From the Wild to the Field: Documentation, Propagation, Pilot Cultivation, Fertilization, and Phytochemical Evaluation of the Neglected and Underutilized Amelanchier ovalis Medik. (Rosaceae)

The snowy Mespilus, or serviceberry (Amelanchier ovalis Medik., Rosaceae) represents a neglected and underutilized small fruit tree species with high nutritional value. In this work, we present the results of a long-term study facilitating the sustainable exploitation of A. ovalis as a new germplasm resource from the Greek flora. Ten wild-growing population samples of A. ovalis have been collected from natural habitats in northern Greece. Asexual propagation trials on these materials delivered successful propagation (83.3% rooting) on a selected genotype via leafy cuttings of young, primary, non-lignified soft wood with the application of the rooting hormone. The ex situ cultivation potential of the selected genotype has been evaluated under distinct fertilization regimes in a pilot field trial. Three-year results of this ongoing trial have shown that A. ovalis does not require external nutrient enhancement to be established during its early stages since plant growth rates between conventional fertilization and control plants were similar for the first two years and higher compared to organic fertilization. Conventional fertilization delivered higher fresh fruit production in the third year, with higher fruit number and fruit size compared to organic fertilization and control plants. The phytochemical potential of the cultivated genotype was assessed via the total phenolic content and radical scavenging activity of separate extracts from leaves, twigs, flowers, and young fruits, which revealed that individual plant organs have strong antioxidant activity despite their moderate total phenolic content. The multifaceted approach applied herein has provided novel data that may set the framework for further applied research toward the sustainable agronomic exploitation of Greek A. ovalis as a diversified superfood crop

Documenting Greek Indigenous Germplasm of Cornelian Cherry (Cornus mas L.) for Sustainable Utilization: Molecular Authentication, Asexual Propagation, and Phytochemical Evaluation

Wild-growing Cornelian cherries (Cornus mas L., Cornaceae) are well-known native fruits in Greece since ancient times that are still consumed locally nowadays. Modern research has highlighted the value of Cornelian cherries as functional food with exceptional health benefits on account of the fruits’ biochemical profile. However, apart from local consumption directly from wild growing individuals, Greek native C. mas populations have not yet been investigated or sustainably utilized. A multifaceted evaluation was conducted herein including authorized collection-documentation, taxonomic identification, and molecular authentication (DNA barcoding), asexual propagation via cuttings and phytochemical evaluation (multiple antioxidant profiling) of neglected and underutilized Greek native C. mas germplasm sources. Successive botanical expeditions resulted in the collection of 18 samples of genotypes from distant C. mas populations across different natural habitats in Greece, most of which were DNA fingerprinted for the first time. Asexual propagation trials revealed high variability in rooting frequencies among Greek genotypes with low (<25%), average (25–50%), and adequate propagation potential (>50%) using external indole-3-butyric acid (IBA) hormone application on soft- or hard-wood cuttings. The comparative phytochemical evaluation of the studied Greek genotypes showed significant potential in terms of antioxidant activity (>80% radical scavenging activity in 13 genotypes), but with variable phenolic content (47.58–355.46 mg GAE/100 g), flavonoid content (0.15–0.86 mg CE/100 g), and vitamin C content (1–59 mg AAE/100 g). The collected material is currently maintained under ex situ conservation for long-term monitoring coupled with ongoing pilot cultivation trials. The pivotal data create for the first time a framework for the sustainable utilization of Greek native C. mas germplasm as a superfood with significant agronomic potential

Molecular Authentication, Propagation Trials and Field Establishment of Greek Native Genotypes of Sambucus nigra L. (Caprifoliaceae): Setting the Basis for Domestication and Sustainable Utilization

