Reflectivity and microhardness of sulfide minerals as genetic information source (case study: pyrite and arsenopyrite)

Reflectivity and microhardness of pyrite and arsenopyrite of black shale gold-ore deposits in Chertovo Koryto (Patom upland) were studied. It was found that sulfides of different generations are characterized by different values of above-mentioned parameters which is associated mechanical and isomorphic impurities

Peculiarities of pyrrhotite mineralization in the Chertovo Koryto deposit (Patom Uplands)

Pyrrhotite mineralization of black shale gold-ore deposit in the central part of Chertovo Koryto (Patom Uplands) was studied. It was found that pyrrhotite consists of three generations, containing hexagonal and monoclinic modifications in various proportions. The increase in the monoclinic modification from the earlier to later generation was observed

Reflectivity and microhardness of sulfide minerals as genetic information source (case study: pyrite and arsenopyrite)

Reflectivity and microhardness of pyrite and arsenopyrite of black shale gold-ore deposits in Chertovo Koryto (Patom upland) were studied. It was found that sulfides of different generations are characterized by different values of above-mentioned parameters which is associated mechanical and isomorphic impurities

Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

Laccase is one of the oldest known and intensively studied fungal enzymes capable of oxidizing recalcitrant lignin-resembling phenolic compounds. It is currently well established that fungal genomes almost always contain several non-allelic copies of laccase genes (laccase multigene families); nevertheless, many aspects of laccase multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed evolutionary analysis of the sensu stricto laccase genes (CAZy – AA1_1) from fungi of the Polyporales order. The conducted analysis provides a better understanding of the Polyporales laccase multigenicity and allows for the systemization of the individual features of different laccase isozymes. In addition, we provide a comparison of the biochemical and catalytic properties of the four laccase isozymes from Trametes hirsuta and suggest their functional diversification within the multigene family

Ribosomal DNA as DAMPs Signal for MCF7 Cancer Cells

Introduction: The cell free ribosomal DNA (cf-rDNA) is accrued in the total pool of cell free DNA (cfDNA) in some non-cancer diseases and demonstrates DAMPs characteristics. The major research questions: (1) How does cell free rDNA content change in breast cancer; (2) What type of response in the MCF7 breast cancer cells is caused by cf-rDNA; and (3) What type of DNA sensors (TLR9 or AIM2) is stimulated in MCF7 in response to the action of cf-rDNA?Materials and Methods: CfDNA and gDNA were isolated from the blood plasma and the cells derived from 38 breast cancer patients and 20 healthy female controls. The rDNA content in DNA was determined using non-radioactive quantitative hybridization. In order to explore the rDNA influence on MCF7 breast cancer cells, the model constructs (GC-DNAs) were applied: pBR322-rDNA plasmid (rDNA inset 5836 bp long) and pBR322 vector. ROS generation, DNA damage, cell cycle, expression of TLR9, AIM2, NF-kB, STAT3, and RNA for 44 genes affecting the cancer cell viability were evaluated. The methods used: RT-qPCR, fluorescent microscopy, immunoassay, flow cytometry, and siRNA technology.Results: The ratio R = cf-rDNA/g-rDNA for the cases was higher than for the controls (median 3.4 vs. 0.8, p < 10−8). In MCF7, GC-DNAs induce a ROS burst, DNA damage response, and augmentation of NF-kB and STAT3 activity. The number of the apoptotic cells decreases, while the number of cells with an instable genome (G2/M– arrest, micronuclei) increase. Expression of anti-apoptotic genes (BCL2, BCL2A1, BCL2L1, BIRC3, MDM2) is elevated, while expression of pro-apoptotic genes (BAX, BID, BAD, PMAIP1, BBC3) is lowered. The cells response for pBR322-rDNA is much more intense and develops much faster, than response for pBR322, and is realized through activation of TLR9- MyD88 - NF-kB- signaling. This difference in response speed is owing to the heightened oxidability of pBR322-rDNA and better ability to penetrate the cell. Induction of TLR9 expression in MCF7 is followed by blocking AIM2 expression.Conclusion: (1) Ribosomal DNA accumulates in cfDNA of breast cancer patients; (2) Cell free rDNA induce DNA damage response and stimulates cells survival, including cells with an instable genome; (3) Cell free rDNA triggers TLR9- MyD88- NF-kB- signaling, with significantly repressing the expression of AIM2

