Projet MANOE, Maîtrise Allergène NutritiOn Enfant. Etude de la réactivité de patients allergiques à des faibles doses d'allergènes (blé, œuf, lait, arachide). Détection et quantification des allergènes présents à faibles doses dans des matrices alimentaires complexes

Ce numéro comprend les articles correspondant aux présentations du Colloque Allergies, intolérances et hypersensibilités alimentaires, qui s’est tenu à Paris le 17 juin 2016.In the absence of validated clinical thresholds and of subsequent regulatory thresholds, allergen risk management is not satisfactory for all stakeholders. For industry a real risk of cross contamination at a low level have to be labelled even though most of the allergic people might tolerate this low dose. On their side, patients faced multiple and inconsistent advised labels on food packaging. The MANOE project addressed this threshold issue to explore: 1/if it is possible to identify allergic children who tolerated low amount of allergen; 2/ how allergic people can be informed about their tolerance to low amount of allergen; 3/if commercial analytical methods are sensitive and quantitative to guaranty a risk of contamination below a threshold. An oral challenge with low doses of peanut, milk, wheat or egg was designed and enabled the identification of patients who tolerated low amount of allergen and it facilitated acceptance of a less restricted diet. Analytical methods detected all contaminations at 10 mg/kg but were not accurate for quantification of egg, milk and peanut notably in processed foods.En l’absence de seuils cliniques reconnus et de seuils réglementaires qui en découleraient, la gestion du risque allergène est insatisfaisante pour tous les acteurs concernés. Pour les industriels un risque avéré de contamination croisée à une dose très faible se doit d’être étiqueté même s’il apparait de plus en plus clairement que la plupart des patients allergiques pourraient consommer cette dose sans y réagir. De leur côté, les patients se trouvent confrontés à de multiples messages d’avertissement sans consistance. Le projet MANOE s’est placé dans un contexte de seuils, cliniques et analytiques, pour voir : 1/ S’il était possible d’identifier les enfants tolérants de petites doses d’allergène ; 2/ Comment cette information de tolérance de faible dose d’allergène pourraient être transmise au patient ; 3/ Si les méthodes analytiques commercialisées permettaient de détecter et de quantifier une contamination à une faible concentration. Appliqué à l’arachide, au blé, au lait et à l’œuf, un test oral de réintroduction a permis d’identifier les patients tolérants de petites doses et de faciliter l’acceptation d’un assouplissement de leur régime alimentaire. Les méthodes analytiques commercialisées se sont montrées capables de détecter des contaminations de 10 mg/kg mais pas de les quantifier dans le cas du lait, de l’arachide et de l’œuf

Cell wall antibody collection

National audienc

La collection d'Anticorps Cepia des outils pour explorer grains et graines

National audienc

Detection and quantification of staphylococcal enterotoxin A in foods with specific and sensitive polyclonal antibodies

International audienceStaphyloccocus aureus is reported to be one of the most frequent pathogens involved in food-borne diseases associated with dairy products, especially with raw milk cheese. Some strains produce enterotoxins as Staphylococcal enterotoxin A (SEA) which is involved in 75% of food poisoning outbreaks. Actually few methods are both sensitive and specific enough for confirming the diagnosis of staphylococcal food poisoning. In this work, an Enzyme-Linked ImmunoSorbent Assay (ELISA) was set up and optimised to detect SEA in milk and cheese. Various anti-SEA antibodies: polyclonal sera, anti-peptide polyclonal sera and monoclonal antibodies, used for the capture and or detection steps were compared and analysed. The mouse anti-whole SEA polyclonal serum, used as detection detected 32 pg/ml SEA in buffer and 64 pg/ml in milk. These concentrations were well under the limit set for food safety. This assay was also highly specific of SEA and no cross-reaction was observed with the other staphylococcal enterotoxins. In contaminated cheese samples the time of enterotoxin extraction was reduced by using ultrafiltration method instead of dialysis and the detection limits were 1.5-2.5 and 1.9-3 times more sensitive than a commercial kit and the official method, respectively. Preliminary study of SEA detection with the piezoelectric immunosensor allowed detecting and quantifying SEA within 10 min in unprocessed food but the sensitivity was not sufficient. The ELISA assay with mouse antibodies is likely suitable for SEA routine detection not only in dairy product but also in various foods as sauce and liver mousse. (C) 2012 Elsevier Ltd. All rights reserved

Caractérisation de la variabilité sérologique du virus Y de la pomme de terre (PVY) : impact sur le diagnostic

Bgpi : équipe 6absen

A biology-driven approach for producing monoclonal antibodies against green algal cell wall components

A biology-driven approach for producing monoclonal antibodies against green algal cell wall components. 14th cell wall meetin

Antibodies Collection dedicated to food components

absen

Antibodies Collection dedicated to food components

absen

Biology-driven, microarray-assisted selection of cell wall directed antibodies

Biology-driven, microarray-assisted selection of cell wall directed antibodies. Gordon Research Conference on Plant Cell Wall