Artificial Water Troughs Use by the Mountain Ungulate Ovis gmelini ophion (Cyprus Mouflon) at Pafos Forest

For large herbivores inhabiting arid/semi-arid environments, water can be a limiting resource affecting their distribution and abundance for periods when water requirements are not met via forage. The Cyprus mouflon (Ovis gmelini ophion) is such a species, which is endemic to the mountain habitats of Cyprus. Recognizing water scarcity to be a major pressure to the mouflon, and with global warming projected to intensify hot and dry periods in the region, the Game and Fauna Service has been maintaining a network of locally designed watering troughs in Pafos Forest—the mouflon’s stronghold—since 1997. This study describes the mouflon’s use of the water troughs and examines whether visitation rates differed at the daily or weekly scale in response to environmental, climatic or anthropogenic parameters. Using camera traps, ten troughs were monitored from September 2017 to March 2018 (1,065 days; range 29–164 days per trough). Mouflon were detected at seven troughs (mean herd size 1.5 ± 1.2) during 373 independent detections (≥30 min interval between photographs), with visits peaking during late morning and midday hours. Generalized mixed-effect models showed mouflon visiting water troughs more frequently during hotter days, regardless of recent precipitation. Visits were also more frequent at water troughs located close to tar roads. Moreover, there was no evidence of mouflon avoiding water troughs used by predators (red foxes, feral dogs) at either daily or weekly scale, or during hunting days. The study supports the value of artificial water troughs for mediating, partially at least, the effects of climate change on mountain ungulates such as the Cyprus mouflon. Additional studies are proposed that will examine both mouflon drinking patterns across all seasons and ways of improving the effectiveness of the current water trough grid

High genetic diversity and significant population structure in Cedrus brevifolia Henry, a narrow endemic Mediterranean tree from Cyprus

Endemic island plant species with a narrow distribution are often, but not always, linked to low genetic variation within populations and a lack of differentiation among populations. Cedrus brevifolia is a narrow endemic island tree species of Cyprus. Its range is restricted to a single forest, divided into five neighbouring sites. This study, using biparentally inherited nuclear microsatellites and paternally inherited plastid (chloroplast) microsatellites, assessed the genetic variation of C. brevifolia within its sole population and the level of genetic differentiation among formed sites. The results from both markers showed high diversity (nuclear HT = 0. 70; plastid HT = 0. 93), strongly suggesting that the species did not experience severe bottleneck events or extensive genetic drift. Besides, the maintenance of a high genetic diversity in C. brevifolia may suggest that it originates from a widespread congener species. Significant genetic differentiation at nuclear (GST = 0. 052) and plastid (GST = 0. 119) markers was found among the formed sites. Remarkably, the relatively high genetic differentiation found at plastid markers was comparable to values observed in two widespread congener cedar species. The genetic differentiation probably occurred due to fragmentation of a previously uniform population. This would lead to the shaping of different genetic groups (Bayesian analysis) and to significant population substructure. Furthermore, significant values observed for both isolation by distance and large-scale spatial genetic structure could indicate ineffective gene flow among sites and the early geographical isolation of the more isolated sites from the core population. © 2011 The Author(s)

An approach to genetic resources conservation of peripheral isolated plant populations: the case of an island narrow endemic species

This study reinforces the argument that forest genetic resources for peripheral isolated plant populations (PIPP) need to come under rationalistic assessment, for developing and proposing conservation strategies of forest genetic resources. Cedrus brevifolia is a narrow endemic island tree occurring in a sole population. Using biparentally and paternally inherited microsatellites, the genetic contribution and genetic variation patterns within sites (subpopulations) and within plantations of this species were assessed. The C. brevifolia population recorded uneven partition of genetic diversity at the site and at local scales; mirroring the demography and genetic evolutionary factors that occurred in long-term at the intra-species level. Results from the plantations imply that screening and rejection of unsuitable seedlings before plantation establishment did not influence the genetic divergence of planted trees from the general genetic pool of natural population. Based on these outcomes, numerous conservation measures are proposed, towards maintaining the species’ genetic resources. These measures need to ensure the dynamic processes within stands. Thus, this study argues that for PIPP which recorded high genetic diversity and non-uniform distribution of genetic viability (complex genetic patterns), the several small conservation units seem to be the most rationalistic conservation units. In such case, forest genetic micro-reserves (GMR) can be enacted, where on-the-point dynamic in situ conservation activities can be adopted, while the GMR could be used for ex situ conservation purposes. In conclusion, in PIPP the maintenance of genetic diversity should be regarded as one of the main prerequisites for sustainable management and long-term survival of the species

Chemical Characterization, Antioxidant and Antimicrobial Properties of Different Types of Tissue of Cedrus brevifolia Henry Extracts