In the context of sustainable utilisation of valuable but neglected plant resources, a multifaceted study is presented herein for Greek native genotypes of elderberry (Sambucus nigra L., Caprifoliaceae), a species with an appreciated pharmaceutical and nutritional value. At the first phase, fresh plant materials (leaves, softwood cuttings) were collected from nine geographically separated genotypes originating from wild-growing Greek native germplasm of S. nigra. The leaf samples were genetically authenticated using DNA barcoding (ITS2). The next phase included the development of an asexual propagation protocol via cuttings which included screening of the collected genotypes in terms of propagation success, and further experimentation over a two-year period on a prioritised genotype. The propagation results highlighted the importance of external application of indole-3-butyric acid (IBA) rooting hormone at levels of 2000–4000 ppm, which consistently presented high rooting rates (100%) of summer softwood, leafy cuttings of apical or sub-apical type. At the same time, rooting quality in terms of root number and length as well as early plant growth after rooting, were improved by external hormone application resulting in high rates of plant survival. This study reports first-time data on multifaceted assessment of Greek native S. nigra genotypes on molecular authentication and asexual propagation, thus ultimately setting the basis for domestication and sustainable utilization of this species

Investigation of plant-environment interactions affecting high temperature induced reductions in cropping in everbearing strawberries (Frageria x ananassa Duch.)

The work in this project aimed to shed light on the basis of high temperature induced reductions in cropping in everbearing strawberries. This would enhance the understanding of the physiology of this response in everbearing strawberries, and contribute to the development of practical methods to alleviate its severity on a commercial scale. Two physiological components have been revealed by the research: first, the reduction in flower production following a high temperature episode. Secondly, post-anthesis flower abortion and the potential importance of pollen performance. In flowers which were emerging under high temperatures, pollen showed reduced germination capacity. This is suggested to be a contributory factor to the observed post-anthesis flower abortion, which resulted in significant proportions of flowers not setting fruit. As a result, the final crop did not reflect the number of flowers produced by the plant and there was a high temperature-induced cropping trough. As far as practical methods are concerned, the interaction of other environmental factors with temperature was not found to alleviate the adverse effects of high temperature on cropping. The factors studied were vapour pressure deficit in the growth environment, and osmotic potential of the substrate (electrical conductivity of the feed solution). Crop load reduction (Le. truss removal) was also studied as a potential means of alleviating high-temperature induced cropping troughs, but the cropping patterns of treated plants did not differ significantly from those of the non- treated plants. Finally, poly tunnel cooling technologies were evaluated and it was found that forced air ventilation incorporated into a tunnel with a light diffusive cladding material with a degree of internal shading up to 35% could provide temperature control. This therefore could provide the potential to reduce temperature inside polytunnels and so ameliorate cropping troughs following periods of high summer temperatures.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

GIS-Facilitated Germination of Stored Seeds from Four Wild-Growing Populations of <i>Petromarula pinnata</i> (L.) A. DC.—A Valuable, yet Vulnerable Local Endemic Plant of Crete (Greece)

The ex situ conservation and sustainable exploitation of neglected or underutilized plant species (NUPs) is an urgent and vital endeavor. To this end, we focused on Petromarula pinnata (Campanulaceae), a vulnerable local plant endemic to Crete (Greece) that has been garnering interest for its agro-alimentary, medicinal, and ornamental value. A GIS ecological profile was established herein based on the natural distribution of this species in Crete. This profile contains detailed information on the climatic conditions (minimum, maximum, and mean temperatures; precipitation), as well as information on 19 bioclimatic variables that shape its natural adaptations. This profiling contributed to a better understanding of the species’ ecological requirements and facilitated germination trials employing stored seeds from four distinct populations (two from lowlands and two from semi-mountainous areas) at four temperatures (10, 15, 20, and 25 °C) and two light conditions. The results presented here show that both incubation temperature and population of origin, as well as the interaction between these variables, significantly affected seed germination rates. Incubation temperatures of 10 and 15 °C were the most appropriate for the successful germination of this species (>81.25% for both temperatures in three out of four populations), with light conditions having no effect on seed germination (86% in light and 80% in darkness). The establishment of a protocol for the successful germination of P. pinnata seeds opens avenues for further sustainable exploitation of this valuable yet vulnerable NUP as a new Greek native crop

Vase Life Evaluation of Three Greek Tulip Species Compared with a Commercial Cultivar

