_Trichoderma pseudokoningii_ Rifai isolation from Egyptian immunocompromised cattle with _Mycobacterium bovis_ infection

Recently, _Trichoderma_ species have emerged as potent fungal pathogens in immunocompromised humans. We report the first three cases of _Trichoderma pseudokoningii_ Rifai pulmonary infection in the Egyptian tuberculous dairy cattle with _Mycobacterium bovis_, from the heart of a generalized bovine TB in a cow over 5 years old, a mediastinal lymph node of pulmonary TB in a cow of 3 years old, and a lung of mixed pulmonary and digestive BTB in a cow of 4 years old. We have also developed a pathogenisity test technique for _Trichoderma pseudokoningii_ Rifai infection in 3 G. pigs by intraperitoneal injection of 2 G. pigs with mixed infection of _Mycobacterium bovis_ and _Trichoderma pseudokoningii_ Rifai; death of both animals 14 days, thereafter, and by injection of 1 G. pig with single infection of _Trichoderma pseudokoningii_ Rifai; death of animal 21 days, thereafter. We did not report any animal case along review of literature

Extracting Synonyms from Bilingual Dictionaries

We present our progress in developing a novel algorithm to extract synonyms from bilingual dictionaries. Identification and usage of synonyms play a significant role in improving the performance of information access applications. The idea is to construct a translation graph from translation pairs, then to extract and consolidate cyclic paths to form bilingual sets of synonyms. The initial evaluation of this algorithm illustrates promising results in extracting Arabic-English bilingual synonyms. In the evaluation, we first converted the synsets in the Arabic WordNet into translation pairs (i.e., losing word-sense memberships). Next, we applied our algorithm to rebuild these synsets. We compared the original and extracted synsets obtaining an F-Measure of 82.3% and 82.1% for Arabic and English synsets extraction, respectively.Comment: In Proceedings - 11th International Global Wordnet Conference (GWC2021). Global Wordnet Association (2021

Toxoplasma gondii: Prolonged in-vitro maintenance of virulent tachyzoites in fluid media at low temperatures

Background: Prolonged maintenance of infective Toxoplasma gondii tachyzoites (T.g.T.) is an important subject for research purposes. This study aimed to evaluate four serum free fluid media for prolonged in vitro maintenance of T.g.T.Methods: The four fluid media Phosphate buffered saline (PBS) pH 7.2 and Roswell Park Memorial Institute (RPMI-1640) with or without 3% fetal bovine serum (FBS) were evaluated for maintenance of virulent T.g.T. The four media were tested after incubation at three different temperature degrees in the darkness.Results: Prolonged maintenance period for infective T.g.T. was recorded especially in the absence of FBS supplement. RPMI without FBS was able to maintain infective T.g.T. for 16 days post incubation (dpi) at refrigerator temperature. This period decreased to 10 dpi and 6 dpi after incubation in the same media at 18–22 oC and 37 oC, respectively. Cultivation of T.g.T. in RPMI supplemented with 3% FBS and in PBS proved to maintain infective T.g.T. for 14 dpi at refrigerator temperature, and for 9 and 5 dpi when the two media were incubated at 18–22 oC and 37 oC, respectively. Shorter periods for keeping the T.g.T. infectivity were recorded using PBS supplemented with 3% FBS under all tested temperature conditions.Conclusion: This method allows economic long-lasting maintenance of tachyzoites for 16th dpi in RBMI that can be reactivated by reinoculation in mice.Keywords:  Toxoplasma gondii, Tachyzoites, Fluid media, Temperatur

Oral health-related interdisciplinary practices among healthcare professionals in Saudi Arabia: Does integrated care exist?

Background There is a bidirectional relation between oral and general health, therefore collaboration between healthcare providers is needed. This study investigated current interdisciplinary practices (IDP) and the associated factors among healthcare professionals in Saudi Arabia. Methods A cross-sectional study was conducted in the Eastern Saudi Arabia recruiting four groups of health professionals (nurses, physicians, pediatricians and Ear-Nose and Throat (ENT) specialists). A validated, self-administered questionnaire was distributed online and shared through social media platforms. The questionnaire explored predisposing factors (demographics) and facilitating factors (knowledge, attitudes, attendance of oral health training and source of knowledge) associated with IDP. Results A total of 1398 health professionals were recruited. Participants showed fair oral health knowledge (7.1 ± 2.1) and attitudes (22.2 ± 3). Three-fourths (74.6%) reported always providing oral health education (OHE) to their patients, more than half (59.6%) reported always conducting an oral health screening (OHS), two-thirds (66.7%) reported responding to patients’ questions about oral health or conditions and 58.7% reported referring patients to dentists. Pediatricians and physicians had greater odds of IDP compared to other health professionals. Source of oral health knowledge (Ministry of Health (MOH) and formal education) was significantly associated with increased odds of IDP. Participants with good oral health knowledge had greater odds of responding to patients’ oral health question as well as have more referral practices. Conclusion The results reveal a discrepancy between participants' IDP, knowledge, and attitudes. Incorporating dental component to medical curricula, continuous education and training programs targeting health professionals through Ministry of Health should be considered.publishedVersio

