ASSOCIATION OF OBESITY WITH RS1421085 AND RS9939609 POLYMORPHISMS OF FTO GENE WITH T2DM IN EGYPTIAN FEMALES

Objective: Obesity has been described as a worldwide increasing health problem and risk factor of various disorders including type 2 diabetes mellitus (T2DM). So, our study aim to determine of common variants of fat mass and obesity associated gene polymorphisms rs1421085 and rs 9939609; confers risk of obesity and type 2 diabetic mellitus in Egyptian females.Methods: In this population rs1421085 and rs9939609 polymorphisms of fat mass and obesity (FTO) gene were genotyped in 105 obese patients and 100 healthy controls with ages 14-60 y were collected from Medicine Specialized Hospital, Mansoura University, Egypt during the period between Jul.-Oct. 2016, genotyping of SNPs was performed by restriction fragment length polymorphism (RFLP) assay, fasting blood glucose, homeostasis model assessment of insulin resistance (HOMA IR), body mass index (BMI), waist-to-hip ratio (WHR) lipid profile was determined.Results: There was the significantly higher frequency of the AA compared to controls p=0.0001) of genotypers9939609. Also, cases have shown a significantly higher frequency of the C allele, p<0.00001) of rs1421085 genotype polymorphisms increased the risks of obesity. On the other hand, there were no significant correlations between genotypes and obesity-related (anthropometric body composition) parameters. Only the fasting blood glucose was significantly higher in the TA p=0.004).Conclusion: The FTO rs9939609 and rs1421085 single nucleotide polymorphisms (SNPs) was associated with increased risk of obesity in type 2 diabetic populations on Egyptian females

VASCULAR ENDOTHELIAL GROWTH FACTOR VEGF G/C 405 AND C/A 2578 GENE POLYMORPHISMS IN CASES WITH PRE-ECLAMPSIA

Vascular endothelial growth factor gene plays a crucial role in physiological vasculogenesis and vascular permeability and has been implicated in the pathogenesis of pre-eclampsia (PE). VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. This study carried on 170 pregnant women. Out of them, 100 cases with PE. Their mean age ± SD was 25.28 ± 4.59 years. Seventy nine (79%) cases had mild PE and 21 (21%) cases had severe PE. The other 70 subjects were clinically healthy pregnant women with complete successful pregnancy and no PE. VEGF C 405 G and C 2578 A polymorphisms were studied using PCR and RFLP. We found that PE among Egyptian women was strongly associated with pre-eclamptic mutations related to VEGF C 405 G and C 2578 A gene polymorphisms. There were statistically significant low frequencies of wild homozygous genotype CC and high frequencies of heterozygous mutant genotypes CG & CA and homozygous mutant genotypes GG & AA in PE cases compared to controls. The frequency of cases carrying the homozygous mutant genotype GG was higher among severe pre-eclamptic cases compared to mild pre-eclamptic cases. In addition, there were statistically significant high frequencies in PE cases compared to controls of mutant G and mutant A alleles

Association of cytokine gene polymorphisms with psoriasis in cases from the Nile Delta of Egypt

Background: Psoriasis is a chronic inflammatory skin disease with an immunogenetic background. This work was planned to check for the association of polymorphisms related to cytokine genes TNF-α-308 (G/A), IL-10 -1082 (G/A), IL-6 -174 (G/C), and IL-1Ra (VNTR) with psoriasis in cases from Egypt. Materials and Methods: This work included 46 cases with psoriasis recruited from the Dermatology Departments, University Hospitals, Nile Delta region of Egypt. They included 14 males and 32 females with an age mean ± SD of 46.68 ± 12.16 years and range of 15-70 years. Their genotypes were compared to 98 healthy controls of matched age and sex from the same locality. Genotyping was done through deoxyribonucleic acid amplification using PCR with sequence specific primers for polymorphic alleles. Results: Compared to controls, cases showed significant higher frequency of certain genotypes including IL-6 -174 CC (P < 0.001, OR = 6.7), IL-10 -1082 GG ( P < 0.05, OR = 5.1), and TNF-α-308 GG ( P < 0.05, OR = 3.7). TNF-α-308 GG and IL-10 -1082 GG genotypes were higher among cases with plaque subtype of moderate severity. Combined heterozygosity for IL-10 GA, IL-6 GC with TNF GA showed a significant low frequency among studied cases. Conclusion: Genetic polymorphisms related to IL6, IL10, and TNF-α genes showed a particular pattern of association with psoriasis that may have a potential impact on disease counseling and management