Exact controllability for wave equation on general quantum graphs with non-smooth controls

In this paper we study the exact controllability problem for the wave equation on a finite metric graph with the Kirchhoff-Neumann matching conditions. Among all vertices and edges we choose certain active vertices and edges, and give a constructive proof that the wave equation on the graph is exactly controllable if $H^1(0,T)'$ Neumann controllers are placed at the active vertices and $L^2(0,T)$ Dirichlet controllers are placed at the active edges. The proofs for the shape and velocity controllability are purely dynamical, while the proof for the exact controllability utilizes both dynamical and moment method approaches. The control time for this construction is determined by the chosen orientation and path decomposition of the graph

The X-ray Reflectors in the Nucleus of the Seyfert Galaxy NGC 1068

(abridged) Based on observations of the Seyfert nucleus in NGC1068 with ASCA, RXTE and BeppoSAX, we report the discovery of a flare (increase in flux by a factor of ~1.6) in the 6.7 keV Fe K line component between observations obtained 4 months apart, with no significant change in the other (6.21, 6.4, and 6.97 keV) Fe K_alpha line components. During this time, the continuum flux decreased by ~20%. The RXTE spectrum requires an Fe K absorption edge near 8.6 keV (Fe XXIII - XXV). The spectral data indicate that the 2-10 keV continuum emission is dominated (~2/3 of the luminosity) by reflection from a previously unidentified region of warm, ionized gas located <~ 0.2 pc from the AGN. The remaining ~1/3 of the observed X-ray emission is reflected from optically thick, neutral gas. The inferred properties of the warm reflector (WR) are: size (diameter) ~ 10^{5.5} /cm3, ionization parameter xi approx 10^{3.5} erg cm/s, and covering fraction 0.003 (L_0/10^{43.5} erg/s)^{-1} < (Omega/4 pi) < 0.024 (L_0/10^{43.5})^{-1}, where L_0 is the intrinsic 2-10 keV X-ray luminosity of the AGN. We suggest that the WR gas is the source of the (variable) 6.7 keV Fe line emission, and the 6.97 keV Fe line emission. The 6.7 keV line flare is assumed to be due to an increase in the emissivity of the WR gas from a decrease (by 20-30%) in L_0. The properties of the WR are most consistent with an intrinsically X-ray weak AGN with L_0 approx 10^{43.0} erg/s. The optical and UV emission that scatters from the WR into our line of sight is required to suffer strong extinction, which can be reconciled if the line-of-sight skims the outer surface of the torus. Thermal bremsstrahlung radio emission from the WR may be detectable in VLBA radio maps of the NGC 1068 nucleus.Comment: 39 pages (9 postscript figures) AASTEX, ApJ, accepte

Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans

Rabies kills many people throughout the developing world every year. The murine monoclonal antibody (mAb) 62-71-3 was recently identified for its potential application in rabies postexposure prophylaxis (PEP). The purpose here was to establish a plant-based production system for a chimeric mouse-human version of mAb 62-71-3, to characterize the recombinant antibody and investigate at a molecular level its interaction with rabies virus glycoprotein. Chimeric 62-71-3 was successfully expressed in Nicotiana benthamiana. Glycosylation was analyzed by mass spectroscopy; functionality was confirmed by antigen ELISA, as well as rabies and pseudotype virus neutralization. Epitope characterization was performed using pseudotype virus expressing mutagenized rabies glycoproteins. Purified mAb demonstrated potent viral neutralization at 500 IU/mg. A critical role for antigenic site I of the glycoprotein, as well as for two specific amino acid residues (K226 and G229) within site I, was identified with regard to mAb 62-71-3 neutralization. Pseudotype viruses expressing glycoprotein from lyssaviruses known not to be neutralized by this antibody were the controls. The results provide the molecular rationale for developing 62-71-3 mAb for rabies PEP; they also establish the basis for developing an inexpensive plant-based antibody product to benefit low-income families in developing countries.—Both, L., van Dolleweerd, C., Wright, E., Banyard, A. C., Bulmer-Thomas, B., Selden, D., Altmann, F., Fooks, A. R., Ma, J. K.-C. Production, characterization, and antigen specificity of recombinant 62-71-3, a candidate monoclonal antibody for rabies prophylaxis in humans

