7 research outputs found
Π ΠΠΠ¬ Π‘ΠΠΠ ΠΠΠΠΠΠ«Π₯ ΠΠΠΠΠΠΠ¦ΠΠΠΠΠ«Π₯ Π’ΠΠ₯ΠΠΠΠΠΠΠ Π ΠΠΠ ΠΠΠΠΠΠ’ΠΠΠ¬ΠΠΠ ΠΠ ΠΠ¦ΠΠ‘Π‘Π
In recent years, in connection with the reforms of education in our country there is a search for modern effective methods of education and upbringing. The new needs of the developing Russian society cannot be met without a significant restructuring of the methods and technologies of training of highly qualified personnel, implemented in accordance with the requirements of state standards. At the same time, in order to ensure an appropriate level of training of students, and the formation of a specialist with higher education, with all the necessary competencies, it is necessary to introduce new learning technologies in the education process.Given the growing competition in the market of educational services, training of specialists can not limit itself only to traditional lectures, seminars, practical and laboratory work. Of course, the traditional method of teaching remains indispensable in the case of the transfer of theoretical knowledge and concepts. But the formation of deep professional skills and abilities requires other approaches.The introduction of innovative teaching methods will help to improve the quality of training. Modern teaching methods focus on the disclosure of the creative potential of students, increasing their independence in the study of the material.Currently, the implementation of education is largely associated with the use of online learning. Significant information flow on the Internet and the possibilities of distance learning technologies should have a positive impact on the formation of the final skills and abilities. Only a well-organized process of e-learning will lead to the formation of a qualified specialist. This article reveals the relevance and necessity of using modern forms of education in the educational process.Purpose: study of issues related to the possibility of introduction of new technologies and their active use in the educational process.Methodology: theoretical methods (analysis and synthesis, comparison, classification, generalization); methods of empirical level (study of literature, documents and results of activity, evaluation of methods).Results: identification of a number of problems arising from the introduction of active forms of education in the educational process.Practical implications: the results of the study can be taken into account in the organization of the educational process, using active forms of education, both in institutions of higher education and secondary special.Π ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΠ΅ Π³ΠΎΠ΄Ρ Π² ΡΠ²ΡΠ·ΠΈ Ρ ΡΠ΅ΡΠΎΡΠΌΠ°ΠΌΠΈ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π² Π½Π°ΡΠ΅ΠΉ ΡΡΡΠ°Π½Π΅ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ ΠΏΠΎΠΈΡΠΊ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΈ Π²ΠΎΡΠΏΠΈΡΠ°Π½ΠΈΡ. ΠΠΎΠ²ΡΠ΅ ΠΏΠΎΡΡΠ΅Π±Π½ΠΎΡΡΠΈ ΡΠ°Π·Π²ΠΈΠ²Π°ΡΡΠ΅Π³ΠΎΡΡ ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΎΠ±ΡΠ΅ΡΡΠ²Π° Π½Π΅Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΡΡ Π±Π΅Π· ΡΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΠ΅ΡΠ΅ΡΡΡΠΎΠΉΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊ ΠΈ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΊΠ°Π΄ΡΠΎΠ² Π²ΡΡΡΠ΅ΠΉ ΠΊΠ²Π°Π»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ, ΡΠ΅Π°Π»ΠΈΠ·ΡΠ΅ΠΌΡΡ
Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ ΡΡΠ΅Π±ΠΎΠ²Π°Π½ΠΈΡΠΌΠΈ Π³ΠΎΡΡΠ΄Π°ΡΡΡΠ²Π΅Π½Π½ΡΡ
ΡΡΠ°Π½Π΄Π°ΡΡΠΎΠ². ΠΡΠΈ ΡΡΠΎΠΌ Π² ΡΠ΅Π»ΡΡ
ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΡ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΡΡΠΎΠ²Π½Ρ ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΠΎΠ±ΡΡΠ°ΡΡΠΈΡ
