A NEW SPECIES OF THE SPIDER GENUS ANYPHAENOIDES FROM BRAZILIAN CAATINGA (ARANEAE, ANYPHAENIDAE, ANYPHAENINAE)

Volume: 31Start Page: 145End Page: 14

Two new tiny Nemesiidae species from Reserva Biológica do Tinguá, Rio de Janeiro, Brazil (Araneae: Mygalomorphae)

Two new Nemesiidae species from Reserva Biológica do Tinguá, Rio de Janeiro, Brazil are described. Acanthogonatus minimussp. nov. differs from the remaining species of the genus by the male palpal bulb, which has very long and twisted embolus, ca. 2/3 the length of the palpal tibia, long and twisted spermathecae, anterior eye row recurved and fovea T-shaped. Chaco tinguasp. nov. differs from the remaining species of the genus by the retrolateral megaspine on tibia I, palpal embolus tip hook-shaped, inferior tarsal claw on all legs, absence of pubescence on the carapace and legs. Both species were collected with Winkler extractors in leaf litter. They are the smallest specimens in their respective genera and also among the world Nemesiidae described to date. The smallest male of A. minimussp. nov. measures 4.22 mm and of C. tinguasp. nov. measures 3.85 mm. Data on the phenology of C. tinguasp. nov. is presented

Triatoma rubrovaria (Blanchard, 1843) (Hemiptera - Reduviidae - Triatominae) IV: bionomic aspects on the vector capacity of nymphs

Triatoma rubrovaria has become the most frequently captured triatomine species since the control of T. infestans in the state of Rio Grande do Sul (RS), Brazil. The aim of this study was to evaluate aspects of the vectorial competence of T. rubrovaria using nymphs raised in laboratory under environmental conditions of temperature and humidity and fed on mice. The average developmental period of T. rubrovaria was 180.1 days. The percentage of defecation shortly after feeding was still higher than previous studies in which samples of T. rubrovaria subjected to a slight starvation period before the blood meal were used. The obtained results support former indication that T. rubrovaria presents bionomic characteristics propitious to be a good vector of Trypanosoma cruzi to man. Therefore its domiciliary invasion process must be continuously monitored

Vector capacity of members of Triatoma brasiliensis species complex: The need to extend Chagas disease surveillance to Triatoma melanica