Scientific Conferences as Way to Develop Students’ Foreign Language Communicative Competence

The issues of the student engagement in science-related activities and the development of students’ language communicative competence are especially relevant in a technical university, where due to the prevailing of the Sciences, the professional communicative competence has become increasingly vital. The goal of this article is to examine how interdisciplinary scientific conferences for students held in foreign languages can foster the foreign language communicative competence of students. In the article, we present the definition and the three basic models of communicative competence. A method of pedagogical observation is used that represents comprehension and analysis of goal-oriented preparation of students for practical scientific conferences. We reveal the fact that interdisciplinary scientific conferences for students held in foreign languages allow educators to foster the foreign language communicative competence of students and deepen their knowledge in professional area, as well as to equip them with research skills since students’ participation in the conferences increases their attention and focus, motivates them to practice critical thinking skills of high level

Scientific Conferences as Way to Develop Students’ Foreign Language Communicative Competence

The issues of the student engagement in science-related activities and the development of students’ language communicative competence are especially relevant in a technical university, where due to the prevailing of the Sciences, the professional communicative competence has become increasingly vital. The goal of this article is to examine how interdisciplinary scientific conferences for students held in foreign languages can foster the foreign language communicative competence of students. In the article, we present the definition and the three basic models of communicative competence. A method of pedagogical observation is used that represents comprehension and analysis of goal-oriented preparation of students for practical scientific conferences. We reveal the fact that interdisciplinary scientific conferences for students held in foreign languages allow educators to foster the foreign language communicative competence of students and deepen their knowledge in professional area, as well as to equip them with research skills since students’ participation in the conferences increases their attention and focus, motivates them to practice critical thinking skills of high level

Automated Acid Rock Drainage Indexing from Drill Core Imagery

The automated classification of acid rock drainage (ARD) potential developed in this study is based on a manual ARD Index (ARDI) logging code. Several components of the ARDI require accurate identification of sulfide minerals that hyperspectral drill core scanning technologies cannot yet report. To overcome this, a new methodology was developed that uses red⁻green⁻blue (RGB) true color images generated by Corescan® to determine the presence or absence of sulfides using supervised classification. The output images were then recombined with Corescan® visible to near infrared-shortwave infrared (VNIR-SWIR) mineral classifications to obtain information that allowed an automated ARDI (A-ARDI) assessment to be performed. To test this, A-ARDI estimations and the resulting acid-forming potential classifications for 22 drill core samples obtained from a porphyry Cu⁻Au deposit were compared to ARDI classifications made from manual observations and geochemical and mineralogical analyses. Results indicated overall agreement between automated and manual ARD potential classifications and those from geochemical and mineralogical analyses. Major differences between manual and automated ARDI results were a function of differences in estimates of sulfide and neutralizer mineral concentrations, likely due to the subjective nature of manual estimates of mineral content and automated classification image resolution limitations. The automated approach presented here for the classification of ARD potential offers rapid and repeatable outcomes that complement manual and analyses derived classifications. Methods for automated ARD classification from digital drill core data represent a step-change for geoenvironmental management practices in the mining industry

Types of Void Space in the Bazhenov Reservoir Rocks

The deposits of the Bazhenov formation are a unique reservoir of unconventional oil reserves in Western Siberia. They contain both solid organic matter (kerogen) and liquid light oil. The successful development of these hydrocarbons is largely determined by the adequacy of the void space models. The aim of the study is to identify the types of void space in the sediments of the Bazhenov formation and to identify the distribution patterns across the section of the researched wells. The void space was studied by electron and optical microscopy, and the mineral composition of the rocks was determined by X-ray diffraction analysis. The deposits of the Bazhenov productive formation in the territory of Western Siberia are represented by a wide complex of lithotypes, including various kinds of silicites, carbonate, clay rocks, and mixtites. The reservoir space in the reservoir rocks of the Bazhenov formation is a complex and hierarchically subordinated system, which includes voids and fractures of various sizes, configurations, and genesis. The void space of the Bazhenov formation is characterized by a fairly high degree of spatial heterogeneity, which is controlled by lithological, facies, and tectonic factors, as well as the direction of catagenetic processes