In vivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum(Wïlld.) Muell. Arg.

ObjectiveTo evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum (B. montanum) (Willd.) Muell.-Arg. leaves and root.MethodsThe in vitro derived rootlets and leaves segments of B. montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3% sucrose, gelled with 0.7% agar and different concentration of 2, 4-D either alone or in combinations. The preliminary phytochemical screening was performed by Harborne method. Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37°C.ResultsThe highest percentage of callus formation (leaves segments 86.9±0.56; root segments 78.7±0.51) was obtained on Murashige and Skoog's basal medium supplemented with 3% sucrose and 2.0 mg/L of 2,4-Dichlorophenoxy acetic acid. The phytochemical study revealed the high quantity presence of steroids, triterpenoids, glycosides, saponins, alkaloids, flavanoids, phenolic compounds, tannins, sugars etc of root and leaves derived calli. The ethanol extract of leaves segment derived calli of B. montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200 μL.ConclusionThe preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots. The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds

Expression of LIM kinase 1 is associated with reversible G1/S phase arrest, chromosomal instability and prostate cancer

<p>Abstract</p> <p>Background</p> <p>LIM kinase 1 (LIMK1), a LIM domain containing serine/threonine kinase, modulates actin dynamics through inactivation of the actin depolymerizing protein cofilin. Recent studies have indicated an important role of LIMK1 in growth and invasion of prostate and breast cancer cells; however, the molecular mechanism whereby LIMK1 induces tumor progression is unknown. In this study, we investigated the effects of ectopic expression of LIMK1 on cellular morphology, cell cycle progression and expression profile of LIMK1 in prostate tumors.</p> <p>Results</p> <p>Ectopic expression of LIMK1 in benign prostatic hyperplasia cells (BPH), which naturally express low levels of LIMK1, resulted in appearance of abnormal mitotic spindles, multiple centrosomes and smaller chromosomal masses. Furthermore, a transient G1/S phase arrest and delayed G2/M progression was observed in BPH cells expressing LIMK1. When treated with chemotherapeutic agent Taxol, no metaphase arrest was noted in these cells. We have also noted increased nuclear staining of LIMK1 in tumors with higher Gleason Scores and incidence of metastasis.</p> <p>Conclusion</p> <p>Our results show that increased expression of LIMK1 results in chromosomal abnormalities, aberrant cell cycle progression and alteration of normal cellular response to microtubule stabilizing agent Taxol; and that LIMK1 expression may be associated with cancerous phenotype of the prostate.</p