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Effect of Storage on the Level of Aflatoxin M1 in Milk and Other Dairy Products Sold at Tripoli Province, Libya
Milk and dairy products are one of the chief sources of nutrition for human beings particularly for
infant and children. Aflatoxin M1
(AFM1
) a hydroxylated metabolite of aflatoxin B1 found in milk and
milk productscauses serious health issues for human beings. The objective of this study was to evaluate
the effect of storage on the level of aflatoxin M1
in milk and other dairy products sold at retail stores of
Tripoli Province, Libya. Selected samples (Skimmed and cream milk, infant milk formula, butter, cheese,
Cheddar, spread and slice) were evaluated by using specialized RIDASCREEN AFM1
competitive enzyme
linked immune sorbent assay (ELISA) technique. Our investigation revealed that, the concentration of
AFM1
increased with the duration of storage. Furthermore, we found that the newly manufactured
samples had very low concentration of AFM1
and within the permitted range. Moreover, AFM1
concentration in skimmed and cream milk having 6 month shelf life had 5.00 ngkg-1 and 5.03 ngkg-1
respectively. Furthermore, both the expired skimmed and cream milk had AFM1
concentrations 121.8
ngkg-1 and 108.18 ngkg-1, respectively. In addition to that, we found that the levels of AFM1
in different
dairy products varies with different shelf lives (12 and 1 month), such as cheddar (5.0 and 72.79 ngkg-1),
Spread (5.30 and 60.03 ngkg-1), Slice (5.50 and 61.18 ngkg-1). Additionally, infant milk formula with shelf
life of 24 months and expired samples had AFM1
less than 5.00 ngkg-1 and 60.8 ngkg-1, respectively. Based
on our investigation, we found that the presence AFM1
in milk and milk products at high concentration
may cause serious illness to consumers’ health and the consequent economic losses