Growth kinetics and morphological characteristics of the Vero cell line and primary heart cells treated with the plant hormones Auxin, Kinetin, and Gibberellin

Plants produce various hormones that regulate their growth and development. In recent years, much attention has been paid to the effects of these hormones on animal cells. The present work aims to study the influence of three phytohormones: auxin, kinetin and gibberellin on the Vero cell line and primary cardiac cells. These two types of cells are used in the field of virology. The parameters studied are the viability, growth and morphology of the cells. The pH variation and glucose consumption in cell cultures. The results showed that gibberellin stimulates the growth and viability of Vero cells, while kinetin improves the growth of cardiac cells. Auxin also enhances the proliferation of both cell types; but its effect is less significant than that of other phytohormones. The increase in cell growth in the presence of phytohormones was indirectly confirmed by the consumption of glucose and the reduction of pH of the cell culture. This proliferative effect of plant hormones on Vero and Heart cells can be exploited in the field of vaccine production

Genetics of Glioblastoma in Moroccan population: Review of literature

Glioblastoma is the most aggressive malignant type of central nervous system tumors. The literature review revealed that the most common genetic abnormalities in primary and secondary glioblastomas are IDH1 / 2 mutations, p53 mutations, and overexpression of EGFR.To our knowledge, this is the first Moroccan study to provide a global picture of genetic studies performed on Moroccan patients to describe genetic markers and their frequency in our population.An in-depth research in ScienceDirect and Pubmed on glioblastoma articles treated in Morocco, this research is based on the following keywords: glioblastoma in Morocco, primary glioblastoma in Morocco, secondary glioblastoma in Morocco.We found only three research articles on genomic alteration of IDH1 / 2, p53 and EGFR expression. The frequency of these gene mutations in our population was similar to those reported from other populations.Additional molecular studies need to be performed on other genes to describe other genetic markers in the Moroccan population. Keywords: IDH1 and IDH2, P53, EGFR, Primary and secondary glioblastoma Morocc