Эффективность применения n-тирозола для стимулирования регенерации слизистой оболочки губы при экспериментальном стоматите

The purpose of the study is to investigate the effectiveness of the use of n-tyrosol (extract of rhodiola rosea) in the form of gel on the mucous membrane of the lip in rats with experimental stomatitis.Materials and methods. The experiments were carried out on 54 white male rats weighing 220–280 g. The animals were divided into three groups: control, experimental for comparison and experimental main. When modeling chemical stomatitis in rats by applying 5% solution of caustic sodium to the mucous membrane of the lip, the development of inflammation was observed. The local application of the gel based on n-tyrosol was carried out three times a day for 12 days.Histological and macroscopic examination was performed on the 3rd, 7th and 12th days. The extent of damage was recorded by measuring the area of the lesion in square millimeters (mm2 ). At the same time a control material was taken and was fixed in formalin, embedded into paraffin, stained with hematoxylin and eosin by Van Gieson. Specific areas of dense unformed connective tissue, lymphomonocytic infiltration, granulation tissue were determined in the lesion and surrounding tissues; the numerical density of the vessels was determined in 1 mm2 . The data obtained during the study of the dynamics of healing were averaged in the groups mentioned above and subjected to statistical processing using nonparametric (Mann–Whitney and Wilcoxon) methods using the computer program Statistica 8.0.Results. On the 12th day, the area of the lesions was more than twice as large as the surface of burn surfaces, on which the gel with n-tyrosol was used. The use of n-tyrosine-based gel applications reduced by half the specific area of lymphomonocytic infiltration in the surrounding tissues and in the lesion itself, and promoted a more rapid maturation of the connective tissue. Quantitative analysis revealed that the use of the gel with n-tyrosol stimulated the proliferation of granulation and connective tissue, as well as the growth of newly formed vessels in the burn area. Thus, topical application of the n-tyrosine-based gel helped to reduce the inflammatory response and stimulated the regeneration process.Цель исследования. Изучение эффективности применения n-тирозола (экстракт родиолы розовой) в виде геля на слизистую оболочку губы у крыс с экспериментальным стоматитом.Материал и методы. Эксперименты проводились на 54 белых самцах крыс массой 220–280 г. Животные были разделены на три группы: контрольная, экспериментальная сравнения и экспериментальная основная. При моделировании у крыс химического стоматита путем аппликации 5%-м раствором едкого натрия на слизистую оболочку губы наблюдалось развитие воспаления. Местное нанесение геля на основе n-тирозола проводили три раза в день в течение 12 сут.Гистологическое и макроскопическое исследования осуществляли на 3-и, 7-е и 12-е сут. Размеры повреждения регистрировали путем измерения площади очага поражения, мм2. Одновременно производили взятие контрольного материала, который фиксировали в формалине, заливали в парафин, окрашивали гематоксилином и эозином и по Ван Гизону. В очаге поражения и окружающих его тканях определяли удельные площади плотной неоформленной соединительной ткани, лимфомоноцитарной инфильтрации, грануляционной ткани; численную плотность сосудов в 1 мм2 . Данные, полученные в ходе исследования динамики заживления, усредняли по вышеуказанным группам и подвергали статистической обработке с использованием непараметрических (Манна – Уитни и Вилкоксона) методов при помощи компьютерной программы Statistiсa 8.0.Результаты. На 12-е сут площадь участков поражения более чем вдвое превышала площадь ожоговых поверхностей, на которых применяли гель с n-тирозолом. Использование аппликаций геля на основе n-тирозола почти вдвое снижало удельную площадь лимфомоноцитарной инфильтрации в окружающих тканях и в самом очаге поражения, способствовало более быстрому созреванию соединительной ткани. Количественный анализ установил, что применение геля на основе n-тирозола стимулирует пролиферацию грануляционной и соединительной ткани, а также рост новообразованных сосудов в месте ожога. Таким образом, местное нанесение геля на основе n-тирозола способствовало уменьшению воспалительной реакции и стимулировало процесс регенерации

Моделирование функциональных кист яичников путем введения фолликулостимулирующего гормона

Aim. To study the morphofunctional state of ovaries after the introduction of recombinant FSH. Materials and methods. Two groups of mature female Wistar rats were used in the study. The experimental group consisted of 35 rats with a model of follicular ovarian cysts, while 25 rats were in the control group.Rats were taken out of the experiment on days 3, 7, 15, 30 and 60. Paraffin slides of ovaries were stained with hematoxylin and eosin by Van Gieson. Histological and morphometric investigations were performed.Results. On day 7 of FSH administration, the maximum increase in the size of the ovaries was observed due to the formation of single-cell follicular cysts. Rats in the experimental group showed a marked decrease in the number of growing follicles on day 7 and 15. The increase in atretic bodies and follicles in comparison with the control group was observed on day 7 and lasted until the end of the experiment. Additionally, on day 7 of the experiment, hyperemia and vasoconstriction were noted. The number of yellow bodies decreased during the experiment, and an increase in collagen formation occurred starting from day 15 of the experiment.Conclusion. The introduction of follicle-stimulating hormone for 7 days leads to disruption of folliculogenesis, strengthening of atresia in the ovaries, and the formation of functional cysts.Цель исследования. Изучить морфологические изменения яичников при введении рекомбинантного фолликулостимулирующего гормона.Материал и методы. Эксперимент проведен на половозрелых белых самках крыс линии Wistar. Основную группу составили 35 животных с моделью функциональных кист яичников, контрольную – 25 интактных животных. Крысы выводились из эксперимента на 3-и, 7-е, 15-, 30- и 60-е сут. Проведены гистологическое и морфометрическое исследования на депарафинированных срезах яичников, окрашенных гематоксилином и эозином и по Ван Гизону.Результаты. На 7-е сут введения фолликулостимулирующего гормона наблюдалось максимальное увеличение размеров яичников за счет формирования однокамерных функциональных кист. У животных основной группы отмечалось выраженное снижение количества растущих фолликулов на 7- и 15-е сут.Увеличение количества атретических фолликулов и тел по сравнению с таковым в группе контроля наблюдалось с 7-х сут и продолжалось до 60-х сут эксперимента. На 7-е сут эксперимента отмечались гиперемия и полнокровие сосудов. Количество желтых тел уменьшалось в ходе эксперимента. Отмечалось усиление процессов коллагенообразования, начиная с 15-х сут эксперимента.Заключение. Введение фолликулостимулирующего гормона в течение 7 сут приводит к нарушению процессов фолликулогенеза, усилению процессов атрезии в яичниках и формированию функциональных кис

