SMH-dager i Trondheim med blikk på framtiden

For tredje gang i SMHs historie ble SMH-dagene arrangert i Trondheim, i slutten av oktober. Bak overskriften «Rammer-Roller-Retning» skjulte det seg et variert og bredt sammensatt program av temaer. To fullspekkede dager med fag og framtidsvyer engasjerte og inspirerte de nesten 90 deltakerne som var samlet til semina

A class of DNA-binding peptides from wheat bud causes growth inhibition, G2 cell cycle arrest and apoptosis induction in HeLa cells

<p>Abstract</p> <p>Background</p> <p>Deproteinized DNA from eukaryotic and prokaryotic cells still contains a low-molecular weight peptidic fraction which can be dissociated by alkalinization of the medium. This fraction inhibits RNA transcription and tumor cell growth. Removal from DNA of normal cells causes amplification of DNA template activity. This effect is lower or absent in several cancer cell lines. Likewise, the amount of active peptides in cancer cell DNA extracts is lower than in DNA preparation of the corresponding normal cells. Such evidence, and their ubiquitous presence, suggests that they are a regulatory, conserved factor involved in the control of normal cell growth and gene expression.</p> <p>Results</p> <p>We report that peptides extracted from wheat bud chromatin induce growth inhibition, G₂arrest and caspase-dependent apoptosis in HeLa cells. The growth rate is decreased in cells treated during the S phase only and it is accompanied by DNA damage and DNA synthesis inhibition. In G₂cells, this treatment induces inactivation of the CDK1-cyclin B1 complex and an increase of active chk1 kinase expression.</p> <p>Conclusion</p> <p>The data indicate that the chromatin peptidic pool inhibits HeLa cell growth by causing defective DNA replication which, in turn, arrests cell cycle progression to mitosis via G₂checkpoint pathway activation.</p