Timokinon uygulamasının ratların pankreas dokusunda somatostatin salgısı üzerine etkileri]

In this study, it was aimed to investigate the effects of thymoquinone (TQ), which is the bioactive phytochemical constituent of the seeds oil of Nigella sativa, on somatostatin secretion in the rat pancreatic tissue by immunohistochemical method. Animals (n=30) were divided into 3 groups as follows: control, sham and thymoquinone. While Thymoquinone group received 8 mg/kg of TQ which injected for 14 days, only isotonic saline was injected to the sham group for same time. The control group received nothing. Crossman's triple staining was applied to tissue sections to examine histology. Streptavidin-Biotin-Peroxidase Complex method was used to investigate somatostatin immunoreactivity in the pancreatic tissue. Specific somatostatin immunoreactivities were observed in endocrine cells (islets of Langerhans) in all groups. It was determined that somatostatin secretion increased in the thymoquinone group compared to control and sham. In conclusion, thymoquinone administration was concluded to increase the secretion of somatostatin that is known to regulate certain hormones such as growth hormone, insulin, glucagon, secretin and gastrin.Namik Kemal University Commission for the Scientific Research (NKUBAP)Namik Kemal University [NKUBAP.00.M9.AR.14.02]This study was supported by Namik Kemal University Commission for the Scientific Research (NKUBAP) (Project No. NKUBAP.00.M9.AR.14.02

Diyabetik Ratlarda Fesleğen (Ocimum sanctum) Uygulamasının Böbrek Dokusunda Tümör Nekrozis Faktör-α ve İnterlökin 1β Salınımı Üzerine Etkileri

This study aims to examine the changes of the Ocimum sanctum treatment on the tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) in the kidney tissue of the rats, in which the experimental diabetes was induced with streptozotocin (STZ). Forty Sprague Dawley male rats were divided into 5 groups: Diabetes, Diabetes + Ocimum sanctum, Ocimum sanctum, Control, and Sham. The immunohistochemical localization of TNF-alpha and IL-1 beta in the kidney tissue was determined by using the streptavidin-biotin-peroxidase method. Strong TNF-alpha immunoreactivity was determined in the renal cortex of the rats in the Diabetes and Diabetes + Ocimum sanctum groups on 14th days, low immunoreactivity was determined in the rats in Ocimum sanctum, Sham, and Control groups. While strong IL-1 beta immunoreactivity was observed in the renal cortex of the Diabetes group, moderate IL-1 beta immunoreactivity was observed in the renal cortex of the Diabetes + Ocimum sanctum and low immunoreactivity was determined in the Ocimum sanctum, Sham, and Control groups. In this study, it was assessed how the polymorphisms, occurring in the cytokine genes of Ocimum sanctum in the rats, in which experimental diabetes was induced, and TNF-alpha and IL-1 beta, which was demonstrated to have an important role in the complication development in the diabetic patients affected the renal tissue.Ondokuz Mayis UniversityOndokuz Mayis UniversityHistopathological results was evaluation Prof. Dr. Mahmut Sozmen in the patology Depertment Faculty of Veterinary Medicine, Ondokuz Mayis University. Thanks to Mahmut Sozmen for contribution

Malathion ve Onosma nigricaule (Boraginaceae)'nin fare böbrek dokusunda katalaz (CAT) ve süperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkileri]

The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. A total of 48 male mice (Mus musculus) were used in our study. Mice were divided 6 groups (control group, maize oil group, normal saline group, Onosma nigricaule group, malathion group, Onosma nigrcaule+malathion group). Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. The localization of CAT and Mn-SOD in the renal tissue was determined using the method of streptavidinbiotin- peroxidase. CAT immunoreactivity was determined with a weak intensity in epithelium of renal tubulus proximalis of mice in the malathion group, with a moderate intensity in Onosma nigricaule+ malathion group and with a higher intensity in tubulus proximalis of other groups. A cytoplasmic Mn-SOD immunoreactivity was determined with weak intensity in renal medulla of mice in malathion group, with moderate intensity in renal medulla of mice in Onosma nigricaule plant extract+ malathion group, maize oil group, and normal saline group and with highly intensity in control and Onosma nigricaule groups. It was concluded that Onosma nigricaule might play a protective role as an antioxidant against the oxidant features of malathion.Kafkas University, BAPKafkas University [2013-KSYO-78]This research was supported by a grant from the Kafkas University, BAP (Project No: 2013-KSYO-78

Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice

AimThe purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by Malathion that is used as an insecticide in agriculture, on leptin in renal tissues in an immunohistochemical way.Material-MethodsA total of 48 male mice were used in our study. 6 groups were determined as the control (group 1), maize oil which is the transport agent of malathion (group 2), normal saline which is the transport agent of plant extract (group 3), Onosma nigricaule (group 4), malathion (group 5) and Onosma nigricaule plant extract + malathion (group 6) group. The localization of leptin in the renal tissue was determined using the method of Streptavidin-Biotin- Peroxidase. Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations.ResultsNo lesion was determined in renal tissues of the control group, normal saline group, maize oil group, Onosma nigricaule malathion and onosma nigricaule + malathion groups. Leptin immunoreactivity was determined with a weak intensity in renal tubulus proximalis and distalis epithelial cells of mice in the onosma nigricaule group, and onosma nigricaule + malathion group and with a high intensity in tubulus proximalis of other groups.ConclusionThis study conducted on mice revealed that malathion, which is commonly used as insecticide in Turkey, affects leptin activities in renal tissue of mice

