5 research outputs found
The CphAII protein from Aquifex aeolicus exhibits a metal-dependent phosphodiesterase activity
- Author
- A Carfi
- A Vogel
- AD Cameron
- AF Neuwald
- AF Yakunin
- C Bebrone
- C Bebrone
- C Bebrone
- C Bebrone
- C Frazao
- Carine Bebrone
- CĂ©dric Bauvois
- D Liu
- DJ Miller
- E Kuznetsova
- EG Orellano
- G Deckert
- G Garau
- G Garau
- G Hagelueken
- H Daiyasu
- I Callebaut
- JD Bendtsen
- Jean-Marie Frère
- K Podzelinska
- Kurt Hoffmann
- L Aravind
- L Whitmore
- M Hernandez-Valladares
- M Hernandez-Valladares
- M Vanhove
- M Wenzel
- Michaël Kupper
- Moreno Galleni
- O Jacquin
- O Schilling
- R Huber
- R Ishii
- R Sapranauskas
- RL Kelley
- S Chen
- T Kawasumi
- TA Murphy
- Y Zhao
- YJ Dong
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2012
- Field of study
The CphAII protein from the hyperthermophile Aquifex aeolicus shows the five conserved motifs of the metallo-β-lactamase (MBL) superfamily and presents 28% identity with the Aeromonas hydrophila subclass B2 CphA MBL. The gene encoding CphAII was amplified by PCR from the A. aeolicus genomic DNA and overexpressed in Escherichia coli using a pLex-based expression system. The recombinant CphAII protein was purified by a combination of heating (to denature E. coli proteins) and two steps of immobilized metal affinity chromatography. The purified enzyme preparation did not exhibit a β-lactamase activity but showed a metal-dependent phosphodiesterase activity versus bis-p-nitrophenyl phosphate and thymidine 5'-monophosphate p-nitrophenyl ester, with an optimum at 85°C. The circular dichroism spectrum was in agreement with the percentage of secondary structures characteristic of the MBL αββα fold
An integrated biophysical approach to discovering mechanisms of NDM-1 inhibition for several thiol-containing drugs
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Metallo-β-lactamases withstand low Zn(II) conditions by tuning metal-ligand interactions
- Author
- A Badarau
- A Carfi
- Alejandro J Vila
- AV Davis
- CE Outten
- EG Orellano
- F Simona
- Francisco J Medrano MartĂn
- H Daiyasu
- J Morán-Barrio
- Javier M González
- JF Fisher
- JM González
- Julia A Cricco
- LI Llarrull
- LI Llarrull
- MarĂa-RocĂo Meini
- MF Tioni
- MJ Hawk
- MW Crowder
- O Jacquin
- Pablo E Tomatis
- RM Rasia
- S Haruta
- S Wommer
- SM Fabiane
- TA Murphy
- Z Hu
- Z Ma
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Plant probiotic bacteria: solutions to feed the world
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- Spaepen S Vanderleyden J, Remans R
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- Trewavas A
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- Verma VC Singh SK, Prakash S
- Vessey JK Pawlowski K, Bergman B
- Wang W Vinocur B, Altman A
- Wani SA Chand S, Ali T
- Xu S Kim BS
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- Zhang C Kong F
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- Publication venue
- 'American Institute of Mathematical Sciences (AIMS)'
- Publication date
- 01/01/2017
- Field of study
The roles of calcium and phosphoinositides in the mechanisms of α 1-adrenergic and other agonists
- Author
- A Binet
- A Delvaux
- A DenHertog
- A Enjalbert
- A Fein
- A Imai
- A Jakob
- A Kikuchi
- A Kishimoto
- A Spat
- A Spat
- A Wolfman
- A Yatani
- AA Abdel-Letif
- AC Nairn
- AC Nairn
- AH Merrill Jr
- AH Tashjian Jr
- AJ Baukal
- AK Ho
- AK Ho
- AL Morrow
- AM Capponi
- AP Dawson
- AP Dawson
- AP Doskeland
- AP Somlyo
- AP Tarver
- AP Thomas
- AP Thomas
- AR Hughes
- AR Means
- AS Kraft
- AS Kraft
- AV Somlyo
- B Baudiere
- B Berthon
- B Berthon
- B Bouscarel
- B Bouscarel
- B Breant
- B Ciapa
- BA Davis
- BA Wolf
- BA Wolf
- BB Hoffman
- BB Wolfe
- BC Tilly
- BD Gomperts
- BE Burke
- BE Miller
- BE Slack
- BF Dickey
- BI Terman
- BJ Holub
- BP Hughes
- BP Hughes
- BP Hughes
- BR Ganong
- BW Agranoff
- C Bruns
- C Cochet
- C Dop Van
- C Erneux
- C Erneux
- C Han
- C Picton
- CA Hansen
- CA Harrington
- CA Vandenberg
- CB Klee
- CC Malbon
- CC Malbon
- CC Malbon
- CC Malbon
- CD Benham
- CD Smith
- CD Smith
- CE King
- CE Seidman
- CF Bennett
- CF Strnad
- CH MacPhee
- CJ Kirk
- CJ Kirk
- CJ LePeuch
- CJ Lynch
- CJ Lynch
- CJ Lynch
- CJ Lynch
- CJ Lynch
- CJ Lynch
- CJ Lynch
- CK Pushpendran
- CL Brooks
- CM Schworer
- CM Schworer
- CO Rock
- CP Downes
- CP Downes
- CP Downes
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- CS Chew
- CW Taylor
- CW Taylor
- D Allan
- D Bojanic
- D Buxton
- D Corda
- D Delbeke
- D Haussinger
- D Nicholls
- D Renard
- D Rhodes
- D Schlondorff
- D Sugden
- DA Bass
- DA Hems
- DB Bylund
- DB Wilson
- DB Wilson
- DE Logothetis
- DE MacIntyre
- DF Babcock
- DG Haylett
- DG Nicholls
- DH Jenkinson
- DH Walker
- DH Williamson
- DJ Hall
- DJ Jones
- DJ Storey
- DJ Stumpo
- DK Ways
- DL Alkon
- DL Aub
- DL Aub
- DL Aub
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- DL Lewis
- DM Delfert
- DM Waisman
- DO Lucas
- DR Manning
- DS Drust
