Blood glucose, serum proteins levels and superoxide dismutase activity in Cyprinus carpio, Hypophthalmichthys molitrix and Aristichthys nobilis following thermal stress

The study develops a comparative analysis of the mean levels of blood glucose and of total serum proteins, as well as of the activity of superoxide dismutase in one summer-old common carp (Common carp), silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), both prior to and following the wild wintering conditions of the 2006 - 2007 season. Such parameters represent important biochemical markers in stress evaluation, once known that a constantly increasing stress results in higher values of glycemy and in superoxide dismutase activity, although decreasing proteinemy. In the one summer-old fry taken into study, significantly higher post-hibernal values of glycemy - with 84.9% in bighead carp, 139.1% in common carp and 198.1% in silver carp -were recorded. Thermal stress reduces proteinemy with 11% in bighead carp and with 14% in silver carp while, in the case of common carp, spring viremia reduces proteinemy with 13%, comparatively with the mean pre-hibernal values

Correlations between the proteinemy and glycemy of some cyprinidsand the antiparasitary treatments applied

The paper analyzes the modifications produced in some biochemical indices (proteinemy and glycemy), determined in the blood of certain one year-old culture cyprinids, namely: common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), subjected to some prophylactic antiparasitary treatments. The experiment was performed between April 2007 and April 2008, in 0.5 ha ponds, each basin being populated with 79% common carp (245 g/piece), 11% silver carp (475 g/piece) and 10% bighead carp (425 g/piece). In the reference pond, no treatments were applied, while the experimental variant was prophylactically treated both in the moment of ponds filling (April 2007) and during the growing period, with trichlorfon, applied in preventive doses of 0.1 mg/L, in two steps, and calcium chloride, 2 kg/ha, twice a week, respectively. The concentration values of the biochemical indices were determined one year after the experiment (March-April 2008). The results obtained attest that the preventive anti-ectoparasitary treatment applied to the three fish species has positive effects on their physiological condition, generally, on proteinemy and glycemy - especially. In the treated silver carp, spring proteinemy is 23% lower, while glycemy is 35% lower - comparatively with the reference. In the treated common carp, the two biochemical indices show an increasing tendency, with 19% in proteinemy and 27% in glycemy - respectively

Influence of the antiectoparasitary treatment on cellular respiration of some cultured cyprinid species

: The study discusses the influence of a preventive treatment against ectoparasites on the intensity of cellular respiration in three, 3 summer-old cultured cyprinid species, namely: common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis). The cellular consumption of oxygen (μLO2/g fresh tissue) in muscles and gills was determined by the Warburg microrespirometric method, on two batches of fish from each species, namely: a reference (untreated) batch and a batch treated against ectoparasites. The results obtained show that the intensity of the cellular consumption of oxygen differs - as a function of the treatment applied, fish species, cellular type and duration of recordings. Therefore, generally speaking, cellular respiration is more intense in gills than in muscles, in both types of batches: treated and untreated. Lower values are registered in common carp, followed, in increasing order, by those recorded in silver carp and bighead carp. The antiectoparasitary treatment has positive effects, intensifying cellular respiration, along with metabolism stimulation and an improved general physiological condition of fi

Modification of some biochemical parameters and of the concentration of some serum ions in Cyprinus carpio L. species grown under different sanitary-veterinary conditions

The paper discusses the modifications produced in some biochemical indices as well as in the concentration of serum ions, in fry carp, during the period of active feeding, in two experimental ponds of the Iasi district, providing different conditions of ichtyo-pathological prevention. The average values of glucose and alkaline phosphatase from the blood, as well as those of cholesterol, ureic nitrogen, Na+, Ca2+ and K+ from the serum, have been determined after 120 days of experiment (June - September), for 11 representatives of each basin. For the two experimental variants, different preventive treatments - based on calcium chloride administered in three different moments of the growing cycle and on concentrated, drug-containing food for assuring antimicrobial prevention - have been applied. The values recorded in the variant under analysis are lower than those of the reference, with 12.2% for glycemy, 9.7% for total cholesterol, 17.5% for the total ureic nitrogen and 10.98%, respectively, for alkaline phosphatase. The levels of the determined ions show higher values in the experimental variant, with 23.3% for K+ and 4.1%, respectively, for Ca2+, while the average values for Na+ are lower in the fish from variant B, the Na+/K+, Na+/Ca2+ and (Na++K+)/Ca2+ ratios showing inferior values - with 4 - 21% - than the reference ones

SOME PRELIMINARY DATA ON THE TOTAL DNA CONTENT IN HYPOPHTHALMICHTHS MOLITRIX AND ARISTICHTHYS NOBILIS

The work presents a comparative study between the total amount of DNA from five different types of tissues (gills, muscle, liver, spleen, kidneys) in two cultured cyprinids species from the Ezreni accumulation, namely - silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis). The results obtained show that for both Asian cyprinids species the highest average value of the total amount of DNA is to be found in the spleeny tissue comparatively with the muscular tissue, which has the lowest average value, confirming that the spleeny tissue has a mor