Çörek Otu (Nigella Sativa) Uygulanan Sıçanların Pankreas Dokusunda Somatostatin’In İmmünohistokimyasal Dağılımı Ve Gen Ekspresyonu

Bu çalışmada, N. sativa çekirdeğinin özütü olan timokinon (TQ) uygulanmasının ratların pankreas dokusundan salınan somatostatinin immünohistokimyasal lokalizasyonu ve gen ekspresyonu üzerine etkileri incelendi. Çalışmada 30 adet sıçan kontrol (n=10), sham (n=10) ve timokinon (n=10) olmak üzere 3 gruba ayrıldı. Pankreas dokusunun histolojik yapısı Crosman’ın üçlü boyama yöntemiyle incelendi. Pankreas dokusunda somatostatinin ekspresyonu streptavidin-biotin peroksidaz yöntemiyle değerlendirildi. Gen ekspresyonu ise Real-Time PCR ile değerlendirildi. Normal histolojik bulgular saptandı. Tüm grupların endokrin alanlarında (Langerhans adası) spesifik somatostatin immünoreaktivitesi görüldü. Somatostatin salınımının timokinon uygulanan grupta kontrol ve sham gruplarına göre daha fazla olduğu tespit edildi. Gen ekspresyonu ise çoklu denemelere rağmen tespit edilemedi. Sonuç olarak, tedavi edici etkilere sahip olan timokinon uygulanmasının büyüme hormonu, insülin, glukagon, sekretin ve gastrin üzerine baskılayıcı etkileri bilinen somatostatin salınımını arttırdığı tespit edilmiştir.In this study, the effect of Thymoquinone (TQ), which is extracted from the seed of Nigella sativa (N. sativa) supplementation on somatostatin secretion in pancreas tissue of rats were examined by immunohistochemical method and gen expression. In the study, 30 rats were used. Rats were divided into 3 groups as control, sham and thymoquinone. The pancreas tissue was examined by Crossman’s triple staining method. The streptavidin-biotin peroxidase complex method were applied to tissue sections to determine the localization of somatostatin in the pancreas tissue. Normal histological results were obtained. Specific somatostatin immunoreactivities appeared in endocrine parts (islets of Langerhans) of all groups. Somatostatin secretion increased thymoquinone groups compared to control and sham. Somatostatin immunoreactivities in exocrine parts was only appeared in thymoquinone group. But The gene expression was not detected despite multiple attempts. In conclusion, administration of thymoquinone, which has most therepeutic effects, increased somatostatin secretion which is known to inhibit some hormones like as growth hormone, insulin, glucagon, secretin and gastrin

Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice

[Abstract Not Available

Immunohistochemical Examination on the Effects of Malathion and Onosma Nigricaule (Boraginaceae) on the Leptin in Renal Tissues of Mice

Amaç Bu çalışmada, tarımda bir insektisit olarak kullanılan malathion’un neden olduğu oksidasyon parametreleri üzerine Onosma nigricaule (Boraginaceae)’den elde edilen bitkisel özütün böbrek dokusunda leptin üzerine etkisinin immunohistokimyasal olarak araştırılması amaçlandı. Materyal-Metot Çalışmamızda, toplam 48 adet erkek fare kullanıldı. Gruplar; kontrol (grup 1), malathion’un taşıt maddesi olan mısır yağı (grup 2), bitki özütünün taşıt maddesi olan serum fizyolojik (grup 3), malathion (grup 4), Onosma nigricaule (grup 5), ve Onosma nigricaule + malathion grubu (grup 6) olmak üzere toplam 6 grup olarak belirlendi. Böbreklerden alınan kesitlere leptin immunoreaktivitesini belirlemek için Streptavidin-Biotin-Peroksidaz Kompleks tekniği uygulandı. Hazırlanan kesitlere histolojik incelemeler için hematoksilen-eosin ve Crossman’ın üçlü boyama yöntemi kullanıldı. Bulgular Kontrol grubu ile taşıt kontrol grupları olan serum fizyolojik grubu ve mısır yağı grupları ile Onosma nigricaule, malathion ve Onosma nigricaule + malathion gruplarının böbrek dokularında herhangi bir lezyon tespit edilmedi. Diğer yandan, kontrol, mısır yağı, serum ve malathion grubundaki farelerin tubulus proksimalis ve tubulus distalis epitel hücrelerinde güçlü leptin immunoreaktivite görülmesine karşın Onosma nigricaule ve Onosma nigricaule + malathion grubu farelerde ise zayıf leptin immunoreaktivitesi tespit edildi. Sonuç Fareler ile yaptığımız çalışmada, yurdumuzda insektisit olarak yaygın kullanılan malathion’un fare böbreğinde leptin aktivitelerini etkilediği görülmüştür.Aim The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by Malathion that is used as an insecticide in agriculture, on leptin in renal tissues in an immunohistochemical way. Material-Methods A total of 48 male mice were used in our study. 6 groups were determined as the control (group 1), maize oil which is the transport agent of malathion (group 2), normal saline which is the transport agent of plant extract (group 3), Onosma nigricaule (group 4), malathion (group 5) and Onosma nigricaule plant extract + malathion (group 6) group. The localization of leptin in the renal tissue was determined using the method of Streptavidin-BiotinPeroxidase. Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. Results No lesion was determined in renal tissues of the control group, normal saline group, maize oil group, Onosma nigricaule malathion and onosma nigricaule + malathion groups. Leptin immunoreactivity was determined with a weak intensity in renal tubulus proximalis and distalis epithelial cells of mice in the onosma nigricaule group, and onosma nigricaule + malathion group and with a high intensity in tubulus proximalis of other groups. Conclusion This study conducted on mice revealed that malathion, which is commonly used as insecticide in Turkey, affects leptin activities in renal tissue of mice