- DT Yamaguchi
- E Carafoli
- E Murphy
- E Nadler
- E Nanberg
- E Suematsu
- E Wilson
- EB Hollingsworth
- EE Reynolds
- EG Lapetina
- EJ Geras
- EJ Nestler
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- ER Trimble
- EZ Szuts
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- H Deckmyn
- H Higashida
- H Ishii
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- H Kamagai
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- H Reuter
- H Schulman
- H Schulman
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- H Schulman
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- H Storm
- H Streb
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- HM Korchak
- HR Carter
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- I Crossley
- I Fuse
- I Kojima
- I Litosch
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- JB Imboden
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- JB Smith
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- JM Baraban
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- MA Seyfred
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- MC Cabot
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- MF El-Refai
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- MF Rossier
- MG Low
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- MI Gonzatti-Haces
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- MR Hokin
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- MW Banschbach
- MW Martin
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- P Thomopoulos
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- PA Connelly
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- PC Sternweis
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- PD Lew
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- PJ Blackshear
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- RD Brown
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- RI Sha'afi
- RJ Davis
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- RJ Haslam
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- RJ Parod
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- RJ Schimmel
- RJ Uhing
- RJ Uhing
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- RK Studer
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- RK Studer
- RM Burch
- RM Burch
- RM Denton
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- RM Graham
- RM Graham
- RMC Dawson
- RN Kolesnick
- RO Morgan
- RP Ebstein
- RP Rubin
- RS Adelstein
- RS Adelstein
- RS Balaban
- RW Dougherty
- RY Tsien
- RY Tsien
- RY Tsien
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- S Chacko
- S Cockcroft
- S Cockcroft
- S Cockeroft
- S Corvera
- S Corvera
- S Corvera
- S Jackowski
- S Jacobs
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- S Muallem
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- S Orellano
- S Paris
- S Rittenhouse-Simmons
- S Shenolikar
- S Shenolikar
- S Yamada
- S-H Chueh
- S-H Chueh
- SA Berkowitz
- SA DeRiemer
- SA DeRiemer
- SA Orellana
- SB Bocckino
- SB Bocckino
- SB Dillon
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- SB Masters
- SB Shears
- SB Shears
- SB Shears
- SB Shears
- SD Shukla
- SE Rittenhouse
- SE Rittenhouse
- SF Steinberg
- SH Lin
- SH Lin
- SH Marier
- SH Ryu
- SH Ryu
- SJ Brandt
- SJ Pandol
- SJ Stewart
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- SJ Stoehr
- SJ Taylor
- SJ Weiss
- SJ Weiss
- SK Ambler
- SK Joseph
- SK Joseph
- SK Joseph
- SK Joseph
- SK Joseph
- SK Joseph
- SK Joseph
- SL Hoffman
- SL Pelech
- SL Pocotte
- SL Pocotte
- SM Shreeve
- SMT Hernandez-Sotomayor
- SO Moon
- SO Sage
- SP Driska
- SR Coughlin
- SR Slivka
- SZ Langer
- SZ Langer
- T Balla
- T Balla
- T Evans
- T Evans
- T Jean
- T Katada
- T Katada
- T Katada
- T Matozaki
- T Michel
- T Mine
- T Mori
- T Murayama
- T Nabika
- T Nakamura
- T Nakamura
- T Nukada
- T Pozzan
- T Pozzan
- T Sasaguri
- T Sasaki
- T Sugano
- T Uchida
- T Ueda
- T Yamauchi
- T Yamauchi
- TB Bolton
- TFJ Martin
- TFJ Martin
- TFJ Martin
- TJ Biden
- TJ Biden
- TJ Biden
- TJ Biden
- TJ Fitzgerald
- TJ Rink
- TJ Rink
- TK Harden
- TL Lambert
- TL McGuinness
- TL McGuinness
- TM Chan
- TM Chan
- TM Chan
- TM Connolly
- TM Connolly
- TM Connolly
- TM Connolly
- TM Nosek
- TP Stossel
- TR Hesketh
- U Kikkawa
- U Kikkawa
- V Chiarugi
- V Henne
- V Henne
- V Manne
- V Niggli
- V Prpic
- V Prpic
- VS Bansal
- VS Pribluda
- W Kruijer
- W Rosenthal
- W Schmitz
- W Zawalich
- WB Busa
- WG Strickland
- WG Strickland
- WJ Malaisse
- WK Pollock
- WM Taylor
- WM Taylor
- WS Colucci
- WS Colucci
- WS May
- WS May Jr
- WWY Lo
- WWY Lo
- WY Cheung
- Y Banno
- Y Banno
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- Y Kanaho
- Y Katakami
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- Y Ono
- Y Ono
- Y Oron
- Y Takai
- Y Takai
- Y-T Xuan
- YA Hannun
- YA Hannun
- YA Hannun
- YA Hannun
- YA Hannun
- Z Ahmad
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/1988
- Field of study