Aiming at evaluating new candidate species for the cut flower market of Greece and beyond, in this study, the vase life of three Greek tulip plant species, Tulipa cretica ‘Hilde’ (CRH, local endemic of Crete, Greece), T. clusiana ‘Chrysantha’ (CLC, naturalized in Chios Island, Greece), and T. australis (AUS, native in the Mediterranean and Greece), was investigated in comparison to the commercial tulip hybrid Île-de-France (IDF). To this end, pre-cooled at 4 °C bulbs of the abovementioned Greek tulip plant species were bought from Dutch nurseries and grown in pots placed in unheated greenhouses located at two different climatic conditions in Northern Greece. The plants were uprooted when the flowers reached a slightly open stage. Half of the flowering stems were immediately placed into bottles with deionized water, while the rest were placed in a preservative solution containing citric acid 5% and sulfuric acid 1% and then remained under laboratory conditions until the entire tepal wilted (end of vase life). The measurements performed concerned: (a) flower stem length and flower maximum diameter, (b) fresh weight (FW) of initial stems, leaves, flowers, and bulbs and at the end of vase life, (c) flower color parameters (L, a, b, c, and H) in all treated flowers, (d) leaf chlorophyll content (SPAD values), and (e) initial and final water volume after removing the flowering stems. The aforementioned measurements showed that CRH cut flowers may exhibit consistent floral opening patterns and were associated with a long mean vase life of 5.7 days, which can be further prolonged to 6.5 days by carefully selecting a cultivation location with proper climatic conditions. The vase life of CLC cut flowers was significantly affected by the climatic parameters (temperature) of the area where the plants were cultivated. The immersion of cut flowering stems in a preservative solution with citric and sulfuric acids did not yield a notable increase in the longevity of cut flowers during the postharvest period. Moreover, this treatment did not have any significant impact on leaf chlorophyll content or flower color at the end of the flowers’ vase life. The data of this work show that cut flowers from the native species T. cretica and T. clusiana have satisfactory vase life, especially when plants were grown in favorable climate condition; the latter is an important criterion for their entry into the cut flower market

GIS-Facilitated Germination of Stored Seeds from Five Wild-Growing Populations of <i>Campanula pelviformis</i> Lam. and Fertilization Effects on Growth, Nutrients, Phenol Content and Antioxidant Potential

This study was designed to bridge extant research gaps regarding the vulnerable and protected local endemic Campanula pelviformis, a wild edible green traditionally consumed in Crete (Greece) with agro-alimentary and medicinal interest as well as ornamental value. The C. pelviformis ecological profile was generated using the climate and temperature conditions prevailing in its wild habitats through mapping of natural distribution linked with online bioclimatic databases in geographical information systems. We tested the germination of seeds from five wild-growing populations at four different temperatures (10, 15, 20 and 25 °C) and under different light conditions (light/dark and darkness), and we performed fertilization trails [integrated nutrient management (INF), chemical fertilization (ChFe), control] examining morphological and physiological characteristics, above- and below-ground macro- and micronutrients and phenol contents, as well as their antioxidant capacity. We found population and temperature effects on seed germination with their interaction being statistically significant. Campanula pelviformis germinated better at 10 and 15 °C (>85% for all populations) with no preference on light conditions (98.75% and 95% in light and dark conditions). The INF application increased root dry mass, chlorophyll content index and chlorophyll fluorescence compared to other treatments and was beneficial for macro- and micronutrient concentrations in above-ground parts compared to previously studied wild-growing material, while below-ground parts were positively impacted by both fertilization types. Total phenols and antioxidant capacity were both increased by ChFe fertilization. The data furnished herein permitted the re-evaluation and upgrade of its sustainable exploitation potential in different economic sectors

Assessing the Economic Performance of Multipurpose Collaborative Robots toward Skillful and Sustainable Viticultural Practices