Submitted by sandra infurna ([email protected]) on 2016-07-03T20:11:29Z No. of bitstreams: 1 carlos_almeida_etal_IOC_2016.pdf: 310645 bytes, checksum: ba89a5967da32fba1631a09db570cdab (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-03T20:29:21Z (GMT) No. of bitstreams: 1 carlos_almeida_etal_IOC_2016.pdf: 310645 bytes, checksum: ba89a5967da32fba1631a09db570cdab (MD5)Made available in DSpace on 2016-07-03T20:29:21Z (GMT). No. of bitstreams: 1 carlos_almeida_etal_IOC_2016.pdf: 310645 bytes, checksum: ba89a5967da32fba1631a09db570cdab (MD5) Previous issue date: 2016Submitted by Angelo Silva ([email protected]) on 2016-07-07T11:16:57Z No. of bitstreams: 3 carlos_almeida_etal_IOC_2016.pdf.txt: 33353 bytes, checksum: fb06d78000a9971855fb491ec56e0c7b (MD5) carlos_almeida_etal_IOC_2016.pdf: 310645 bytes, checksum: ba89a5967da32fba1631a09db570cdab (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-07T12:23:18Z (GMT) No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos_almeida_etal_IOC_2016.pdf: 310645 bytes, checksum: ba89a5967da32fba1631a09db570cdab (MD5) carlos_almeida_etal_IOC_2016.pdf.txt: 33353 bytes, checksum: fb06d78000a9971855fb491ec56e0c7b (MD5)Made available in DSpace on 2016-07-07T12:23:18Z (GMT). No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos_almeida_etal_IOC_2016.pdf: 310645 bytes, checksum: ba89a5967da32fba1631a09db570cdab (MD5) carlos_almeida_etal_IOC_2016.pdf.txt: 33353 bytes, checksum: fb06d78000a9971855fb491ec56e0c7b (MD5) Previous issue date: 2016Universidade Federal da Paraíba-UFPB. Programa de Pós-Graduação em Ecologia e Monitoramento Ambiental - PPGEMA. Laboratório Ecologia Animal. João Pessoa, PB, BrasilUniversity of Wisconsin-Platteville.Department of Geography and Geology. WI, USA.Centro Federal de Educação Tecnológica Celso Suckow da Fonseca - CEFET/RJ. Campus Valença. Valença, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.Universidade Federal da Paraíba-UFPB. Programa de Pós-Graduação em Ecologia e Monitoramento Ambiental - PPGEMA. Laboratório Ecologia Animal. João Pessoa, PB, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biodiversidade Entomológica. Rio de Janeiro, RJ, Brasil.Universidade Federal da Paraíba-UFPB. Programa de Pós-Graduação em Ecologia e Monitoramento Ambiental - PPGEMA. João Pessoa, PB, Brasil.We conducted a lab-based comparative study on vector capacity features of two species of triatomines: Triatoma brasiliensis and T. melanica. Both are members of the T. brasiliensis species complex. The former is the most important Chagas disease vector in the northeastern region of Brazil. To date, no transmission via T. melanica has been recorded. Immature insects exhibited distinct intermoult periods without a direct relationship to a given species. Females of T. brasiliensis consumed an average of 1.9 times more meals (mean = 12.92 vs 6.63) and survived for a shorter period (mean =330.8 days) than T. melanica (mean = 365.2 days), probably due to the cost of reproduction (all significant at P39%) of insects defecated rapidly (<30 s) after feeding. Overall, results highlight the need to extend vector surveillance to T. melanica

Phylogenomics for Chagas Disease Vectors of the Rhodnius Genus (Hemiptera, Triatominae): What We Learn From Mito-Nuclear Conflicts and Recommendations

International audienceWe provide in this study a very large DNA dataset on Rhodnius species including 36 samples representing 16 valid species of the three Rhodnius groups, pictipes, prolixus and pallescens. Samples were sequenced at low-depth with whole-genome shotgun sequencing (Illumina technology). Using phylogenomics including 15 mitochondrial genes (13.3 kb), partial nuclear rDNA (5.2 kb) and 51 nuclear protein-coding genes (36.3 kb), we resolve sticking points in the Rhodnius phylogeny. At the species level, we confirmed the species-specific status of R. montenegrensis and R. marabaensis and we agree with the synonymy of R. taquarussuensis with R. neglectus. We also invite to revisit the species-specific status of R. milesi that is more likely R. nasutus . We proposed to define a robustus species complex that comprises the four close relative species: R. marabaensis, R. montenegrensis, R. prolixus and R. robustus. As Psammolestes tertius was included in the Rhodnius clade, we strongly recommend reclassifying this species as R. tertius . At the Rhodnius group level, molecular data consistently supports the clustering of the pictipes and pallescens groups, more related to each other than they are to the prolixus group. Moreover, comparing mitochondrial and nuclear tree topologies, our results demonstrated that various introgression events occurred in all the three Rhodnius groups, in laboratory strains but also in wild specimens. We demonstrated that introgressions occurred frequently in the prolixus group, involving the related species of the robustus complex but also the pairwise R. nasutus and R. neglectus . A genome wide analysis highlighted an introgression event in the pictipes group between R. stali and R. brethesi and suggested a complex gene flow between the three species of the pallescens group, R. colombiensis, R. pallescens and R. ecuadoriensis . The molecular data supports also a sylvatic distribution of R. prolixus in Brazil (Pará state) and the monophyly of R. robustus . As we detected extensive introgression events and selective pressure on mitochondrial genes, we strongly recommend performing separate mitochondrial and nuclear phylogenies and to take advantages of mito-nuclear conflicts in order to have a comprehensive evolutionary vision of this genus