This study aimed to determine the chemical composition of different types of tissue of Cedrus brevifolia Henry (Pinaceae) methanolic extracts, namely needles, twigs, branches, and bark. Cedrus brevifolia is a narrow endemic coniferous tree species of Cyprus, growing in a sole population in the mountainous area of Paphos Forest. Chemical analysis of the extracts was performed using liquid chromatography combined with time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The majority of the 36 compounds tentatively identified belonged to the flavonoids family. The extract of needles was the richest extract in terms of secondary metabolites. The extracts were studied for their antioxidant activity using the DPPH free radical scavenging assay. Additionally, the antibacterial activity was evaluated by determining both the minimum inhibitory concentration and the minimum bactericidal concentration against Staphylococcus aureus and Escherichia coli. All extracts demonstrated antioxidant property, while bark gave the highest antioxidant capacity (IC50 value of 0.011 mg/mL) compared to the other tissues. Antibacterial activity was observed against both types of bacteria, with the extract of branches presenting the strongest activity against S. aureus (MIC, 0.097 mg/mL and MBC, 0.195 mg/mL). This is the first time that extracts of needles, twigs, branches, and bark of C. brevifolia are compared regarding their chemical composition as well as their antimicrobial and antioxidant properties

Paper Mapping Asia plants: Current status on floristic information in Southwest Asia

Mapping Asia Plants (MAP) is a comprehensive project that aims to build a detailed infrastructure for integrating Asian plant distribution data a global-scale array of knowledge for plant biodiversity conservation. Here, we provide a brief historical review of botanical research in Southwest Asia an understudied botanical region with high conservation priority. Nineteen countries were included in this study (from west to east): Turkey, Cyprus, Palestine, Israel, Jordan, Saudi Arabia, Lebanon, Syria, Iraq, Georgia, Yemen, Armenia, Iran, Azerbaijan, Kuwait, Bahrain, Qatar, United Arab Emirates, and Oman. We reviewed 132 resources comprising 125 Floras and Checklists, of which we describe in some detail at least one of the most important Floras or Checklists for each country. Complete and published national Floras exist for 13 countries; three countries (Jordan, Israel and Bahrain) do not have a Flora but have annotated Checklists, and national Floras are at different stages of completion for Iran, Iraq and Georgia. Where present, online resources are also given for references. We found major gaps in species concepts and taxonomic classification systems, and that many up-to-date Flora revisions remained unresolved, i.e. taxon ranks and species concepts varied among different countries, different systems were adopted or followed in the taxonomic treatments in the Floras and Checklists, and some of the current Floras are out of date. Floras are the first necessary step for many fields, including evolutionary biology, ecology, biogeography, and systematics, as well as environmental research and conservation of biodiversity at national and international levels. Here, we provide the progress updates on the main published floristic works of Southwest Asia, which continue to serve as references for the Flora of Southwest Asia, and will be the foundation of the MAP project. (C) 2020 The Author(s). Published by Elsevier B.V

Forest Genetics Research in the Mediterranean Basin: Bibliometric Analysis, Knowledge Gaps, and Perspectives

International audiencePurpose of Review Recognizing that in the context of global change, tree genetic diversity represents a crucial resource for future forest adaptation, we review and highlight the major forest genetics research achievements of the past decades in biodiversity-rich countries of the Mediterranean region. For this, we conducted a bibliometric analysis of the scientific literature spanning the past thirty years (1991-2020). Putting together the representative regionwide expertise of our co-authorship, we propose research perspectives for the next decade. Recent Findings Forest genetics research in Mediterranean countries is organized into three different scientific domains of unequal importance. The domain "Population diversity and Differentiation" related to over 62% of all publications of the period, the domain "Environmental conditions, growth and stress response" to almost 23%, and the domain "Phylogeography" to almost 15%. Citation rate was trending the opposite way, indicating a strong and sustained interest in phylogeography and a rising interest for genetics research related to climate change and drought resistance. The share of publications from Asia and Africa to the total within the Mediterranean increased significantly during the 30-year period analyzed, reaching just below 30% during the last decade. Describing poorly known species and populations, including marginal populations, using the full potential of genomic methods, testing adaptation in common gardens, and modeling adaptive capacity to build reliable scenarios for forest management remain strategic research priorities. Delineating areas of high and low genetic diversity, for conservation and restoration, respectively, is needed. Joining forces between forest management and forest research, sharing data, experience, and knowledge within and among countries will have to progress significantly, e.g., to assess the potential of Mediterranean genetic resources as assisted migration material worldwide. Introductory quote: Let us collect with care the facts we can observe, let us consult experience wherever we can, and when this experience is inaccessible to us, let us assemble all the inductions which observation of facts analogous to those which escape us can furnish and let us assert nothing categorically; in this way, we shall be able little by little to discover the causes of a multitude of natural phenomena, and, perhaps, even of phenomena which seem the most incomprehensible... J.B. de Lamarck (Philosophie zoologique, 1809), cited by O. Langlet (1971)