The increased cost of labor in modern viticulture stemming from the nature of operations that require physical strength and precision, coupled with labor shortages, poses a significant constraint in facilitating and scheduling seasonal activities. Therefore, autonomous collaborative robots present a potential solution for achieving sustainable development objectives and decreasing operational expenditures in agricultural operations. The current paper presents an economic assessment of collaborative robots (or cobots for short) in comparison to conventional labor for four different cultivars in Greece in a lifecycle costing methodological framework. The selected cultivars are Asyrtiko, Cabernet Sauvignon, Merlot and Tempranillo, which are cultivated by two private wineries in the area of interest. All the relevant expenses of their annual production were distributed to agricultural operations, and eight scenarios were developed to compare conventional and cobot practices. The results indicate the great potential of cobots regarding specific viticultural operations such as weed control, pruning, herbiciding and topping. The adoption of cobots in these operations has the potential to contribute to sustainable agriculture by reducing labor costs and addressing labor shortages, while also increasing the efficiency and precision of these tasks. Nevertheless, the defoliation and tying operations appeared to be inefficient in most cases in comparison to conventional labor practices. Overall, the annual equivalent costs could be reduced by up to 11.53% using cobots, even though the projected lifetime of the cobots plays a significant role in the cost-effectiveness of autonomous robotic labor in viticulture. In conclusion, cobots could be instrumental in the Greek viticulture, integrating innovation and high-quality products toward sustainable agricultural development

Documenting Greek Indigenous Germplasm of Cornelian Cherry (<i>Cornus mas</i> L.) for Sustainable Utilization: Molecular Authentication, Asexual Propagation, and Phytochemical Evaluation

Wild-growing Cornelian cherries (Cornus mas L., Cornaceae) are well-known native fruits in Greece since ancient times that are still consumed locally nowadays. Modern research has highlighted the value of Cornelian cherries as functional food with exceptional health benefits on account of the fruits’ biochemical profile. However, apart from local consumption directly from wild growing individuals, Greek native C. mas populations have not yet been investigated or sustainably utilized. A multifaceted evaluation was conducted herein including authorized collection-documentation, taxonomic identification, and molecular authentication (DNA barcoding), asexual propagation via cuttings and phytochemical evaluation (multiple antioxidant profiling) of neglected and underutilized Greek native C. mas germplasm sources. Successive botanical expeditions resulted in the collection of 18 samples of genotypes from distant C. mas populations across different natural habitats in Greece, most of which were DNA fingerprinted for the first time. Asexual propagation trials revealed high variability in rooting frequencies among Greek genotypes with low (50%) using external indole-3-butyric acid (IBA) hormone application on soft- or hard-wood cuttings. The comparative phytochemical evaluation of the studied Greek genotypes showed significant potential in terms of antioxidant activity (>80% radical scavenging activity in 13 genotypes), but with variable phenolic content (47.58–355.46 mg GAE/100 g), flavonoid content (0.15–0.86 mg CE/100 g), and vitamin C content (1–59 mg AAE/100 g). The collected material is currently maintained under ex situ conservation for long-term monitoring coupled with ongoing pilot cultivation trials. The pivotal data create for the first time a framework for the sustainable utilization of Greek native C. mas germplasm as a superfood with significant agronomic potential

Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation

Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing Rosa canina populations of Greece were selected for investigation and evaluation of their potential for integrated domestication. We collected ripe rosehips from Greek native wild-growing populations (samples from seven genotypes) for phytochemical analysis (total phenolics, total flavonoids, antioxidant activity and vitamin C content), leaf samples for DNA analysis using the ITS2 sequence (nine genotypes) and fresh soft-wood stem cuttings for propagation trials (seven genotypes). After evaluation of these materials, this study reports for the first-time distinct DNA-fingerprinted genotypes from Greece with interesting phytochemical profiles mainly in terms of Vitamic C content (up to 500.22 &plusmn; 0.15 mg of ascorbic acid equivalents/100 g of sample) as well as effective asexual propagation protocols for prioritized R. canina genotypes via cuttings. The latter highlights the importance of the levels of external hormone application (2000 ppm of indole-3-butyric acid), the effect of season (highly-effective spring trials) and genotype-specific differences in rooting capacities of the studied genotypes. All inclusive, this study offers new artificially selected material of Greek native R. canina with a consolidated identity and interesting phytochemical profile. These materials are currently under ex-situ conservation for further evaluation and characterization in pilot field studies, thus facilitating its sustainable exploitation for applications in the agro-alimentary, medicinal-cosmetic, and ornamental sectors