アトピー性皮膚炎におけるフラクタルカインとその受容体であるCX3CR1の発現 : 皮膚への炎症への関与について

取得学位 : 博士（医学）, 学位授与番号 : 医博乙第1593号, 学位授与年月日 : 平成16年9月1日, 学位授与大学 : 金沢大

Ku-band long distance site-diversity (SD) Characteristics using new measuring system

Abstract—This paper deals with the short (10 km) and long dis-tance (300–1400 km) site-diversity (SD) characteristics by using a newly developed measuring system. In the proposed measuring system, six earth stations transmit 14-GHz band QPSK signals, and one measuring earth station receives 12-GHz band signals and processes them to determine SD characteristics. As a result, easy operation and maintenance, low-cost measuring system construc-tion and highly accurate data have been obtained. By comparing those measured results with the SD joint probability approxima-tion equation in ITU-R Rec.P.618-7, a good agreement can be ob-tained. Furthermore, the effect of typhoons on SD characteristics were measured. Index Terms—Rain attenuation, satellite communication system, site-diversity (SD) measurement system, SD characteristics, VSAT system design. I

Expression of fractalkine and its receptor, CX3CR1, in atopic dermatitis: Possible contribution to skin inflammation

Background: Fractalkine (FKN) induces activation and adhesion of leukocytes expressing its receptor, CX3CR1. FKN is released from the cell surface through proteolytic cleavage as soluble FKN (sFKN). Objective: We sought to assess FKN and CX3CR1 expression in the skin, serum sFKN levels, and CX3CR1 expression on blood leukocytes in patients with atopic dermatitis (AD). Methods: FKN and CX3CR1 expression in the skin was examined immunohistochemically. mRNA expression of FKN, thymus and activation-regulated chemokine, and macrophage-derived chemokine in the skin was assessed by means of real-time RT-PCR. Serum sFKN levels were assessed by using ELISA. Blood leukocytes were stained for CX3CR1 by means of flow cytometric analysis. Results: FKN was strongly expressed on endothelial cells in skin lesions of patients with AD and psoriasis but not in normal skin. FKN mRNA levels in AD lesional skin increased to a similar extent to thymus and activation-regulated chemokine and macrophage-derived chemokine mRNA levels. CX3CR1-expressing cells in the affected skin of patients with AD or psoriasis increased compared with those in normal skin. Serum sFKN levels were increased in patients with AD but not in patients with psoriasis relative to levels in healthy control subjects. Serum sFKN levels were associated with the disease severity and decreased with the improvement of skin lesions in patients with AD. CX3CR1+ cell frequencies and CX3CR1 expression levels were decreased in CD8+ T cells, monocytes, and natural killer cells from patients with AD, but this was not observed in patients with psoriasis. Conclusions: These results suggest that through functions in both membrane-bound and soluble forms, FKN plays an important role in the trafficking of CX3CR1+ leukocytes during the inflammation caused by AD

Antiphospholipid antibodies in patients with autoimmune blistering disease

金沢大学医学部附属病院皮膚科Objective: Our purpose was to determine the serum levels and frequency of antiphospholipid antibodies (aPLs) and confirm the clinical importance of these antibodies in patients with autoimmune blistering disease (ABD). Methods: IgG and IgM anticardiolipin antibodies (aCL), IgG anticardiolipin-β2 glycoprotein I complex antibody (aCL/β2GPI), and IgG antiphosphatidylserine-prothrombin complex antibody (aPS/PT) were examined with an enzyme-linked immunosorbent assay in 71 patients with ABD, including pemphigus vulgaris, pemphigus foliaceus, and bullous pemphigoid. Results: The prevalence of IgG aCL, IgM aCL, aCL/β2GPI, and IgG aPS/PT was positive for 22.4%, 9.1%, 9.9%, and 25.4% of the ABD patients, respectively, whereas these antibodies were not detected in any of the normal control subjects. Ten of 20 patients with ABD who were attending our hospital in 2004 tested positive for aPLs, and thromboembolism was detected in 7 of 10 patients with aPLs. Limitations: Follow-up studies, especially with a large patient group, will be needed to clarify the clinical relevance of aPLs in ABD. Conclusion: aPLs are frequently detected in patients with ABD. Careful examination and follow-up for thromboembolism may be necessary in ABD patients with aPLs. © 2007 American Academy of Dermatology, Inc

Elevated Serum BAFF Levels in Patients with Localized Scleroderma in contrast to other organ-specific autoimmune diseases

