Acyl coenzyme a: 2-acyl-sn-glycerol-3-phosphate acyltransferase activity in rat liver microsomes

2-Acylglycerol-3-phosphate can be acylated by acyl-CoA in the presence of microsomal preparations from rat liver. With optimal amounts of substrate, the acyltransferase reaction to the 1-position occurred at about one tenth the rate observed for the 2-position. Oleate was esterified more rapidly than palmitate or stearate under the conditions used. The enzymic activity catalyzing esterification of the primary hydroxyl seems to be different from that for the secondary hydroxyl.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33532/1/0000031.pd