Application of miniature heart rate data loggers for use in large free-moving decapod crustaceans: Method development and validation

Cardiovascular responses of decapod crustaceans to environmental challenges have received extensive attention. However, nearly all of these studies have been restricted to lab-based experiments; here we describe a methodology that will enable measurement of heart rate (HR) in free-moving decapods in the field. Data storage tag heart rate and temperature loggers (DST micro-HRT; Star-Oddi) were used to record electrocardiograms (ECG) and HR in large decapod crustaceans. These loggers were originally designed for use in vertebrates and must be surgically implanted in the body cavity near the heart in order to function. We adapted these loggers for external use in large decapod crustaceans. The method involved abrading the carapace directly above the heart and placing the electrodes of the logger directly on top of the dermal tissue. The loggerwas then secured in place with periphery wax. This method negated some of the more intricate operations used for vertebrates. The rapid setup time of approximately 5 min suggested that animals could be easily instrumented in the field and without the use of anesthetic. The logger was calibrated by simultaneously measuring theHRchanges of aWest Indianspider crabMith

As I see it: On the neglected cold side of climate change and what it means to fish

Over the past decade nearly all of the research on the effects of climate change on fish has focused on the effects of warmer water temperatures. Yet, it is expected that temperature variability will also increase, resulting in more frequent incidences of rapid decreases in water temperatures (i.e. cold shock). Cold shock events have caused large-scale fish mortalities, and sublethal impacts are also known to occur but are less well documented. We argue that cold shock will become an important selective force in climate change scenarios. There is a rich history of research on cold shock in the context of industrial cooling effluents and aquaculture, providing a foundation upon which to develop and extend future work on cold shock and climate change. To understand the diverse effects climate change may have on fish populations, future research needs to expand beyond the projected increases in water temperatures to include consideration of variability in temperature and the potential for cold shock

An experimental evaluation of the role of the stress axis in mediating predator-prey interactions in wild marine fish

The hypothalamic-pituitary-interrenal (HPI) axis, through corticosteroid secretion, is an integral mechanism regulating internal homeostasis when vertebrates are faced with a stressor. However, continued HPI-axis stimulation can produce homeostatic overload, where corticosteroids are detrimental to organismal function. This overload condition may play an important role in mediating predator-prey interactions, because chronically/previously stressed animals may have higher rates of predator-induced mortality. However, the mechanism(s) underlying this observation are unknown. Using fish as models, we hypothesized that chronic stress would increase predation susceptibility owing to a poor physiological state (e.g. homeostatic overload) with corresponding sub-optimal changes in predator-avoidance behaviour. As cortisol is also required in low quantities to help regulate basic metabolic functions in fish, we expected that a glucocorticoid receptor antagonist (GR; e.g. homeostatic failure) may produce similar effects. Schoolmaster snapper (Lutjanus apodus) were given intraperitoneal implants of cocoa butter impregnated with nothing (sham; 5 ml/kg body weight (BW)), cortisol (50 mg/kg BW) or the GR antagonist RU486 (100 mg/kg BW). At 24-h post-implantation, fish were tethered to the seafloor and observed for behavioural metrics associated with predation. Blood samples were collected from a subset of fish to assess the physiological consequences of the implants. Cortisol- and RU486-implanted fish both had significantly higher plasma cortisol concentrations than sham fish, with blood glucose and plasma urea being elevated only in the former. Furth

Cortisol modulates metabolism and energy mobilization in wild-caught pumpkinseed (Lepomis gibbosus)

Acute elevation of cortisol via activation of the hypothalamic-pituitary-interrenal (HPI) axis aids the fish in dealing with a stressor. However, chronic elevation of cortisol has detrimental effects and has been studied extensively in lab settings. However, data pertaining to wild teleosts are lacking. Here, we characterized the metabolic consequences of prolonged cortisol elevation (96 h) in wild-caught pumpkinseed (Lepomis gibbosus). Pumpkinseed were implanted with cocoa butter alone (sham) or containing cortisol (25 mg kg−1 body weight), and at 24, 48, 72, and 96 h, tissue samples were collected, whole-body ammonia excretion was determined, and whole-organism metabolism was assessed using intermittent

Influence of supraphysiological cortisol manipulation on predator avoidance behaviors and physiological responses to a predation threat in a wild marine teleost fish

The stress axis in teleost fish attempts to maintain internal homeostasis in the face of allostatic loading. However, stress axis induction has been associated with a higher predation rate in fish. To date, the physiological and behavioral factors associated with this outcome are poorly understood. The purpose of the present study was to investigate the impact of experimental cortisol elevation on anti-predator behavior and physiological responses to predator presence. We hypothesized that semi-chronic cor

Physiological Basis of Climate Change Impacts on North American Inland Fishes

Global climate change is altering freshwater ecosystems and affecting fish populations and communities. Underpinning changes in fish distribution and assemblage-level responses to climate change are individual-level physiological constraints. In this review, we synthesize the mechanistic effects of climate change on neuroendocrine, cardiorespiratory, immune, osmoregulatory, and reproductive systems of freshwater and diadromous fishes. Observed climate change effects on physiological systems are varied and numerous, including exceedance of critical thermal tolerances, decreased cardiorespiratory performance, compromised immune function, and altered patterns of individual reproductive investment. However, effects vary widely among and within species because of species, population, and even sex-specific differences in sensitivity and resilience and because of habitat-specific variation in the magnitude of climate-related environmental change. Research on the interactive effects of climate change with other environmental stressors across a broader range of fish diversity is needed to further our understanding of climate change effects on fish physiology

Emerging threats and persistent conservation challenges for freshwater biodiversity

In the 12 years since Dudgeon et al. (2006) reviewed major pressures on freshwater ecosystems, the biodiversity crisis in the world's lakes, reservoirs, rivers, streams and wetlands has deepened. While lakes, reservoirs and rivers cover only 2.3% of the Earth's surface, these ecosystems host at least 9.5% of the Earth's described animal species. Furthermore, using the World Wide Fund for Nature's Livin