Serum levels of B-cell activating factor belonging to the tumor necrosis factor family (BAFF), a potent B-cell survival factor, are elevated in patients with systemic autoimmune diseases, such as systemic lupus erythematosus (SLE), rheumatoid arthritis and systemic sclerosis (SSc). The objective of this study was to determine serum BAFF levels and relate the results to the clinical features in patients with organ-specific autoimmune diseases of the skin, such as localized scleroderma and autoimmune bullous diseases. Serum BAFF levels were examined by enzyme-linked immunosorbent assay in 44 patients with localized scleroderma, 20 with pemphigus vulgaris/pemphigus foliaceus, 20 with bullous pemphigoid and 30 healthy controls. Twenty patients with SSc and 20 with SLE were also examined as disease controls. Serum BAFF levels were elevated in localized scleroderma patients compared with healthy controls. Concerning localized scleroderma subgroups, patients with generalized morphea, the severest form of localized scleroderma, had higher serum BAFF levels than linear scleroderma or morphea patients. The BAFF levels of generalized morphea were comparable with those of SSc or SLE. Furthermore, serum BAFF levels correlated positively with antihistone antibody levels and the severity of skin lesion as well as the number of skin lesions. By contrast, serum BAFF levels were not significantly elevated in patients with pemphigus or pemphigoid. These results suggest that BAFF may be contributing to autoimmunity and disease development in localized scleroderma. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard

イラン南部のバム断層において2003年12月26日バム地震によって生じた地表変位

During the December 26th, 2006 Bam earthquake, continuous ruptures with a consistent rightlateral strike-slip of a few centimeters occurred north of Bam. A 3km long strand of ruptures coincides exactly with the trace of the geologic Bam fault. These ruptures were possibly caused by the tectonic slip on the source fault of the 2006 earthquake. The Bam scarp south of the Zehedan highway might have grown during the earthquake. The extension of the area around the scarp indicated by the scarp-parallel fissures may represent the coseismic stretch of the surface. South of the Bam scarp, there was no systematic surface effect. The absence of significant tectonic offset at the surface is concordant with the intermediate magnitude of Mw 6.6 Only a small and deep portion of the Bam fault, or another adjacent blind fault plane was ruptured in 2006. The geologic evidence of the over 50km long Bam fault suggests a large, probably M 7.5 or larger, event in the future, however, there is no historic and geologic data to quantify the risks

Synthesis and evaluation of a dimeric rgd peptide as a preliminary study for radiotheranostics with radiohalogens

金沢大学疾患モデル総合研究センターWe recently developed125I-and211At-labeled monomer RGD peptides using a novel radi-olabeling method. Both labeled peptides showed high accumulation in the tumor and exhibited similar biodistribution, demonstrating their usefulness for radiotheranostics. This study applied the labeling method to a dimer RGD peptide with the aim of gaining higher accumulation in tumor tissues based on improved affinity with αvβ3 integrin. We synthesized an iodine-introduced dimer RGD peptide, E[c(RGDfK)] (6), and an 125/131 I-labeled dimer RGD peptide, E[c(RGDfK)]{[125/131I]c[RGDf(4-I)K]} ([125/131I]6), and evaluated them as a preliminary step to the synthesis of an211At-labeled dimer RGD peptide. The affinity of 6 for αvβ3 integrin was higher than that of a monomer RGD peptide. In the biodistribution experiment at 4 h postinjection, the accumulation of [125I]6 (4.12 ± 0.42% ID/g) in the tumor was significantly increased compared with that of125I-labeled monomer RGD peptide (2.93 ± 0.08% ID/g). Moreover, the accumulation of [125I]6 in the tumor was greatly inhibited by co-injection of an excess RGD peptide. However, a single injection of [131I]6 (11.1 MBq) did not inhibit tumor growth in tumor-bearing mice. We expect that the labeling method for targeted alpha therapy with211At using a dimer RGD peptide could prove useful in future clinical applications. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.CC-BY 4.

High-resolution seismic reflection profiling across the Shiraiwa fault, eastern margin of the Yokote basin fault zone, northeast Japan : data acquisition and processing

The eastern margin of the Yokote basin fault zone extends about 56km at the western foot of the Ou Backbone Range, northeast Japan. The Rikuu earthquake (M=7.2) occurred in the Ou Backbone Range (Mahiru Range) on 31st August, 1896. Associated with this earthquake, four thrust faults-Obonai, Shiraiwa, Ota, and Senya fault3 appeared on the surface of the western foot of the Mahiru Range. These faults were highly sinuous with numerous gaps and en echelon steps. We conducted a high-resolution seismic reflection profiling survey across the Shiraiwa fault. The obtained seismic reflection data were processed by conventional common mid-point methods, post-stack migration, and depth conversion. The subsurface structure across the Shraiwa fault is characterized by branched low-angle reverse faults and conjugate back-thrust. The emergent thrust associated with the 1896 earthquake is regarded to be a subsidiary reverse fault