5 research outputs found
Bactericidal activity of manganese (IV) complex of 2-methylamino-pyridine against Streptococcus pyogenes and Staphylococcus aureus
Objective: To investigate the bactericidal activity of manganese (IV)
complex of 2-methylamino-pyridine against Streptococcus pyogenes
( S. pyogenes ) and Staphylococcus aureus ( S. aureus ).
Materials and Methods: The inhibitory effect of the complex was
studied on the molecular level and by turbidity measurement. Treatment
of bacteria was carried out using 5, 10, 25, 50 and 100 µmol of
the complex per ml of culture media. Results: The results showed that
the growth of S. pyogenes rapidly decreased with increasing
concentrations of the complex. In contrast, the complex caused no
significant decrease in the growth rate of S. aureus . The molecular
level studies showed that four protein bands, with apparent molecular
weights of 19, 23, 30 and 54 Kda, respectively, increased in the
protein pattern of the S. pyogenes extract after the complex treatment
using silver stained polyacrylamide gels, under reducing condition .
However, there was no detectable change in the protein pattern of the
S. aureus extract after the complex treatment . No DNA damage was
detected while using agarose gel electrophoresis and ethidium bromide
staining in both types of bacteria. Conclusion: Manganese (IV)
complex of 2-methylamino-pyridine showed an apparent antibacterial
inhibitory effect against S. pyogenes, but S. aureus was apparently
resistant