Narrowing the communication gap in internationally distributed teams: the case of software-development teams in Sri Lanka and Japan

Communication between geographically separated subgroups in internationally distributed teams (IDTs) is quite challenging because their communication is relatively sparse and relies heavily on electronic media. In the current study, we employed a grounded theory approach and conducted an in-depth case study of two IDTs with subgroups in Sri Lanka and Japan to investigate why communication problems occur between the subgroups and how these can be solved. The findings indicated that although language fluency did not pose a serious threat, the teams encountered communication problems because they did not develop a well-shared team mental model (TMM). Our study further revealed that project process models (PPMs) play a key role in developing well-shared TMMs in IDTs, and the underlying process is facilitated by bridge individuals. Our findings extend the knowledge-sharing perspective of IDTs by focusing on the role of PPM, TMM, and bridge individuals in the communication process in IDTs

How Inpatriates Internalize Corporate Values at Headquarters: The Role of Developmental Job Assignments and Psychosocial Mentoring

Multinational companies (MNCs) often invite foreign subsidiary employees or inpatriates to their headquarters (HQ) to internalize the MNCs’ corporate values and transfer those values to their subsidiaries after repatriation. However, there is a lack of understanding about how and why inpatriates internalize these corporate values during their HQ experiences. By integrating the perspectives of international adjustment and organizational socialization with that of on-the-job learning, we develop a model wherein the job-related and psychosocial factors that inpatriates encounter at HQ promote their internalization of corporate values. Using a sample of 110 foreign subsidiary employee–supervisor dyads from the HQ of a Japanese MNC to which the employees were assigned as inpatriates, we found that developmental job assignments and psychosocial mentoring during inpatriation influenced the internalization of corporate values, which was partially and sequentially mediated by proactive socialization behavior and organizational identification. This study’s findings have significant implications for the theory and practice of inpatriation management, particularly with regard to how MNCs promote the internalization of corporate values among inpatriates

Continuous ERK Activation Downregulates Antiproliferative Genes throughout G1 Phase to Allow Cell-Cycle Progression

SummaryBackgroundThe ERK family of MAP kinase plays a critical role in growth factor-stimulated cell-cycle progression from G0/G1 to S phase. It has been suggested that sustained activation, but not transient activation, of ERK is necessary for inducing S phase entry. Although the essential role of ERK MAP kinase in growth factor-stimulated gene expression, especially expression of immediate-early genes, is well established, it has remained unclear how ERK activity duration affects the promotion of G1 phase progression to S phase.ResultsWe have found that inhibition of ERK activation by the MEK inhibitor or dominant-negative MEK1 even immediately before the onset of S phase leads to the cessation of S phase entry. Our analyses reveal that there are ERK-dependent downregulated genes, whose expression levels return to their original levels rapidly after ERK inactivation, and that their downregulation mostly requires AP-1 activity. Remarkably, microinjection experiments demonstrate that many of the downregulated genes act as antiproliferative genes during G1 phase and that their forced expression to the levels before growth factor stimulation even in late G1 phase blocks S phase entry.ConclusionsThus, continuous ERK activation downregulates antiproliferative genes until the onset of S phase to allow successful G1 phase progression. This mechanism may also work as a fail-safe mechanism, which prevents inappropriate stimuli that induce transient ERK activation from causing S phase entry

Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues

© The Author(s) 2022The emerging field of synthetic developmental biology proposes bottom-up approaches to examine the contribution of each cellular process to complex morphogenesis. However, the shortage of tools to manipulate three-dimensional (3D) shapes of mammalian tissues hinders the progress of the field. Here we report the development of OptoShroom3, an optogenetic tool that achieves fast spatiotemporal control of apical constriction in mammalian epithelia. Activation of OptoShroom3 through illumination in an epithelial Madin-Darby Canine Kidney (MDCK) cell sheet reduces the apical surface of the stimulated cells and causes displacements in the adjacent regions. Light-induced apical constriction provokes the folding of epithelial cell colonies on soft gels. Its application to murine and human neural organoids leads to thickening of neuroepithelia, apical lumen reduction in optic vesicles, and flattening in neuroectodermal tissues. These results show that spatiotemporal control of apical constriction can trigger several types of 3D deformation depending on the initial tissue context.his work was supported by internal grants from RIKEN and EMBL; Grant-in-Aid for Scientific research (KAKENHI) programs from Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT) (16H06170 and 18H04769 to M.E.); Research foundation for OptoScience and Technology (to M.E.). X.T. is funded by European Research Council (Adv-883739) and La Caixa Foundation (LCF/PR/HR20/52400004). IBEC is a recipient of a Severo Ochoa Award of Excellence from the MINECO. M.B.-P. is funded by MICINN (FPI grant, PRE2019-088998)Peer ReviewedArticle signat per 10 autors/es: Guillermo Martínez-Ara, Núria Taberner, Mami Takayama, Elissavet Sandaltzopoulou, Casandra E. Villava, Miquel Bosch-Padrós, Nozomu Takata, Xavier Trepat, Mototsugu Eiraku and Miki Ebisuya.Postprint (published version

