Aggression, anxiety and vocalizations in animals: GABA A and 5-HT anxiolytics

A continuing challenge for preclinical research on anxiolytic drugs is to capture the affective dimension that characterizes anxiety and aggression, either in their adaptive forms or when they become of clinical concern. Experimental protocols for the preclinical study of anxiolytic drugs typically involve the suppression of conditioned or unconditioned social and exploratory behavior (e.g., punished drinking or social interactions) and demonstrate the reversal of this behavioral suppression by drugs acting on the benzodiazepine-GABA A complex. Less frequently, aversive events engender increases in conditioned or unconditioned behavior that are reversed by anxiolytic drugs (e.g., fear-potentiated startle). More recently, putative anxiolytics which target 5-HT receptor subtypes produced effects in these traditional protocols that often are not systematic and robust. We propose ethological studies of vocal expressions in rodents and primates during social confrontations, separation from social companions, or exposure to aversive environmental events as promising sources of information on the affective features of behavior. This approach focusses on vocal and other display behavior with clear functional validity and homology. Drugs with anxiolytic effects that act on the benzodiazepine-GABA A receptor complex and on 5-HT 1A receptors systematically and potently alter specific vocalizations in rodents and primates in a pharmacologically reversible manner; the specificity of these effects on vocalizations is evident due to the effectiveness of low doses that do not compromise other physiological and behavioral processes. Antagonists at the benzodiazepine receptor reverse the effects of full agonists on vocalizations, particularly when these occur in threatening, startling and distressing contexts. With the development of antagonists at 5-HT receptor subtypes, it can be anticipated that similar receptor-specificity can be established for the effects of 5-HT anxiolytics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46351/1/213_2005_Article_BF02245590.pd

Eddy Current Detection of the Martensitic Transformation in AISI304 Induced upon Cryogenic Cutting

The combination of a hard subsurface layer and a ductile component core is advantageous for many applications. Steels are often heat treated to create such a hardened subsurface, which is both time- and energy-consuming. It is of great advantage to create a hardened subsurface directly within the machining process, as the production line of most components includes such a process to produce the desired geometric dimensions and surface quality. To achieve a martensitic subsurface layer within the machining process, cryogenic, external turning using a metastable AISI304 austenitic steel is used herein. Herein eddy current testing and the analysis of higher harmonics are used for the detection of the ferromagnetic, martensitic phase in the parent austenite. A good correlation is found between the martensite content and the amplitude of the signals measured. Therefore, eddy current testing is considered as a suitable real-time, nondestructive testing method, which forms the basis for the generation of a tailored, deformation-induced martensitic subsurface layer during external turning