Effect of Fabrication Technique on the Microgap of CAD/CAM Cobalt-Chrome and Zirconia Abutments on a Conical Connection Implant: An In Vitro Study.

The aim of this in vitro study was to investigate the microgaps at the implant-abutment interface when zirconia (Zr) and CAD/CAM or cast Co-Cr abutments were used. METHODS Sixty-four conical connection implants and their abutments were divided into four groups (Co-Cr (milled, laser-sintered and castable) and Zirconia (milled)). After chewing simulation (300,000 cycles, under 200 N loads at 2 Hz at a 30° angle) and thermocycling (10,000 cycles, 5 to 50 °C, dwelling time 55 s), the implant-abutment microgap was measured 14 times at each of the four anatomical aspects on each specimen by using a scanning electron microscope (SEM). Kruskal-Wallis and pair-wise comparison were used to analyze the data (α = 0.05). RESULTS The SEM analysis revealed smaller microgaps with Co-Cr milled abutments (0.69-8.39 μm) followed by Zr abutments (0.12-6.57 μm), Co-Cr sintered (7.31-25.7 μm) and cast Co-Cr (1.68-85.97 μm). Statistically significant differences were found between milled and cast Co-Cr, milled and laser-sintered Co-Cr, and between Zr and cast and laser-sintered Co-Cr (p < 0.05). CONCLUSIONS The material and the abutment fabrication technique affected the implant-abutment microgap magnitude. The Zr and the milled Co-Cr presented smaller microgaps. Although the CAD/CAM abutments presented the most favorable values, all tested groups had microgaps within a range of 10 to 150 μm

Linear dimensional change of heat-cured acrylic resin complete dentures after reline and rebase

published_or_final_versionDentistryMasterMaster of Dental Surger

Oral health and quality of life after intensity-modulated head and neck radiotherapy for nasopharyngeal carcinoma

published_or_final_versionDentistryDoctoralDoctor of Philosoph

Assessing Enamel Wear of Monolithic Ceramics With Micro-CT and Intra-oral Scanner

ABSTRACT: Objective: This in vitro study aimed to investigate enamel wear against 3 monolithic ceramics using 2 methods of measurement. Methods: Three groups of standard flat monolithic ceramic specimens including zirconia-reinforced lithium silicate glass (Vita Suprinity, VITA Zahnfabrik), yttria-stabilised tetragonal zirconia (Lava Esthetic Zirconia), and lithium disilicate glass (IPS e.max Press, Ivoclar Vivadent) were prepared, with human enamel used as the control group. Each specimen was subjected to the 2-body wear test at 49 N for 250,000 cycles. Enamel antagonists were evaluated with micro-computed tomography (CT) and intra-oral scanner, allowing 3-dimensional images of vertical wear and volumetric loss of enamel antagonists to be calculated. One-way analysis of variance followed by Student–Newman–Keuls post hoc tests were used to examine the differences in vertical wear/volumetric loss amongst the groups. Paired t tests and intra-class correlations were used to compare vertical wear/volumetric loss between the micro-CT and intra-oral scanner groups. Results: No significant difference in vertical wear was found amongst all groups. More volumetric loss was found in all test groups than in the control group (P < .001), but no significant difference was found amongst the test groups. There was a moderate positive correlation (r = 0.535, P = .033) between the vertical wear and volumetric loss. No significant difference between the 2 methods of measurement was found. Conclusions: Monolithic ceramics induce more enamel wear than natural teeth. Both micro-CT and intra-oral scanners can be used for measuring tooth wear with similar performance

Candida albicans SAP9: a gene with novel function in hyphal development in Candida albicans

Objectives: To investigate the role of C. albicans Sap9 in yeast-to-hyphal morphological transition. Methods: In this study, SAP9 null mutant strain (Dsap9::hisG/Dsap9::hisG-URA3-hisG) was created by URA blaster method. Ura- phenotype strain was selected in synthetic complete glucose medium containing 0.1% 5-fluoroorotic acid, and complemented by URA3. Hyphal development of fungal cells (1×106 cfu/ml) was evaluated in both liquid and solid hyphal-inducing media, including Lee’s medium (pH 7.0), RPMI-1640, YPD+10% fetal bovine serum (FBS) and Spider’s medium at 37℃. To analyze the mRNA expression of hyphal-specific genes, fungal cells were prepared in the YPD containing 10% FBS at 37 ℃. Total RNA was extracted by SV Total RNA Isolation System and was reverse transcribed into cDNA using Superscirpt II. Gene expression was determined by Fast SYBR Green Master Mix, with the program: 95 ℃ incubation for 20s, and then 40 cycles of 95 ℃ for 1s and 60 ℃ for 20s. Student’s t test (P value \u3c 0.05) was used for statistical analysis. Results: Compared with wild type SC5314 strain, SAP9 null mutant strain was defective in the hyphal development in both liquid and solid hyphal-inducting media. Gene expression analysis indicated that the expression level of transcription factor gene EFG1 was down-regulated by about 40% (P Conclusions: C. albicans SAP9 might play a pivotal role in hyphal development in C. albicans via the cellular events mediated by EFG1

Potential role of Candida albicans secreted aspartic protease 9 in serum induced-hyphal formation and interaction with oral epithelial cells

