Primer Informe de Bacterias Endofíticas Aisladas de Senecio glaucus L., Egipto

Microorganisms are naturally associated with plants in several ways. The study was conducted to isolate bacteria endophytes from the internal cells of roots, stems, leaves, and capitula of Senecio glaucus collected from 2 diverse (coastal and desert) habitats in Egypt. A total of 10 endophytic bacteria were obtained from the isolation; the highest diversity of bacterial endophytes was observed in desert samples roots and leaves. The isolates were recognized based on morphology, biochemical and 16S rRNA sequence genes. All isolates indicated the ability for enzyme production as amylase, cellulase, lipase, catalase, and protease in their biochemical descriptions; analyses also gave a significant indication of their potential to produce plant growth hormones, as their ability to dissolve Phosphate. In the world and Egypt, we are the first to report bacterial endophytes isolated from Senecio glaucus. This study could aid in determining the role of endophytic bacteria in severe habitats, as well as their potential applications in medicine, bioremediation, agriculture, and industry.Los microorganismos están naturalmente asociados con las plantas. El presente experimento se llevó a cabo para aislar bacterias endófitas de las células internas de raíces, tallos, hojas y Tejido capitulear de Senecio glaucus recolectadas en 2 hábitats diversos (costeros y desérticos) de Egipto. Del aislamiento se obtuvieron un total de 10 bacterias endófitas; la mayor diversidad de endófitos bacterianos se observó en raíces y hojas de muestras del desierto. Los aislamientos se reconocieron con base en la morfología, la bioquímica y los genes de la secuencia del ARNr 16S. Todos estos aislados indican la capacidad de producir enzimas como amilasa, celulasa, lipasa, catalasa y proteasa en sus descripciones bioquímicas; los análisis también mostraron una indicación significativa de su potencial para producir hormonas de crecimiento vegetal; como su capacidad para disolver el fosfato. En el mundo y en Egipto, somos los primeros en reportar endófitos bacterianos aislados de Senecio glaucus. Este estudio podría ayudar a determinar el papel de las bacterias endófitas en hábitats severos, así como sus posibles aplicaciones en medicina, biorremediación, agricultura e industria

First Report of Endophytic Bacteria Isolated from Senecio glaucus L., Egypt

Microorganisms are naturally associated with plants in several ways. The study was conducted to isolate bacteria endophytes from the internal cells of roots, stems, leaves, and capitula of Senecio glaucus collected from 2 diverse (coastal and desert) habitats in Egypt. A total of 10 endophytic bacteria were obtained from the isolation; the highest diversity of bacterial endophytes was observed in desert samples roots and leaves. The isolates were recognized based on morphology, biochemical and 16S rRNA sequence genes. All isolates indicated the ability for enzyme production as amylase, cellulase, lipase, catalase, and protease in their biochemical descriptions; analyses also gave a significant indication of their potential to produce plant growth hormones, as their ability to dissolve Phosphate. In the world and Egypt, we are the first to report bacterial endophytes isolated from Senecio glaucus. This study could aid in determining the role of endophytic bacteria in severe habitats, as well as their potential applications in medicine, bioremediation, agriculture, and industry

Plant communities along the international coastal highway of Nile delta, Egypt

The construction of highways promotes several changes in the roadside habitat, vegetation, and plant invasion. The present study aimed to describe the plant communities-environment interactions of the international coastal highway from Port-Said to Abu-Qir, northern Nile delta coast of Egypt. A total of 146 species (83 annuals, 4 biennials, and 59 perennials), belonging to 116 genera and 33 families were recorded in 80 stands. The Mediterranean chorotype is the most represented. The cluster analysis of stands yielded four communities. The community I was dominated by Silybum mariannum, community II was dominated by Mesembryanthemum crystalinum, Community III is the largest one and was co-dominated by Hordeum murinum and Senecio glaucus and community IV was co-dominated by Cakile maritima and S. glaucus. Diversity indices showed more richness of communities II and more evenness of community IV. Electric conductivity, porosity, soil texture, Na+, Cl- and bicarbonate showed significant variation among communities. The soil of C. maritima and S. glaucus community was more saline (1.78 ms cm-1). The application of CCA showed that communities II and III were mainly correlated with salinity and organic matter. The present study revealed that roadside habitat of the international coastal highway dominated by different plant communities which correlated with the habitat structure, salinity, and anthropogenic activities. Furthermore, nine invasive species were recorded in this study which could be attributed to the increment of human activities after the construction of the highway

