The role of light desynchronosis in the development of stress-induced aging

The long-term change of the light mode for three months – light desynchronosis, disturbs the rhythm of the signals received from the external pacemaker. As a result of the study, it was found that a long-term change in the light mode and a violation of the rhythmicity of signals received from an external pacemaker contributes to the activation of ROS formation as triggers for bioenergetic processes in the cell. At the same time, changing the light mode disrupts the balance of oxygen in the cell and this is a provoking factor for the stress of the antioxidant cell system. The resulting tissue hypoxia in chronic light desynchronosis disrupts the bioenergetic potential of the cell, contributing to the development of pathophysiological processes and the death of neurons. Therefore, a violation of the balance of the pro-oxidant and anti-oxidant systems leads to destructive processes in the brain. A significant change in the concentration of the neurotrohic markers indicates destructive processes in the brain tissues. Summarizing the above, we conclude that light desynchronosis is directly involved in the ROS-dependent stress-induced aging of brain cells and in that way, to the progression of processes that lead to aging of the body

Genetic Deletion of Trace-Amine Associated Receptor 9 (TAAR9) in Rats Leads to Decreased Blood Cholesterol Levels

In the last two decades, interest has grown significantly in the investigation of the role of trace amines and their receptors in mammalian physiology and pathology. Trace amine-associated receptor 9 (TAAR9) is one of the least studied members of this receptor family with unidentified endogenous ligands and an unknown role in the central nervous system and periphery. In this study, we generated two new TAAR9 knockout (TAAR9-KO) rat strains by CRISPR-Cas9 technology as in vivo models to evaluate the role of TAAR9 in mammalian physiology. In these mutant rats, we performed a comparative analysis of a number of hematological and biochemical parameters in the blood. Particularly, we carried out a complete blood count, erythrocyte osmotic fragility test, and screening of a panel of basic biochemical parameters. No significant alterations in any of the hematological and most biochemical parameters were found between mutant and WT rats. However, biochemical studies revealed a significant decrease in total and low-density lipoprotein cholesterol levels in the blood of both strains of TAAR9-KO rats. Such role of TAAR9 in cholesterol regulation not only brings a new understanding of mechanisms and biological pathways of lipid exchange but also provides a new potential drug target for disorders involving cholesterol-related pathology, such as atherosclerosis