Correlating electroconvulsive therapy response to electroencephalographic markers: Study protocol

INTRODUCTION: Electroconvulsive therapy (ECT) is an effective intervention for patients with major depressive disorder (MDD). Despite longstanding use, the underlying mechanisms of ECT are unknown, and there are no objective prognostic biomarkers that are routinely used for ECT response. Two electroencephalographic (EEG) markers, sleep slow waves and sleep spindles, could address these needs. Both sleep microstructure EEG markers are associated with synaptic plasticity, implicated in memory consolidation, and have reduced expression in depressed individuals. We hypothesize that ECT alleviates depression through enhanced expression of sleep slow waves and sleep spindles, thereby facilitating synaptic reconfiguration in pathologic neural circuits. METHODS: Correlating ECT Response to EEG Markers (CET-REM) is a single-center, prospective, observational investigation. Wireless wearable headbands with dry EEG electrodes will be utilized for at-home unattended sleep studies to allow calculation of quantitative measures of sleep slow waves (EEG SWA, 0.5-4 Hz power) and sleep spindles (density in number/minute). High-density EEG data will be acquired during ECT to quantify seizure markers. DISCUSSION: This innovative study focuses on the longitudinal relationships of sleep microstructure and ECT seizure markers over the treatment course. We anticipate that the results from this study will improve our understanding of ECT

Monoclonal Antibodies to Distinct Regions of Human Myelin Proteolipid Protein Simultaneously Recognize Central Nervous System Myelin and Neurons of Many Vertebrate Species

Myelin proteolipid protein (PLP), the major protein of mammalian CNS myelin, is a member of the proteolipid gene family (pgf). It is an evolutionarily conserved polytopic integral membrane protein and a potential autoantigen in multiple sclerosis (MS). To analyze antibody recognition of PLP epitopes in situ, monoclonal antibodies (mAbs) specific for different regions of human PLP (50–69, 100–123, 139–151, 178–191, 200–219, 264–276) were generated and used to immunostain CNS tissues of representative vertebrates. mAbs to each region recognized whole human PLP on Western blots; the anti-100–123 mAb did not recognize DM-20, the PLP isoform that lacks residues 116–150. All of the mAbs stained fixed, permeabilized oligodendrocytes and mammalian and avian CNS tissue myelin. Most of the mAbs also stained amphibian, teleost, and elasmobranch CNS myelin despite greater diversity of their pgf myelin protein sequences. Myelin staining was observed when there was at least 40% identity of the mAb epitope and known pgf myelin proteins of the same or related species. The pgf myelin proteins of teleosts and elasmobranchs lack 116–150; the anti-100–123 mAb did not stain their myelin. In addition to myelin, the anti-178– 191 mAb stained many neurons in all species; other mAbs stained distinct neuron subpopulations in different species. Neuronal staining was observed when there was at least approximately 30% identity of the PLP mAb epitope and known pgf neuronal proteins of the same or related species. Thus, anti-human PLP epitope mAbs simultaneously recognize CNS myelin and neurons even without extensive sequence identity. Widespread anti-PLP mAb recognition of neurons suggests a novel potential pathophysiologic mechanism in MS patients, i.e., that anti-PLP antibodies associated with demyelination might simultaneously recognize pgf epitopes in neurons, thereby affecting their functions

Scale-Invariant Gravity: Geometrodynamics

We present a scale-invariant theory, conformal gravity, which closely resembles the geometrodynamical formulation of general relativity (GR). While previous attempts to create scale-invariant theories of gravity have been based on Weyl's idea of a compensating field, our direct approach dispenses with this and is built by extension of the method of best matching w.r.t scaling developed in the parallel particle dynamics paper by one of the authors. In spatially-compact GR, there is an infinity of degrees of freedom that describe the shape of 3-space which interact with a single volume degree of freedom. In conformal gravity, the shape degrees of freedom remain, but the volume is no longer a dynamical variable. Further theories and formulations related to GR and conformal gravity are presented. Conformal gravity is successfully coupled to scalars and the gauge fields of nature. It should describe the solar system observations as well as GR does, but its cosmology and quantization will be completely different.Comment: 33 pages. Published version (has very minor style changes due to changes in companion paper

The Murray collection of pre-antibiotic era Enterobacteriacae: a unique research resource.

