NADPH Oxidases mRNA levels in liver (A) and heart (B) of rats treated with Deca Durabolin (50 mg.mL⁻¹ Organon, 1 mg/100 g bw, im), once a week, for 8 weeks.

<p>mRNA levels were determined by real-time PCR and was expressed relative to the control group. Data were obtained with 10 animals from at least two independent experiments and are shown as mean ± SEM. * p<0.05.</p

Protein carbonyl content of liver (A), kidney (B), and heart (C) of rats treated with Deca Durabolin (50 mg.mL⁻¹ Organon, 1 mg/100 g bw, im), once a week, for 8 weeks.

<p>Protein carbonyl content was evaluated based on a reaction with dinitrophenylhidrazine (DNPH), evaluated in a spectrophotometer at 370 nm. Data were obtained with 6 animals from at least two independent experiments and are shown as mean ± SEM. * p<0.05; **p<0.001.</p

NADPH Oxidase activity in liver (A), kidney (B), and heart (C) of rats treated with Deca Durabolin (50 mg.mL⁻¹ Organon, 1 mg/100 g bw, im), once a week, for 8 weeks.

<p>H₂O₂ production was determined in the microssomal fraction by the Amplex Red/Horseradish Peroxidase assay. Data were obtained with 10 animals from at least two independent experiments and are shown as mean ± SEM. * p<0.05; **p<0.001.</p

Reduced thiol content of liver (A), kidney (B), and heart (C) of rats treated with Deca Durabolin (50 mg.mL⁻¹ Organon, 1 mg/100 g bw, im), once a week, for 8 weeks.

<p>Total sulfhydryl groups were measured by the reaction of thiols with DTNB, evaluated in a spectrophotometer at 412 nm. Data were obtained with 6 animals from at least two independent experiments and are shown as mean ± SEM. * p<0.05; **p<0.001.</p