A searchable image resource of Drosophila GAL4-driver expression patterns with single neuron resolution

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 74,000 such adult central nervous systems. An anticipated use of this resource is to bridge the gap between neurons identified by electron or light microscopy. Identifying individual neurons that make up each GAL4 expression pattern improves the prediction of split-GAL4 combinations targeting particular neurons. To this end we have made the images searchable on the NeuronBridge website. We demonstrate the potential of NeuronBridge to rapidly and effectively identify neuron matches based on morphology across imaging modalities and datasets

Aggregating expert judgement

In a paper written some 25 years ago, I distinguished three contexts in which one might wish to combine expert judgements of uncertainty: the expert problem, the group decision problem and the textbook problem. Over the intervening years much has been written on the first two, which have the focus of a single decision context, but little on the third, though the closely related field of meta-analysis has developed considerably. With many developments in internet technology, particularly in relation to interactivity and communication, the textbook problem is gaining in importance since data and expert judgements can be made available over the web to be used by many different individuals to shape their own beliefs in many different contexts. Moreover, applications such as web-based decision support, e-participation and e-democracy are making algorithmic ‘solutions’ to the group decision problem attractive, despite many results showing we know that such solutions are, at best, rare and, at worst, illusory. In this paper I survey developments since my earlier paper and note some unresolved issues. Then I turn to how expert judgement might be used within web-based group decision support, as well as in e-participation and e-democracy contexts. The latter points to a growing importance of the textbook problem and suggests that Cooke’s principles for scientific reporting of expert judgement studies may need enhancing for such studies to be used by a wider audience

The Arabidopsis Nuclear Pore and Nuclear Envelope

The nuclear envelope is a double membrane structure that separates the eukaryotic cytoplasm from the nucleoplasm. The nuclear pores embedded in the nuclear envelope are the sole gateways for macromolecular trafficking in and out of the nucleus. The nuclear pore complexes assembled at the nuclear pores are large protein conglomerates composed of multiple units of about 30 different nucleoporins. Proteins and RNAs traffic through the nuclear pore complexes, enabled by the interacting activities of nuclear transport receptors, nucleoporins, and elements of the Ran GTPase cycle. In addition to directional and possibly selective protein and RNA nuclear import and export, the nuclear pore gains increasing prominence as a spatial organizer of cellular processes, such as sumoylation and desumoylation. Individual nucleoporins and whole nuclear pore subcomplexes traffic to specific mitotic locations and have mitotic functions, for example at the kinetochores, in spindle assembly, and in conjunction with the checkpoints. Mutants of nucleoporin genes and genes of nuclear transport components lead to a wide array of defects from human diseases to compromised plant defense responses. The nuclear envelope acts as a repository of calcium, and its inner membrane is populated by functionally unique proteins connected to both chromatin and—through the nuclear envelope lumen—the cytoplasmic cytoskeleton. Plant nuclear pore and nuclear envelope research—predominantly focusing on Arabidopsis as a model—is discovering both similarities and surprisingly unique aspects compared to the more mature model systems. This chapter gives an overview of our current knowledge in the field and of exciting areas awaiting further exploration