Studies of historical isolates inform on the evolution and emergence of important pathogens and phenotypes, including antimicrobial resistance. Crucial to studying antimicrobial resistance are isolates that predate the widespread clinical use of antimicrobials. The Murray collection of several hundred bacterial strains of pre-antibiotic era Enterobacteriaceae is an invaluable resource of historical strains from important pathogen groups. Studies performed on the Collection to date merely exemplify its potential, which will only be realised through the continued effort of many scientific groups. To enable that aim, we announce the public availability of the Murray collection through the National Collection of Type Cultures, and present associated metadata with whole genome sequence data for over half of the strains. Using this information we verify the metadata for the collection with regard to subgroup designations, equivalence groupings and plasmid content. We also present genomic analyses of population structure and determinants of mobilisable antimicrobial resistance to aid strain selection in future studies. This represents an invaluable public resource for the study of these important pathogen groups and the emergence and evolution of antimicrobial resistance

Chromosome Congression by Kinesin-5 Motor-Mediated Disassembly of Longer Kinetochore Microtubules

During mitosis, sister chromatids congress to the spindle equator and are subsequently segregated via attachment to dynamic kinetochore microtubule (kMT) plus-ends. A major question is how kMT plus-end assembly is spatially regulated to achieve chromosome congression. Here we find in budding yeast that the widely-conserved kinesin-5 sliding motor proteins, Cin8p and Kip1p, mediate chromosome congression by suppressing kMT plus-end assembly of longer kMTs. Of the two, Cin8p is the major effector and its activity requires a functional motor domain. In contrast, the depolymerizing kinesin-8 motor Kip3p plays a minor role in spatial regulation of yeast kMT assembly. Our analysis identified a model where kinesin-5 motors bind to kMTs, move to kMT plus ends, and upon arrival at a growing plus-end promote net kMT plus-end disassembly. In conclusion, we find that length-dependent control of net kMT assembly by kinesin-5 motors yields a simple and stable self-organizing mechanism for chromosome congression

The Ninth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the SDSS-III Baryon Oscillation Spectroscopic Survey

The Sloan Digital Sky Survey III (SDSS-III) presents the first spectroscopic data from the Baryon Oscillation Spectroscopic Survey (BOSS). This ninth data release (DR9) of the SDSS project includes 535,995 new galaxy spectra (median z=0.52), 102,100 new quasar spectra (median z=2.32), and 90,897 new stellar spectra, along with the data presented in previous data releases. These spectra were obtained with the new BOSS spectrograph and were taken between 2009 December and 2011 July. In addition, the stellar parameters pipeline, which determines radial velocities, surface temperatures, surface gravities, and metallicities of stars, has been updated and refined with improvements in temperature estimates for stars with T_eff<5000 K and in metallicity estimates for stars with [Fe/H]>-0.5. DR9 includes new stellar parameters for all stars presented in DR8, including stars from SDSS-I and II, as well as those observed as part of the SDSS-III Sloan Extension for Galactic Understanding and Exploration-2 (SEGUE-2). The astrometry error introduced in the DR8 imaging catalogs has been corrected in the DR9 data products. The next data release for SDSS-III will be in Summer 2013, which will present the first data from the Apache Point Observatory Galactic Evolution Experiment (APOGEE) along with another year of data from BOSS, followed by the final SDSS-III data release in December 2014.Comment: 9 figures; 2 tables. Submitted to ApJS. DR9 is available at http://www.sdss3.org/dr