Role of rosuvastatin in bone metabolism of ovariectomized adult rats

The study was planned to explore the influence of one type of hydrophilic statins (rosuvastatin) on the level of serum osteocalcin, N-telopeptide of type 1 collagen (NTx), calcium, and phosphorus. In addition to calcium, phosphorus, and magnesium in femur bone ash of female ovariectomized rats. Thirty female rats aged 2.5-3 months were divided as follows, sham group, ovariectomized (ovx) group as a model of osteoporosis, and (ovx) group treated with 20mg /kg of rosuvastatin for 60 days. Blood samples were collected after 30 and 60 days of the experiment for biochemical analysis. Besides, after 60 days of the treatment, right femur bones were excised and ashed to estimate calcium, phosphorus, and magnesium. The results showed a significant elevation in serum osteocalcin, (NTx), calcium, and phosphorus, in addition to elevation in osteoclasts number and deceased osteoblasts and thickness of trabecular bone in the (ovx) group compared to the sham, while treatment with rosuvastatin caused a significant reduction in osteocalcin, (NTx), calcium and phosphorus after 60 days. Also, the results revealed a significant reduction in the percentage of (Ca, P and Mg) content in bone ash of the (ovx) group compared to the sham group. However, rosuvastatin treatment led to a significant elevation in the percentage of calcium and inorganic phosphate in bone ash and increased the thickness of trabecular bone and development in osteocytes compared with ovariectomized rats. The conclusion of the present study, rosuvastatin has a positive effect on the bone of ovariectomized rats

Effect of atorvastatin on bone formation in ovariectomized rats

The study was designed in order the effect of one class of lipophilic statins (Atorvastatin) in some biomarkers of bone formation (Alkaline phosphatase (ALP), 1,25 (OH)2 D3, calcium, and phosphorous) in serum of ovariectomized female rats. Thirty adult female rats (2.5-3) months, weighing (200-220) gm were housed at conditions of controlled temperature (22-25°C), cycle (12h light - 12h dark) in the house of animals of Veterinary Medicine College of Mosul University. The animals were divided into three equal groups, sham group, ovariectomized (ovx) group, and ovx treatment orally with 20mg/kg/d of atorvastatin. After 60 days of treatment, blood from all groups was collected for ALP activity, 1,25 (OH)2 D3, calcium, and phosphorous estimation, and left femur bones were excised for histological examination. The results showed that the serum ALP, calcium, and phosphorus were significantly elevated, and a significant reduction in 1,25 (OH)2D3 was noticed in the ovx group. However, treatment with atorvastatin caused a significant reduction in ALP with a non-significant elevation in 1,25 (OH)2D3. Histological results showed a low density and thin trabecular bone, a few blood vessels, high numbers of osteoclast, with low numbers of osteoblasts in the ovx group. However, the treated ovx with atorvastatin increases the thickness of trabecular bone, medium developed osteogenic tissue, and a low number of osteoblasts. In conclusion, atorvastatin has a moderate effect on bone of ovx, affecting bone formation more than bone resorption