52 research outputs found
Preparation and Dynamic Patterning of Supported Lipid Membranes Mimicking Cell Membranes
A Frame-Shift Mutation in the SLC34A2 Gene in Three Patients with Pulmonary Alveolar Microlithiasis in an Inbred Family
Selective Estrogen Receptor Modulator Raloxifene-Associated Aggravation of Nonalcoholic Steatohepatitis
Evaluation of Nanoporous Gold with Controlled Surface Structures for Laser Desorption Ionization (LDI) Analysis: Surface Area Versus LDI Signal Intensity
Bilayer lipid membranes from falling droplets
We describe a system that provides a rapid and simple way of forming suspended lipid bilayers within a microfluidic platform from an aqueous droplet. Bilayer lipid membranes are created in a polymeric device by contacting monolayers formed at a two-phase liquid-liquid interface. Microdroplets, containing membrane proteins, are injected onto an electrode positioned above an aperture machined through a conical cavity that is filled with a lipid-alkane solution. The formation of the BLM depends solely on the device geometry and leads to spontaneous formation of lipid bilayers simply by dispensing droplets of buffer. When an aqueous droplet containing transmembrane proteins or proteoliposomes is injected, straightforward electrophysiology measurements are possible. This method is suitable for incorporation into lab-on-a-chip devices and allows for buffer exchange and electrical measurements
Protons are One of the Limiting Factors in Determining Sensitivity of Nano Surface-Assisted (+)-Mode LDI MS Analyses
Surfactant-assisted fabrication of free-standing inorganic sheets covering an array of micrometre-sized holes
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