Capsaicin uygulanan sıçanların duodenumda COX-1, COX-2 ve TGF?-1'in immünohistokimyasal dağılımı]

The purpose of this study was to investigate the effects of capsaicin on the duodenal distribution of cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2) and Transforming growth factor beta-1 (TGF beta-1) in rats. Rats were divided into two groups (n = 10 in each group). Experimental group treated with capsaicin (1 mg/kg, subcutaneously) was injected in 10% ethanol, 1% Tween and 80% distilled water everyday for a period of one week and in not injected control group. Localisations of COX-1, COX-2 and TGF beta-1 were observed in villus and crypt ephitelial cells, and smooth muscle cells. In conclusion, in this study, capsaicin administration was found to increase the release of COX-1 and COX-2, and TGF beta-1

Effects of alpha lipoic acid and vitamin C supplementation on transforming growth factor alpha (TGF alpha) and transforming growth factor beta 2 (TGF beta 2) secretion in testicular tissue of diabetic rats

The effects of alpha lipoic acid and vitamin C supplementation on TGF alpha and TGF beta 2 secretion in the testicular tissue of streptozotocin-induced diabetic rats were examined by immunohistochemical methods. Rats were divided into 5 groups; group 1 (control), 2 (diabetic control), 3 (diabetes + alpha lipoic acid), 4 (diabetes + vitamin C), and 5 (diabetes + alpha lipoic acid + vitamin C). Hematoxylin and eosin staining was performed in order to examine testicular tissue histopathologically. The streptavidin-biotin-peroxidase complex method was used for histological examination and determination of TGF alpha and TGF beta 2 localization in the testicular tissue. Regarding fasting blood glucose levels, the lowest blood glucose level was detected in group 3. Fewer pathological histopathological findings were observed in groups treated with alpha lipoic acid, vitamin C, and alpha lipoic acid + vitamin C compared to the diabetes group. TGF alpha and TGF beta 2 secretions increased in groups 3, 4, and 5 compared to group 2. In conclusion, exogenous administration of alpha lipoic acid, vitamin C, and alpha lipoic acid + vitamin C increased TGF alpha and TGF beta 2 secretion in streptozotocin-induced diabetic rats

Tarçın ekstraktı ıle tedavi edilen diyabetik sıçanların mide dokusunda somatostatinin ımmunohistokimyasal dağılımı]

Diabetes is a chronic metabolic disorder, as well as a situation of increased oxidative stress. We examined the distribution of somatostatin in gastric tissues of cinnamon extract treated streptozotocin-induced diabetic rats using the immunohistochemistry technique. A total of 30 male Sprague Dawley rats were used in the study. The rats were assigned to five groups as control, sham, cinnamon, diabetes and diabetes + cinnamon. No application was made to the control group, the sham group received intraperitoneally (1.p.) 50 mg/kg sodium citrate, and diabetes was induced by i.p. injection of 50 mg/kg STZ in diabetes and diabetes + cinnamon groups. Cinnamon extracts were then given to cinnamon and diabetes + cinnamon groups by oral gavage at a dose of 200 mg/kg for 14 days. The streptavidin-biotin-peroxidase method was used to determine the immunoreactivity of somatostatin. Gastric tissue sections were prepared and stained by Crossman's triple and Hematoxyl in-Eosin staining in order to examine histological structure of the gastric tissue. We determined that somatostatin immunoreactivity of the control, sham and cinnamon groups was stronger than for the diabetes, and diabetes + cinnamon groups. While a weak immunoreactivity was found in the cardia, fundus and pyloric mucosa of the gastric tissue in the diabetes and diabetes + cinnamon groups, a strong immunoreactivity was found in the cinnamon, sham, and control groups. Also, a statistically significant was observed when all groups compared in terms of count of parietal and principal cells (P<0.001). It was determined that there was a statistically significant difference between diabetes, diabetes + cinnamon groups and control, sham, cinnamon groups in terms of fasting blood glucose levels (P<0.05). In conclusion, somatostatin, which plays an important role in gastroduodenal diseases, was found to be lower in the diabetes and cinnamon + diabetes groups