Anatomical investigation of three emergent Cyperus species growing naturally on the canal banks of the Nile delta, Egypt

The genus Cyperus is a large genus with about 600 species, widespread all over the world. The present work contains anatomical descriptions of culms and leaves anatomy of three taxa of Cyprus spp. (Cyperus alopecuroides, C. articulates and C. papyrus). Cyperus spp. were collected from canal banks of Nile Delta. The culms in transverse section of all examined species were triangular except for C. articulates which was circular. The ground tissue differentiated into parenchyma cells with thin walls and small, triangular intercellular spaces, while C. articulates has large hollow pith. Vascular bundles are small, angular and scattered throughout the thin-walled ground tissue. Leaf anatomy of C. alopecuroides and C. papyrus is an example of the isobilateral mesophyll with palisade parenchyma on both sides, enlarged epidermal cells referred to as hing cells found in the middle of the blade. The center of the leaf is occupied by a large vascular bundle surrounded by a bundle sheath.   &nbsp

Primer Informe de Bacterias Endofíticas Aisladas de Senecio glaucus L., Egipto

Los microorganismos están naturalmente asociados con las plantas. El presente experimento se llevó a cabo para aislar bacterias endófitas de las células internas de raíces, tallos, hojas y Tejido capitulear de Senecio glaucus recolectadas en 2 hábitats diversos (costeros y desérticos) de Egipto. Del aislamiento se obtuvieron un total de 10 bacterias endófitas; la mayor diversidad de endófitos bacterianos se observó en raíces y hojas de muestras del desierto. Los aislamientos se reconocieron con base en la morfología, la bioquímica y los genes de la secuencia del ARNr 16S. Todos estos aislados indican la capacidad de producir enzimas como amilasa, celulasa, lipasa, catalasa y proteasa en sus descripciones bioquímicas; los análisis también mostraron una indicación significativa de su potencial para producir hormonas de crecimiento vegetal; como su capacidad para disolver el fosfato. En el mundo y en Egipto, somos los primeros en reportar endófitos bacterianos aislados de Senecio glaucus. Este estudio podría ayudar a determinar el papel de las bacterias endófitas en hábitats severos, así como sus posibles aplicaciones en medicina, biorremediación, agricultura e industria.//Microorganisms are naturally associated with plants in several ways. The study was conducted to isolate bacteria endophytes from the internal cells of roots, stems, leaves, and capitula of Senecio glaucus collected from 2 diverse (coastal and desert) habitats in Egypt. A total of 10 endophytic bacteria were obtained from the isolation; the highest diversity of bacterial endophytes was observed in desert samples roots and leaves. The isolates were recognized based on morphology, biochemical and 16S rRNA sequence genes. All isolates indicated the ability for enzyme production as amylase, cellulase, lipase, catalase, and protease in their biochemical descriptions; analyses also gave a significant indication of their potential to produce plant growth hormones, as their ability to dissolve Phosphate. In the world and Egypt, we are the first to report bacterial endophytes isolated from Senecio glaucus. This study could aid in determining the role of endophytic bacteria in severe habitats, as well as their potential applications in medicine, bioremediation, agriculture, and industry

Proximate Composition, Mineral Content and Secondary Metabolites of Three Medicinal Wild Fagonia Species

Proximate composition of the aerial parts of three Fagonia species (Fagonia arabica L., F. mollis Delile and F. cretica L.) collected from different habitats were analyzed. Macro- and micro-elements as well as some secondary metabolites were estimated. The obtained results revealed that F. creticus contains appreciable levels of nutritive components considering that its nutritional value (351.06 kcal/100g dry wt.) was remarkably higher than that of F. arabica and F. mollis (327.99 and 293.07 kcal/100g dry wt., respectively). The concentration of Na was relatively the highest among the other estimated macroelements in the studied species followed by K, Ca and Mg, respectively while Fe was the highest microelement followed by Cu, Mn and Zn, respectively. The phytochemical composition revealed that methanolic extract of F. creticus was the richest in total alkaloids and flavonoids, while F. arabica found to be the richest in total phenolics and tannins

Chemical Profiling of Significant Antioxidant and Phytotoxic Microwave-Extracted Essential Oil from Araucaria heterophylla Resin