Small RNAs Establish Delays and Temporal Thresholds in Gene Expression

Non-coding RNAs are crucial regulators of gene expression in prokaryotes and eukaryotes, but it remains poorly understood how they affect the dynamics of transcriptional networks. We analyzed the temporal characteristics of the cyanobacterial iron stress response by mathematical modeling and quantitative experimental analyses, and focused on the role of a recently discovered small non-coding RNA, IsrR. We found that IsrR is responsible for a pronounced delay in the accumulation of isiA mRNA encoding the late-phase stress protein, IsiA, and that it ensures a rapid decline in isiA levels once external stress triggers are removed. These kinetic properties allow the system to selectively respond to sustained (as opposed to transient) stimuli, and thus establish a temporal threshold, which prevents energetically costly IsiA accumulation under short-term stress conditions. Biological information is frequently encoded in the quantitative aspects of intracellular signals (e.g., amplitude and duration). Our simulations reveal that competitive inhibition and regulated degradation allow intracellular regulatory networks to efficiently discriminate between transient and sustained inputs

Integration of a Phosphatase Cascade with the MAP Kinase Pathway provides for a Novel Signal Processing Function

We mathematically modeled the receptor-activated MAP kinase signaling by incorporating the regulation through cellular phosphatases. Activation induced the alignment of a phosphatase cascade in parallel with the MAP kinase pathway. A novel regulatory motif was thus generated, providing for the combinatorial control of each MAPK intermediate. This ensured a non-linear mode of signal transmission with the output being shaped by the balance between the strength of input signal, and the activity gradient along the phosphatase axis. Shifts in this balance yielded modulations in topology of the motif, thereby expanding the repertoire of output responses. Thus we identify an added dimension to signal processing, wherein the output response to an external stimulus is additionally filtered through indicators that define the phenotypic status of the cell.Comment: Whole Manuscript 33 pages inclduing Main text, 7 Figures and Supporting Informatio

Characterization of Transcription Start Sites of Putative Non-coding RNAs by Multifaceted Use of Massively Paralleled Sequencer

On the basis of integrated transcriptome analysis, we show that not all transcriptional start site clusters (TSCs) in the intergenic regions (iTSCs) have the same properties; thus, it is possible to discriminate the iTSCs that are likely to have biological relevance from the other noise-level iTSCs. We used a total of 251 933 381 short-read sequence tags generated from various types of transcriptome analyses in order to characterize 6039 iTSCs, which have significant expression levels. We analyzed and found that 23% of these iTSCs were located in the proximal regions of the RefSeq genes. These RefSeq-linked iTSCs showed similar expression patterns with the neighboring RefSeq genes, had widely fluctuating transcription start sites and lacked ordered nucleosome positioning. These iTSCs seemed not to form independent transcriptional units, simply representing the by-products of the neighboring RefSeq genes, in spite of their significant expression levels. Similar features were also observed for the TSCs located in the antisense regions of the RefSeq genes. Furthermore, for the remaining iTSCs that were not associated with any RefSeq genes, we demonstrate that integrative interpretation of the transcriptome data provides essential information to specify their biological functions in the hypoxic responses of the cells

The identification of trans-associations between prostate cancer GWAS SNPs and RNA expression differences in tumor-adjacent stroma

Here we tested the hypothesis that SNPs associated with prostate cancer risk, might differentially affect RNA expression in prostate cancer stroma. The most significant 35 SNP loci were selected from Genome Wide Association (GWA) studies of ~40,000 patients. We also selected 4030 transcripts previously associated with prostate cancer diagnosis and prognosis. eQTL analysis was carried out by a modified BAYES method to analyze the associations between the risk variants and expressed transcripts jointly in a single model. We observed 47 significant associations between eight risk variants and the expression patterns of 46 genes. This is the first study to identify associations between multiple SNPs and multiple in trans gene expression differences in cancer stroma. Potentially, a combination of SNPs and associated expression differences in prostate stroma may increase the power of risk assessment for individuals, and for cancer progression