Efficacy of n-tyrozol medication for regeneration stimulation of lip mucous membrane at experimental stomatitis

The purpose of the study is to investigate the effectiveness of the use of n-tyrosol (extract of rhodiola rosea) in the form of gel on the mucous membrane of the lip in rats with experimental stomatitis.Materials and methods. The experiments were carried out on 54 white male rats weighing 220–280 g. The animals were divided into three groups: control, experimental for comparison and experimental main. When modeling chemical stomatitis in rats by applying 5% solution of caustic sodium to the mucous membrane of the lip, the development of inflammation was observed. The local application of the gel based on n-tyrosol was carried out three times a day for 12 days.Histological and macroscopic examination was performed on the 3rd, 7th and 12th days. The extent of damage was recorded by measuring the area of the lesion in square millimeters (mm2 ). At the same time a control material was taken and was fixed in formalin, embedded into paraffin, stained with hematoxylin and eosin by Van Gieson. Specific areas of dense unformed connective tissue, lymphomonocytic infiltration, granulation tissue were determined in the lesion and surrounding tissues; the numerical density of the vessels was determined in 1 mm2 . The data obtained during the study of the dynamics of healing were averaged in the groups mentioned above and subjected to statistical processing using nonparametric (Mann–Whitney and Wilcoxon) methods using the computer program Statistica 8.0.Results. On the 12th day, the area of the lesions was more than twice as large as the surface of burn surfaces, on which the gel with n-tyrosol was used. The use of n-tyrosine-based gel applications reduced by half the specific area of lymphomonocytic infiltration in the surrounding tissues and in the lesion itself, and promoted a more rapid maturation of the connective tissue. Quantitative analysis revealed that the use of the gel with n-tyrosol stimulated the proliferation of granulation and connective tissue, as well as the growth of newly formed vessels in the burn area. Thus, topical application of the n-tyrosine-based gel helped to reduce the inflammatory response and stimulated the regeneration process

Modeling of functional ovarian cysts by introducing a follicle-stimulating hormone

Aim. To study the morphofunctional state of ovaries after the introduction of recombinant FSH. Materials and methods. Two groups of mature female Wistar rats were used in the study. The experimental group consisted of 35 rats with a model of follicular ovarian cysts, while 25 rats were in the control group.Rats were taken out of the experiment on days 3, 7, 15, 30 and 60. Paraffin slides of ovaries were stained with hematoxylin and eosin by Van Gieson. Histological and morphometric investigations were performed.Results. On day 7 of FSH administration, the maximum increase in the size of the ovaries was observed due to the formation of single-cell follicular cysts. Rats in the experimental group showed a marked decrease in the number of growing follicles on day 7 and 15. The increase in atretic bodies and follicles in comparison with the control group was observed on day 7 and lasted until the end of the experiment. Additionally, on day 7 of the experiment, hyperemia and vasoconstriction were noted. The number of yellow bodies decreased during the experiment, and an increase in collagen formation occurred starting from day 15 of the experiment.Conclusion. The introduction of follicle-stimulating hormone for 7 days leads to disruption of folliculogenesis, strengthening of atresia in the ovaries, and the formation of functional cysts

Piezoelectric and Dielectric Electrospun Fluoropolymer Membranes for Oral Mucosa Regeneration: A Comparative Study

Wound healing of the oral mucosa is an urgent problem in modern dental surgical practice. This research article presents and compares the findings of the investigations of the structural, physicochemical, and biological characteristics of two types of polymeric membranes used for the regeneration of oral mucosa. The membranes were prepared from poly(tetrafluoroethylene) (PTFE) and a copolymer of vinylidene fluoride and tetrafluoroethylene (VDF-TeFE) and analyzed via scanning electron microscopy, atomic force microscopy, X-ray diffraction analysis, and Fourier transform infrared spectroscopy. Investigation results obtained indicate that both types of membranes are composed of thin fibers: (0.57 ± 0.25) μm for PTFE membranes and (0.43 ± 0.14) μm for VDF-TeFE membranes. Moreover, the fibers of VDF-TeFE membranes exhibit distinct piezoelectric properties, which are confirmed by piezoresponse force microscopy and X-ray diffraction. Both types of membranes are hydrophobic: (139.7 ± 2.5)° for PTFE membranes and (133.5 ± 2.0)° for VDF-TeFE membranes. In vitro assays verify that both membrane types did not affect the growth and division of mice fibroblasts of the 3T3-L1 cell line, with a cell viability in the range of 88–101%. Finally, in vivo comparative experiments carried out using Wistar rats demonstrate that the piezoelectric VDF-TeFE membranes have a high ability to regenerate oral mucosa