Due to the various hazards of using synthetic chemical compounds in pharmaceutics, agriculture, and industry, scientists and researchers do their best to explore and assess new green natural compounds from natural resources with potent activity. The essential oil (EO) from the resin collected from Araucaria heterophylla Salisb. was extracted by the microwave technique and chemically characterized via GC-MS analysis. Furthermore, the extract EO was assessed for its antioxidant and phytotoxic activities. The EO has 33 compounds, mainly terpenes (98.23%), and the major compounds were α-pinene (62.57%), β-pinene (6.60%), germacrene D (5.88%), and β-caryophyllene (3.56%). The extracted EO showed substantial antioxidant activity, where it showed IC50 values of 142.42 and 118.03 mg L−1 for DPPH and ABTS, respectively. On the other hand, the EO revealed considerable phytotoxicity against the weed Chenopodium murale, where the EO showed IC50 values of 304.0, 230.1, and 147.1 mg L−1, for seed germination, seedling shoot growth, and seedling root growth, respectively. Moreover, the EO showed the same pattern of allelopathic inhibition against the weed Sonchus oleraceus, where it showed IC50 values of 295.7, 224.5, and 106.1 mg L−1, for seed germination, seedling shoot growth, and seedling root growth, respectively. The present study showed that the extraction technique affects the constituents of the EO, particularly the quantitative composition. The EO of A. heterophylla resin also revealed considerable antioxidant and phytotoxic activity against weeds. Therefore, it can be considered a promising natural resource that could be integrated into the weed management approach. However, further study is recommended for deep characterization of their authentic compounds and evaluation of their mode of action(s) on a wide spectrum of weeds

Metabolic profiling of Ochradenus baccatus Delile. utilizing UHPLC-HRESIMS in relation to the in vitro biological investigations

Ochradenus baccatus Delile (Resedaceae) is a desert plant with edible fruits native to the Middle East. Few investigators have reported antibacterial, antiparasitic and anti-cancer activities of the plant. Herein we evaluated the cytotoxic activity of O. baccatus using four cell lines and a zebrafish embryo model. Additionally, liquid chromatography coupled with mass spectroscopy was performed to characterize the extract’s main constituents. The highest cytotoxicity was observed against human cervical adenocarcinoma (HeLa), with CC50 of 39.1 μg/mL and a selectivity index (SI) of 7.23 (p < 0.01). Metabolic analysis of the extract resulted in the annotation of 57 metabolites, including fatty acids, flavonoids, glucosinolates, nitrile glycosides, in addition to organic acids. The extract showed an abundance of hydroxylated fatty acids (16 peaks). Further, 3 nitrile glycosides have been identified for the first time in Ochradenus sp., in addition to 2 glucosinolates. These identified phytochemicals may partially explain the cytotoxic activity of the extract. We propose O. baccatus as a possible safe food source for further utilization to partially contribute to the increasing food demand specially in Saharan countries.Medical University of Lublin | Ref. DS28Universidade de Vigo/CISU

Soil quality and health risk assessment of heavy metals in agricultural areas irrigated with wastewater from Kitchener Drain, Nile Delta, Egypt

Kitchener drain is one of the largest drains in Nile Delta. It discharges water directly into Mediterranean Sea water affecting on the marine environment. Local population uses its water in irrigation and agriculture field along this drain. So it’s important to determine heavy metal content of agricultural soils used this water in irrigation process and assess the hazard and cancer risk on human health living in these areas. Six metals (Fe, Cd, Pb, Ni, Cr and Co) in total and available form were determined in eight geo-referenced soil samples. The order of these metals in soil was as follow; Ni &gt; Cr &gt; Fe &gt; Pb &gt; Cd &gt; Co. The order of these metals in the available form take the sequence of; Fe &gt; Ni &gt; Pb &gt; Cr &gt; Cd &gt; Co.&nbsp; All mean concentrations of metals were exceeding the standard limits of EU, CSQG and AUEC except for cobalt. Mean values of enrichment factors of metals give an indication that the sources of these metals in the environment were from anthropogenic activities. PLI and DC showed considerable degree of contamination in sites 4 &amp; 5. While it showed high degree of contamination in other sites. Hazard quotient from different exposure pathways namely; chemical daily intake (CDI), Dermal contact (DAD) and inhalation (ECinh) and hazard index calculations from metals within different sites were more than one, indicated that there is a chance of non-carcinogenic effects to occur. Also from these pathways, cancer risk (CR) was calculated, which exceed from dermal contact, followed by ingestion and finally from inhalation. Only CR of cobalt showed no risk in the study area when compared with other metal