The sphenopalatine foramen in man: anatomical, radiological and endoscopic study

Background: Epistaxis is a frequent problem otorhinolaryngologists faces of in their practice. The variations of the sphenopalatine foramen (SPF) and consequ­ently the artery may be one of the major sources of such bleeding. The present work aimed to localise the site of SPF and also to illustrate its different shapes, number and any other variation. Materials and methods: In the current study, 20 adult skulls of both sexes with total 40 half skulls were used in addition to 20 heads of adult living subjects of both sexes aged between 30 and 60 years examined with multislice helical com­puted tomography with 3-dimensional reconstruction of SPF. Then, examination of another ten dried skulls with endoscope was performed. Results: The number of the SPF is varied being single in nearly 80% and multiple in 20% of examined cases. The shape of the foramen also is varied; regular in 67.5% and irregular in 32.5% of all cases. The site of the foramen on the lateral nasal wall is placed in the superior meatus in most of examined skulls (62.5%) while in the rest (37.5%) they are found in the superior meatus and extending to the middle one. Conclusions: There are variation of the number, shape and site of the SPF, and consequently of the branches of the sphenopalatine artery, and this may explain the surgical failure in management of severe epistaxis. The data obtained from the current work support the predication of more than one sphenopalatine ar­teries and gives ample knowledge on the endoscopic study of the lateral nasal wall and consequently the surgical treatment of severe epistaxis. (Folia Morphol 2018; 77, 2: 345–355

Aging changes in the retina of male albino rat: a histological, ultrastructural and immunohistochemical study

Background: Degenerative changes caused by aging may affect the eye, especially the retina. Such changes occur as a part of normal physiological process and may be irreversible. The aim of the study was to demonstrate the influence of aging on the morphology of the retina to provide a basis to explain the pathogenesis of age-associated decline in visual acuity, scotopic and photopic sensitivity.  Materials and methods: Forty male albino rats were used and divided into four age groups (group I: age of cortical maturity, group II: middle-aged, group III: aged group and group IV: senile group). The rats were sacrificed, the eye balls were enucleated. Intra-vitreal injections of formalin for haematoxylin and eosin and immunohistochemical sections, glutaraldehyde for toluidine blue semithin and E/M ultra-thin sections were performed. Measurements and quantitative histomorphometric estimation of the layers of the retina were done.  Results: Light microscopic examination revealed age-dependent attenuation of photoreceptor striations. Aged and senile groups presented pyknotic, widely- -spaced nuclei of the outer nuclear layer. The inner nuclear layer was thinned out to 2 or 3 cellular rows. Retinal capillaries showed progressive dilatation and congestion. Statistical analysis proved significant thinning of the retina with variable degrees of thinning of the constituting layers. Decreased arborisation with age was confirmed with quantification of synaptophysin-immunostained sections. Glial fibrillary acidic protein immunostaining revealed the picture of reactive gliosis. On the ultrastructural level, the retinal pigment epithelium exhibited major alterations with aging. Numerous phagosomes, lipofuscin and melanolipofusin granules appeared within the cells, together with exaggerated basal infoldings. The pho- toreceptor nuclei became degenerated and the perinuclear space was widened. Conclusions: Rat retinae clearly undergo age-related morphological changes. Such changes provide a cellular base for explanation of decreased vision in humans with aging other than reflection errors. Effect of aging was not only qualitative, but also quantitative

Can constant light exposure affect the thyroid gland in prepubertal male albino rats? Histological and ultrastructural study

Background: Through scientific literature, there is evidence that light affects thyroid function in human, mice and rabbits. Constant light and sleep deprivation is also used as a form of human torture, as it has impact on cognitive performances. The present work was conducted to study the effect of constant light for short and long periods on the thyroid gland in the prepubertal male albino rats.  Materials and methods: A total of 30 prepubertal male albino rats were used. The rats separated into three groups: group I (control); group II were those rats put under steady encompassing light (24 h/day, light intensity of 600 lux) for 4 weeks; and group III were the rats maintained in constant light for 3 months. The rat thyroid gland was subjected to histological and ultrastructural examination.  Results: The rats exposed to light for long durations showed disturbed architecture; the follicles exhibited back to back arrangement (signs of hypertrophy with hyperplasia), lined by multiple layers of follicular cells or were lined by vacuolated cells. Few thyroid follicles exhibited cystic hyperplasia. Congested blood capillaries were demonstrated between the follicles.  Conclusions: It can be concluded that the short-term exposure to constant light for 1 month had no apparent effect on thyroid gland tissues while longer exposure to light for 3 months had detrimental effects on the thyroid gland structure of male albino rats.