Determinations of System Justification versus Psychological Reactance Consumer Behaviours in Taboo Markets

Purpose: This research aims to understand the determinants of consumers' behaviour and motivations to buy taboo items online. Two theoretical lenses, theories of psychological reactance and system justification, are invoked to frame the role of online shopping in shaping consumer behaviour in taboo markets. Design/methodology/approach: A naturalistic inquiry paradigm was used to test a sample of 34 Saudi women who were buying taboo products online. Findings: The determinant of such behaviour is based on differences in understanding the ritual restrictions between people, their society and their country. The four principal attitudes towards restrictions are justifying, accepting, rejecting and reacting. These attitudes frame five motivations: satisfying the restriction, to be unique, but aligned with social norms; breaking social norms; aligning one's self-image to liberal societies; and joy in challenging legal restrictions. The motives for online shopping are justification/utilitarian, to accommodate other restrictions in going to local markets; and reactance hedonic, to break restrictions. These motivations create seven different patterns of online shopping behaviour. Originality/value: This research contributes to the literature by presenting an alternative perspective on online shopping motivations for taboo products. Furthermore, this research calls for a new socio-psychological theory for understanding the role of technology in influencing consumer behaviour in restrictive societies

INFLUENCE OF SOME BIOSTIMULANTS AND CHEMICAL FERTILIZERS ON GROWTH, SEED YIELD, CHEMICAL CONSTITUENTS, OIL PRODUCTIVITY AND FIXED OIL CONTENT OF CHIA (SALVIA HISPANICA L.) PLANT UNDER ASWAN CONDITIONS

This study was carried out at a private farm in AL-Radisiya city, Markaz Edfu, Aswan Governorate, Egypt on the banks of Nile River and Experimental Laboratory of Horticulture Department, Faculty of Agriculture, Benha University, EL-Qaloubia Governorate, Egypt during the two successive seasons of 2018/2019 and 2019/2020 on chia (Salvia hispanica L.) plant. A study was initiated through various biostimulant and chemical fertilizer aiming to evaluate effectiveness of arbuscular mycorrhiza fungi (AMF) or seaweed extract and/or humic acid as well as chemical fertilizer (N.P and K) and the combination of them on vegetative growth, seed yield, chemical constituent's, fixed oil productivity and fixed oil composition of chia (Salvia hispanica L.) plant. Plants sprayed by biostimulants gave the significantly highest mean values for all of studied characteristics mentioned above, particularly T6: the combined of mycorrhiza, humic acid, seaweed and recommended dose of NPK followed by T5: recommended dose of NPK (350:200:100 kg/feddan) in the first and second seasons. Meanwhile, T4: seaweed (1 ml/l) ranked the third values in parameters mentioned before in this concern. Whereas, the richest fixed oil percentage was scored by T6, followed descendingly by T5 in the 1st and 2nd seasons. Chromatography analysis of chia fixed oil revealed the identification of four components. i.e. palmitic acid, oleic acid, linoleic acid and α-linolenic acid. The main component was α-linolenic acid (43.34 to 53.28%). It can be concluded that T6 was the best for improving growth, seed yield, fixed oil productivity, chemical constituents and fixed oil components of Salvia hispanica L. plant

Oral health-related interdisciplinary practices among healthcare professionals in Saudi Arabia: Does integrated care exist?

