Reconfigurable 1.5D Source Arrays for Improved Elevational Focussing in All-Optical Ultrasound Imaging

Recently, an all-optical ultrasound imaging system was presented that was capable of real-time, video-rate 2D pulse-echo imaging. This was achieved through the use of rapid scanning optics and a centimetre-scale optical ultrasound generator, which allowed for the synthesis of 1D source arrays of arbitrary geometry. Back-scattered ultrasound was recorded using a highly sensitive fibre-optic ultrasound detector. Here, a modification to this system is presented that enabled the synthesis of arbitrary 2D source array geometries. This extension allowed for the implementation of 1.5D arrays, which we demonstrated to yield improved elevational resolution (1.1 mm for 1D arrays versus 0.9 mm for 1.5D arrays)at the expense of a decrease (-3.4 dB)in signal-to-clutter level. The axial (115 μm)and lateral (320 μm)resolutions, as well as the acquisition time, remained unchanged, as was shown using phantoms and ex vivo tissue samples. In addition, the 1.5D source array geometry enabled 3D out-of-plane tracking (0.7 mm resolution across a range of ±1.5 mm)while performing high-quality 2D imaging. The presented ability to synthesise arbitrary, two-dimensional source array geometries will greatly improve and facilitate ultrasound imaging and guidance of interventional procedures

Correlation of Ultrasound Shear Wave Elastography with Pathological Analysis in a Xenografic Tumour Model.

The objective of this study was to evaluate the potential value of ultrasound (US) shear wave elastography (SWE) in assessing the relative change in elastic modulus in colorectal adenocarcinoma xenograft models in vivo and investigate any correlation with histological analysis. We sought to test whether non-invasive evaluation of tissue stiffness is indicative of pathological tumour changes and can be used to monitor therapeutic efficacy. US-SWE was performed in tumour xenografts in 15 NCr nude immunodeficient mice, which were treated with either the cytotoxic drug, Irinotecan, or saline as control. Ten tumours were imaged 48 hours post-treatment and five tumours were imaged for up to five times after treatment. All tumours were harvested for histological analysis and comparison with elasticity measurements. Elastic (Young's) modulus prior to treatment was correlated with tumour volume (r = 0.37, p = 0.008). Irinotecan administration caused significant delay in the tumour growth (p = 0.02) when compared to control, but no significant difference in elastic modulus was detected. Histological analysis revealed a significant correlation between tumour necrosis and elastic modulus (r = -0.73, p = 0.026). SWE measurement provided complimentary information to other imaging modalities and could indicate potential changes in the mechanical properties of tumours, which in turn could be related to the stages of tumour development

Ultra-Fast and Sensitive Detection of Non-Typhoidal Salmonella Using Microwave-Accelerated Metal-Enhanced Fluorescence (“MAMEF”)

Certain serovars of Salmonella enterica subsp. enterica cause invasive disease (e.g., enteric fever, bacteremia, septicemia, meningitis, etc.) in humans and constitute a global public health problem. A rapid, sensitive diagnostic test is needed to allow prompt initiation of therapy in individual patients and for measuring disease burden at the population level. An innovative and promising new rapid diagnostic technique is microwave-accelerated metal-enhanced fluorescence (MAMEF). We have adapted this assay platform to detect the chromosomal oriC locus common to all Salmonella enterica subsp. enterica serovars. We have shown efficient lysis of biologically relevant concentrations of Salmonella spp. suspended in bacteriological media using microwave-induced lysis. Following lysis and DNA release, as little as 1 CFU of Salmonella in 1 ml of medium can be detected in <30 seconds. Furthermore the assay is sensitive and specific: it can detect oriC from Salmonella serovars Typhi, Paratyphi A, Paratyphi B, Paratyphi C, Typhimurium, Enteritidis and Choleraesuis but does not detect Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae or Acinetobacter baumanii. We have also performed preliminary experiments using a synthetic Salmonella oriC oligonucleotide suspended in whole human blood and observed rapid detection when the sample was diluted 1∶1 with PBS. These pre-clinical data encourage progress to the next step to detect Salmonella in blood (and other ordinarily sterile, clinically relevant body fluids)

Operational precise irrigation for cotton cultivation through the coupling of meteorological and crop growth models.

In this paper, we tested the operational capacity of an interoperable model coupling system for the irrigation scheduling (IMCIS) at an experimental cotton (Gossypium hirsutum L.) field in Northern Greece. IMCIS comprises a meteorological model (TAPM), downscaled at field level, and a water-driven cultivation tool (AquaCrop), to optimize irrigation and enhance crop growth and yield. Both models were evaluated through on-site observations of meteorological variables, soil moisture levels and canopy cover progress. Based on irrigation management (deficit, precise and farmer’s practice) and method (drip and sprinkler), the field was divided into six sub-plots. Prognostic meteorological model results exhibited satisfactory agreement in most parameters affecting ETo, simulating adequately the soil water balance. Precipitation events were fairly predicted, although rainfall depths needed further adjustment. Soil water content levels computed by the crop growth model followed the trend of soil humidity measurements, while the canopy cover patterns and the seed cotton yield were well predicted, especially at the drip irrigated plots. Overall, the system exhibited robustness and good predicting ability for crop water needs, based on local evapotranspiration forecasts and crop phenological stages. The comparison of yield and irrigation levels at all sub-plots revealed that drip irrigation under IMCIS guidance could achieve the same yield levels as traditional farmer’s practice, utilizing approximately 32% less water, thus raising water productivity up to 0.96 kg/m3.N/