ΡΡ, ΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΡΡΠ° Ρ Π²ΡΡΡΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠΌ Π²ΡΠ΅ΠΌΠΈ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΡΠΌΠΈ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠΈΡΠΌΠΈ, ΡΡΠ΅Π±ΡΠ΅ΡΡΡ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ Π½ΠΎΠ²ΡΡ
ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΠΏΡΠΎΡΠ΅ΡΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ.Π£ΡΠΈΡΡΠ²Π°Ρ ΡΠΎΡΡ ΠΊΠΎΠ½ΠΊΡΡΠ΅Π½ΡΠΈΠΈ Π½Π° ΡΡΠ½ΠΊΠ΅ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΡΡ
ΡΡΠ»ΡΠ³, ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠ° ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΡΡΠΎΠ² Π½Π΅ ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ³ΡΠ°Π½ΠΈΡΠΈΠ²Π°ΡΡ ΡΠ΅Π±Ρ Π»ΠΈΡΡ ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½ΡΠΌΠΈ Π»Π΅ΠΊΡΠΈΡΠΌΠΈ, ΡΠ΅ΠΌΠΈΠ½Π°ΡΠ°ΠΌΠΈ, ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΡΠ°Π±ΠΎΡΠ°ΠΌΠΈ. ΠΠ΅Π·ΡΡΠ»ΠΎΠ²Π½ΠΎ, ΡΡΠ°Π΄ΠΈΡΠΈΠΎΠ½Π½Π°Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ° ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ ΠΎΡΡΠ°Π΅ΡΡΡ Π½Π΅Π·Π°ΠΌΠ΅Π½ΠΈΠΌΠΎΠΉ Π² ΡΠ»ΡΡΠ°Π΅ ΠΏΠ΅ΡΠ΅Π΄Π°ΡΠΈ ΡΠ΅ΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π½Π°Π½ΠΈΠΉ ΠΈ ΠΊΠΎΠ½ΡΠ΅ΠΏΡΠΈΠΉ. ΠΠΎ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π³Π»ΡΠ±ΠΎΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
Π½Π°Π²ΡΠΊΠΎΠ² ΠΈ ΡΠΌΠ΅Π½ΠΈΠΉ ΡΡΠ΅Π±ΡΠ΅Ρ ΠΈΠ½ΡΡ
ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠ².ΠΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΊΠ°ΡΠ΅ΡΡΠ²Π° ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΊΠΈ ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΡΡΠΎΠ² ΠΏΠΎΠΌΠΎΠΆΠ΅Ρ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΠ΅ ΠΈΠ½Π½ΠΎΠ²Π°ΡΠΈΠΎΠ½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ. Π‘ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π°ΠΊΡΠ΅Π½ΡΠΈΡΡΡΡΡΠ²ΠΎΠ΅ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ Π½Π° ΡΠ°ΡΠΊΡΡΡΠΈΠΈ ΡΠ²ΠΎΡΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π° ΡΡΡΠ΄Π΅Π½ΡΠΎΠ², ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΈΡ
ΡΠ°ΠΌΠΎΡΡΠΎΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π² ΠΈΠ·ΡΡΠ΅Π½ΠΈΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°.Π Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ ΠΏΡΠΎΡΠ΅ΡΡ ΠΎΡΡΡΠ΅ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π²ΠΎ ΠΌΠ½ΠΎΠ³ΠΎΠΌ ΡΠ²ΡΠ·Π°Π½ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΎΠ½Π»Π°ΠΉΠ½-ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ. ΠΠ½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΏΠΎΡΠΎΠΊ Π² ΡΠ΅ΡΠΈ ΠΠ½ΡΠ΅ΡΠ½Π΅Ρ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ Π΄ΠΈΡΡΠ°Π½ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π΄ΠΎΠ»ΠΆΠ½Ρ Π±Π»Π°Π³ΠΎΠΏΡΠΈΡΡΠ½ΠΎ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡΡΡ Π½Π° ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΡΠΎΠ³ΠΎΠ²ΡΡ
Π½Π°Π²ΡΠΊΠΎΠ² ΠΈ ΡΠΌΠ΅Π½ΠΈΠΉ. Π’ΠΎΠ»ΡΠΊΠΎ Π³ΡΠ°ΠΌΠΎΡΠ½ΠΎ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΠΉ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ»Π΅ΠΊΡΡΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΈΠ²Π΅Π΄Π΅Ρ ΠΊ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠ²Π°Π»ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΡΡΠ°. Π Π΄Π°Π½Π½ΠΎΠΉ ΡΡΠ°ΡΡΠ΅ ΡΠ°ΡΡΠΌΠ°ΡΡΠΈΠ²Π°ΡΡΡΡ Π²ΠΎΠΏΡΠΎΡΡ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΠΈ, Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ ΠΈ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΡΠΎΡΠΌ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠ΅.Π¦Π΅Π»ΡΒ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²ΠΎΠΏΡΠΎΡΠΎΠ², ΡΠ²ΡΠ·Π°Π½Π½ΡΡ
Ρ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΡΡ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΡ Π½ΠΎΠ²ΡΡ
ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΉ ΠΈ ΠΈΡ
Π°ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠ΅.ΠΠ΅ΡΠΎΠ΄ ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ»ΠΎΠ³ΠΈΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ°Π±ΠΎΡΡ:Β ΡΠ΅ΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ (Π°Π½Π°Π»ΠΈΠ· ΠΈ ΡΠΈΠ½ΡΠ΅Π·, ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅, ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ, ΠΎΠ±ΠΎΠ±ΡΠ΅Π½ΠΈΠ΅); ΠΌΠ΅ΡΠΎΠ΄Ρ ΡΠΌΠΏΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ (ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ, Π΄ΠΎΠΊΡΠΌΠ΅Π½ΡΠΎΠ² ΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π΄Π΅ΡΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, ΠΎΡΠ΅Π½ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ).Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ:Β Π²ΡΡΠ²Π»Π΅Π½ΠΈΠ΅ ΡΡΠ΄Π° ΠΏΡΠΎΠ±Π»Π΅ΠΌ, Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡΠΈΡ
ΠΏΡΠΈ Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΡΠΌ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΡΠΉ ΠΏΡΠΎΡΠ΅ΡΡ.ΠΠ±Π»Π°ΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ²:Β ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ ΡΡΡΠ΅Π½Ρ ΠΏΡΠΈ ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°, Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΡΠΌ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ, ΠΊΠ°ΠΊ Π² ΡΡΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡΡ
Π²ΡΡΡΠ΅Π³ΠΎ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ, ΡΠ°ΠΊ ΠΈ ΡΡΠ΅Π΄Π½Π΅-ΡΠΏΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ
Dependence of the Russian Economy on Oil Prices in the Context of Volatility of the Global oil Market: Articulation of Issue
At the present stage, oil is not just commodity but also a kind of asset, the value of which is associated with multiple financial processes. The impact of oil prices is particularly strong in theΒ countries where trade in energy commodities has reached a high level in total exports. Russia belongs to such countries as well. Oil industry is one of the most important areas for supporting the economy development in Russia. Therefore, there is a need to develop measures for proper functioning of the oil industry, as it provides a significant share of Russia's GDP and budget revenues. Besides, about 80% of foreign investment goes directly to the oil and gas sector. In addition, the long-term dependence of theΒ Russian economy on energy resources has led to decline in incentives for the development of other industries and creation of new technologies. The article explores the issues of dependence of the Russian economy on global oil prices and factors that influence the situation on the global oil market; the increasing role of the financial market of oil contracts in the context of the current economic development is substantiated. This article examines the situation when the country's export is largely focused on energy, while other industries significantly lag behind; it is described in the economic theory as aΒ phenomenon dubbed "Dutch disease". This phenomenon is described by increase in extraction and export of commodities; besides, the influx of capital from exports stimulates consumer demand, but the industrial sector fails to keep up with the growth of the household income due to theΒ pressure of the "Dutch disease", which ultimately increases inflation. In addition, this results in lagging of the processing sector of the economy behind the extractive sector.
Keywords: oil market, World Trade Organization, reserve fund, economy
JEL Classifications: O10, F1
The Influence of Processing by Impulse Pressure on the Productivity of the Don Barley (Hordeum vulgare L.)