© 2019 Elsevier Ltd Introduction: Candida albicans possesses the ability to switch rapidly between yeast to hyphal forms. Hyphal formation is a remarkable pathogenic characteristic, which allows C. albicans to invade into host cells. Objectives: This study was to investigate the role of the C. albicans SAP9 gene in hyphal formation and invasion ability. Methods: The morphology of fungal cells in the hyphal-inducing liquid media (YPD+10% fetal bovine serum) was observed by the microscopy. And the morphology of the colony on solid agar plates of YPD+10% fetal bovine serum was photographed by the digital camera. The mRNA expressions of hypha-associated genes in serum medium were also analyzed by real time PCR. Then for the interaction between C. albicans and oral epithelial cells, endocytosis essay, invasion essay and damage assay were performed to compare the differences between the sap9Δ/Δ mutant strain and wild type strain. Results: Compared with the wild type strain, the sap9Δ/Δ mutant strain exhibited a deficient yeast-to-hyphal morphological transition under serum hyphal-inducing conditions. Furthermore, the SAP9 knockout strain revealed a significant down-regulation of the expression of EFG1 (~40%), which is a transcription factor gene that mediates hyphae formation in C. albicans. Compared with the wild type strain, a 70% reduction in the endocytosis of the sap9Δ/Δ mutant strain by host cells was observed, as well as a 25% attenuation of active penetration and a 40% attenuation of host cell damage (P \u3c 0.05). Conclusions: Our data strongly suggests that C. albicans Sap9 is a potential hyphal-associated factor that responds to serum hyphal-inducing stimuli via a cAMP-protein kinase A pathway mediated by EFG1, and contributes to the process of invasion of Candida into the epithelial cells, leading to host cell damage

Effect of Fluoride-Modified Titanium Surface on Early Adhesion of Irradiated Osteoblasts

Objective. The present study aimed to investigate the effect of fluoride-modified titanium surface on adhesion of irradiated osteoblasts. Materials and Methods. Fluoride-modified surface was obtained and the morphology, roughness, and chemical composition of the surface were evaluated by scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy, respectively. The adhesion of irradiated osteoblast-like cells, in terms of number, area, and fluorescence intensity on the titanium surface, was evaluated using immunofluorescence staining. Results. Numerous nanosize pits were seen only in the F-TiO surface. The pits were more remarkable and uniform on F-TiO surface than on TiO surface; however, the amplitude of peaks and bottoms on F-TiO surface appeared to be smaller than on TiO surface. The Sa value and Sdr percentage of TiO surfaces were significantly higher than those of F-TiO surface. The concentrations of main elements such as titanium, oxygen, and carbon were similar on both surfaces. The number of irradiated osteoblasts adhered on the control surface was larger than on fluoride-modified surface. Meanwhile, the cells on the fluoride-modified surface formed more actin filaments. Conclusions. The fluoride-modified titanium surface alters the adhesion of irradiated osteoblasts. Further studies are needed to investigate the proliferation, differentiation, maturation, gene expression, and cytokine production of irradiated osteoblasts on fluoride-modified titanium surface

Effects of Calcium Phosphate Nanocrystals on Osseointegration of Titanium Implant in Irradiated Bone

Radiotherapy may compromise the integration of implant and cause implant loss. Implant surface modifications have the possibility of promoting cell attachment, cell growth, and bone formation which ultimately enhance the osseointegration process. The present study aimed to investigate the effects of calcium phosphate nanocrystals on implant osseointegration in irradiated bone. Sixteen rabbits were randomly assigned into control and nano-CaP groups, receiving implants with dual acid-etched surface or dual acid-etched surface discretely deposited of nanoscale calcium-phosphate crystals, respectively. The left leg of all the rabbits received 15 Gy radiation, followed by implants placement one week after. Four animals in each group were sacrificed after 4 and 12 weeks, respectively. Implant stability quotient (ISQ), ratio of bone volume to total volume (BV/TV), bone growth rate, and bone-to-implant contact (BIC) were evaluated. The nano-CaP group showed significantly higher ISQ (week 12, P=0.031) and bone growth rate (week 6, P=0.021; week 9, P=0.001) than that in control group. No significant differences in BV/TV and BIC were found between two groups. Titanium implant surface modified with CaP nanocrystals provides a potential alternative to improve bone healing around implant in irradiated bone

Antimicrobial Efficacy of Fruit Peels Eco-Enzyme against Enterococcus faecalis: An In Vitro Study

Sodium hypochlorite (NaOCl), an effective endodontic irrigant against Enterococcus faecalis (EF), is harmful to periapical tissues. Natural pineapple-orange eco-enzymes (M-EE) and papaya eco-enzyme (P-EE) could be potential alternatives. This study aimed to assess the antimicrobial efficacy of M-EE and P-EE at different concentrations and fermentation periods against EF, compared to 2.5% NaOCl. Fermented M-EE and P-EE (3 and 6 months) at various concentrations were mixed with EF in a 96-well plate incubated for 24 h anaerobically. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of M-EE and P-EE were determined via EF growth observation. EF inhibition was quantitatively measured and compared between different irrigants using the one-way analysis of variance (ANOVA), and different fermentation periods using the independent-samples T-test. M-EE and P-EE showed MIC at 50% and MBC at 100% concentrations. There was no significant difference in antimicrobial effect when comparing M-EE and P-EE at 50% and 100% to 2.5% NaOCl. P-EE at 6 months fermentation exhibited higher EF inhibition compared to 3 months at concentrations of 25% (p = 0.017) and 0.78% (p = 0.009). The antimicrobial properties of M-EE and P-EE, at both 100% and 50% concentrations, are comparable to 2.5% NaOCl. They could therefore be potential alternative endodontic irrigants, but further studies are required