Background: There is a bidirectional relation between oral and general health, therefore collaboration between healthcare providers is needed. This study investigated current interdisciplinary practices (IDP) and the associated factors among healthcare professionals in Saudi Arabia.Methods: A cross-sectional study was conducted in the Eastern Saudi Arabia recruiting four groups of health professionals (nurses, physicians, pediatricians and Ear-Nose and Throat (ENT) specialists). A validated, self-administered questionnaire was distributed online and shared through social media platforms. The questionnaire explored predisposing factors (demographics) and facilitating factors (knowledge, attitudes, attendance of oral health training and source of knowledge) associated with IDP.Results: A total of 1398 health professionals were recruited. Participants showed fair oral health knowledge (7.1 ± 2.1) and attitudes (22.2 ± 3). Three-fourths (74.6%) reported always providing oral health education (OHE) to their patients, more than half (59.6%) reported always conducting an oral health screening (OHS), two-thirds (66.7%) reported responding to patients' questions about oral health or conditions and 58.7% reported referring patients to dentists. Pediatricians and physicians had greater odds of IDP compared to other health professionals. Source of oral health knowledge (Ministry of Health (MOH) and formal education) was significantly associated with increased odds of IDP. Participants with good oral health knowledge had greater odds of responding to patients' oral health question as well as have more referral practices.Conclusion: The results reveal a discrepancy between participants' IDP, knowledge, and attitudes. Incorporating dental component to medical curricula, continuous education and training programs targeting health professionals through Ministry of Health should be considered.</p

Diagnostic performance of loop-mediated isothermal amplification (LAMP) and Ultra-sensitive PCR in diagnosis of malaria in western Saudi Arabia

Malaria diagnosis continues to be one of the most important steps in the cycle of control specially in endemic countries with low parasitic load infections. Loop-mediated isothermal amplification (LAMP) and ultrasensitive PCR (Us-PCR) are two promising candidates for malaria diagnosis. A cross sectional study performed at King Faisal Hospital, Taif KSA involved patients suffering from signs and symptoms suggesting of malaria, 35 blood samples diagnosed by Nested Multiplex PCR as a reference method (13 P. falciparum, 17 P. vivax, 3 mixed P. falciparum and P. vivax) plus two negative controls were selected to be included in this study to analyse the performance of two LAMP methods (LAMP OptiGene® and LAMP WarmStart®) and two ultrasensitive PCRs (Us-PCR TARE-2 and Us-PCR Var-ATS). LAMP OptiGene® and LAMP WarmStart® performances were identical and better than the performance of Us PCR TARE 2 and Us-PCR var-ATS for P. falciparum, achieving 93.75% sensitivity, 100% specificity and 97.17% accuracy versus 87.5% sensitivity, 100% specificity and 94.29% accuracy for the Us PCR TARE 2 and 81.25% sensitivity, 94.74% specificity and 88.57% accuracy for the Us PCR var-ATS respectively. In P. vivax diagnosis LAMP OptiGene® performed excellently with 100% sensitivity, specificity, and accuracy while LAMP WarmStart® and Us-PCR Cox1 achieved 100% sensitivity, specificity 93.33% and 97.14% accuracy. The study results highlighted the benefits of using LAMP techniques for field diagnosis of malaria in different settings where the need for a more sensitive and reliable molecular tool is mandatory but at the same time removing the high cost, long turnaround time and the need of highly specialized trained technicians to perform more sophisticated molecular techniques.The authors would like to acknowledge The Deanship of Scientific Research, Taif University, Taif, Saudi Arabia for funding of the resent research.S

Ischemia-modified albumin relation to glycemic state, neuropathy, and retinopathy in patients with type2 Diabetes mellitus

Background: The incidence of microvascular complications is rising although there is improvement in glycemic control, dyslipidemia, and hypertension treatment. Early identification of patients with a high risk of developing vascular complications helps in their prevention. There is a strong need for biomarkers for use in the early detection of microvascular complications. Ischemia-modified albumin (IMA) is formed when ischemia of the hypoxic tissue induces modification of circulating albumin. IMA is a sensitive marker of tissue hypoxia and oxidative stress. Objectives: to evaluate the relationship of IMA to glycemic state and its ability to predict microvascular complications of diabetic neuropathy (DN) and diabetic retinopathy (DR) in type 2 DM patients. Patients and methods:100 Participants were divided into three groups: Group A: (n=35) complicated type 2 diabetic patients (with neuropathy, retinopathy, or both). GroupB: (n=35) non-complicated type 2 diabetic patients, GroupC: (n=30) control group (healthy individuals). Results: Using the ROC curve of IMA in predicting diabetic neuropathy and retinopathy, showed high sensitivity, the area under the curve (AUC) is 0.948 (95% CI 0.885:0.983), p 95.7 U/ml (sensitivity =100%, specificity =95.7%, PPV =74.3%, and NPP =100%), ROC curve of IMA in predicting diabetic retinopathy the AUC is 0.960 (95% CI 0.900:0.989), p 110.5 U/ml (sensitivity =100%, specificity =94.4%, PPV =87.9%, and NPP=100%). Conclusion: Ischemia-modified albumin levels were significantly higher in patients with DN and DR