Plant productivity is the important indicator, which determines the amount of yield. The productivity of plants depends on the number of bruchids per plant and on the weight of 1000 bruchids. The article studies the influence of impulse pressure of various magnitudes on plant productivity of Don barley (Hordeum vulgare L.). It was found that the pressure of 17 MPa was the most effective for increasing the productivity. Impulse pressure of other magnitudes also had influence on the productivity of Don barley
Methods of Seed Germination Stimulation Exemplified by Seeds of Honey Locust (Gleditsia triacanthos)
Seed germination is a difficult process in some cases because these seeds are covered by a firm testa. The firm testa should be destroyed by means of the following agricultural practices: scarification, stratification, impregnation in boiling water, etc. to increase seed germination. The application of different agricultural practices such as pulse pressure 11 MPa and 29 MPa, scarification, stratification, impregnation in boiling water, the treatment by suspension of rhizosphere microorganism V. paradoxus on seed germination of honey locust were discussed in this article. It was found that scarification, stratification and impregnation in boiling water are effective methods of the firm testa seeds treatment. These methods increased the percentage of seed germination significantly. In addition, seeds with natural cracks had high percentage of germination
Managing the Formation of The Individual Financial Culture of University Students
The purpose of the study dealing with the mechanisms for managing the formation of the individual financial culture of university students is to identify the optimal pedagogical conditions that contribute to the development of financially literate behavior of students studying under education agreements as rational consumers of educational services. The work performed allowed concluding that the attitude of students to mastering the chosen educational program, their academic performance, as well as social and labor activity allows predicting the nature of their financial behavior as market actors in their future daily life. The majority of students do not tend to treat their training as an investment in their own human capital due to the predominance of paternalistic attitudes associated with the imposition of costs for education on older family members
The ability of microbial community of Lake Baikal bottom sediments associated with gas discharge to carry out the transformation of organic matter under thermobaric conditions
The ability to compare the composition and metabolic potential of microbial communities inhabiting the subsurface sediment in geographically distinct locations is one of the keys to understanding the evolution and function of the subsurface biosphere. Prospective areas for study of the subsurface biosphere are the sites of hydrocarbon discharges on the bottom of the Lake Baikal rift, where ascending fluxes of gas-saturated fluids and oil from deep layers of bottom sediments seep into near-surface sediment. The samples of surface sediments collected in the area of the Posolskaya Bank methane seep were cultured for 17 months under thermobaric conditions (80Β°Π‘, 5 MPa) with the addition of complementary organic substrate, and a different composition for the gas phase. After incubation, the presence of intact cells of microorganisms, organic matter transformation and the formation of oil biomarkers was confirmed in the samples, with the addition of Baikalian diatom alga Synedra acus detritus, and gas mixture Π‘H4:H2:CO2. Taxonomic assignment of the 16S rRNA sequence data indicates that the predominant sequences in the enrichment were Sphingomonas (55.3%), Solirubrobacter (27.5%) and Arthrobacter (16.6%). At the same time, in heat-killed sediment and in sediment without any additional substrates, which were cultivated in a CH4 atmosphere, no geochemical changes were detected, nor the presence of intact cells and 16S rRNA sequences of Bacteria and Archaea. This data may suggest that the decomposition of organic matter under culturing conditions could be performed by microorganisms from low-temperature sediment layers. One possible explanation of this phenomenon is migration of the representatives of the deep thermophilic community through fault zones in the near surface sediment layers, together with gas-bearing fluids
Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Xylanases (EC 3.2.1.8) hydrolyze the hemicellulose of plant cell walls. Xylanases are used in the food and paper industries and for bioconversion of lignocellulose to biofuel. In this work, the producer-strain with four copies of the xAor xylanase gene was organized in two tandem copies for optimal expression in Komagataella phaffii T07 yeast. The secreted 35 kDa xylanase was purified from culture medium by gel filtration on Sephadex G-25 and anion exchange chromatography on DEAE-Sepharose 6HF. Tryptic peptides of the recombinant enzyme were analyzed by liquid chromatography-tandem mass spectrometry where the amino acid sequence corresponded to Protein Accession # O94163 for Endo-1,4-beta-xylanase from Aspergillus oryzae RIB40. The recombinant xylanase was produced in a bioreactor where the secreted enzyme hydrolyzed oat xylane with an activity of 258240 IU/mL. High activity in the culture medium suggested xylanase could be used for industrial applications without being purified or concentrated. The pH optimum for xylanase xAor was 7.5, though the enzyme was active from pH 2.5 to pH 10. Xylanase was active at temperatures from 35 °C to 85 °C with a maximum at 60 °C. In conclusion, this protocol yields soluble, secreted xylanase suitable for industrial scale production