3 research outputs found
Fatigue Profiles in Patients with Multiple Sclerosis are Based on Severity of Fatigue and not on Dimensions of Fatigue
Fatigue related to Multiple Sclerosis (MS) is considered a multidimensional symptom, manifesting in several dimensions such as physical, cognitive, and psychosocial fatigue. This study investigated in 264 patients with severe primary MS-related fatigue (median MS duration 6.8 years, mean age 48.1 years, 75% women) whether subgroups can be distinguished based on these dimensions. Subsequently, we tested whether MS-related
Aqueous humor proteome of primary open angle glaucoma: A combined dataset of mass spectrometry studies
Analysis of the proteins of the aqueous humor can help to
elucidate the complex pathogenesis of primary open angle
glaucoma. Thanks to advances in liquid chromatography tandem mass spectrometry (LC-MS/MS) it is now possible to
identify hundreds of proteins in individual aqueous humor
samples without the need to pool samples. We performed
a systematic literature search to find publications that performed LC-MS/MS on aqueous humor samples of glaucoma
patients and of non-glaucomatous controls. Of the seven
publications that we found, we obtained the raw data of
three publications. These three studies used glaucoma patients that were clinically similar (i.e. undergoing glaucoma
filtration surgery) which prompted us to reanalyse and combine their data. Raw data of each study were analysed separately with the latest version of MaxQuant (version v1.6.11.0).
Outcome files were exported to Microsoft Excel. Samples belonging to the same patient were averaged to obtain peptide
expression values per individual. We compared the overlap of identified proteins using the VLOOKUP function of Excel
and a publicly available Venn diagram software. For the peptide sequences that can belong to multiple proteins (usually
of the same protein family), we initially included all possibly identified proteins. This ensured that we would not miss
a potential overlap between the studies due to differences in
identified peptide counts. Next, of those peptides of which
we compared multiple proteins, only one unique protein was
included in our analysis i.e. either the protein overlapping
bet
The aqueous humor proteome of primary open angle glaucoma: An extensive review
Background: We reviewed the literature on the aqueous humor (AH) proteome of primary open angle glaucoma (POAG) patients in order to obtain deeper insight into the pathophysiology of POAG. Methods: We searched Pubmed and Embase up to May 2019 for studies that compared AH protein composition between POAG (cases) and cataract (controls). Untargeted studies (measuring the whole proteome, by LC-MS/MS) were divided into two subgroups depending on the type of surgery during which POAG AH was collected: glaucoma filtration surgery (subgroup 1) or cataract surgery (subgroup 2). We reanalyzed the raw data (subgroup 1) or combined the reported data (subgroup 2) to perform GO enrichment (GOrilla) and pathway analysis (Pathvisio). Results: Out of 93 eligible proteomic studies, seven were untargeted studies that identified 863 AH proteins. We observed 73 differentially expressed proteins in subgroup 1 and 87 differentially expressed proteins in subgroup 2. Both subgroups were characterized by activation of the acute immune response, dysregulation of folate metabolism and dysregulation of the selenium micronutrient network. For subgroup 1 but not for subgroup 2, proteins of the complement system were significantly enriched. Conclusion: AH proteome of POAG patients shows strong activation of the immune system. In addition, analysis suggests dysregulation of folate metabolism and dysregulation of selenium as underlying contributors. In view of their glaucoma surgery, POAG patients of subgroup 1 most likely are progressive whereas POAG patients in subgroup 2 most likely have stable POAG. The proteome difference between these subgroups suggests that the complement system plays a role in POAG progression