous species in the study area. According to a GLM analysis, the population of Mantis religiosa, measured from August to the end of October, declines more rapidly and is negatively influenced by the number of days from the first day of the year, while the population of Hierodula tenuidentata is influenced by local weather factors. This is the first study which analyzes the influence of local weather factors (namely air temperature, precipitation, daily atmospheric pressure, daily wind direction and speed, daily cloud cover, sunshine duration and number of days from the first day of the year) on the abundance dynamic of mantises in order to understand their ecology in the current climate change influence

Serological Survey of Mosquito-Borne Arboviruses in Wild Birds from Important Migratory Hotspots in Romania

In the context of climate change, globalization, and enhanced human traveling, arboviruses continue to represent a threat to public health. West Nile and Usutu viruses are mosquito-borne viruses belonging to the Flaviviridae family (Flavivirus genus) and members of the Japanese encephalitis virus serocomplex. Included in the Togaviridae family (Alphavirus genus), the Sindbis virus is also vectored by mosquitoes. In the present study, we aimed to analyze the presence of antibodies concerning the abovementioned viruses in migratory and resident birds in the South-Eastern region of Romania, as avian hosts represent the main reservoir for human infection. Blood samples were collected from wild birds between May 2018 and October 2019 in nine locations from three counties. All the samples were serologically tested by ELISA and a serum neutralization test. Overall, a seroprevalence of 8.72% was registered for the West Nile virus, 2.71% for the Usutu virus, and 0% for the Sindbis virus. To our best knowledge, this is the first large-scale comprehensive study to assess the West Nile virus seropositivity in wild birds and the first serological confirmation of the Usutu virus in wild birds in Romania. Moreover, this is the only follow-up study reviewing the current seroprevalence of the Sindbis virus in Romania since 1975