A Collection of the Biographies of Dr. Hideyo Noguchi in Matsumoto Dental University (The last Report)

Previously I presented 291 publications regarding biographies of Dr. Hideyo Noguchi and his related persons to Matsumoto Shigaku (13 : 1–34, 198₇ ; 15 : 21₇–231, 198₇ ; 20 : 80–99, 1994 ; 23 : 194–210, 199₇ ; 26 : 13₇–145, 2000). In this paper an additional book (Fig. 242), a color silver coin (Fig. 243), 4 postage stamps (Figs. 244–24₇) and a medal (2008, Figs. 248, A, B) were described. Noguchiʼs postage stamps were issued already 2 (1949 and 2013) in Japan ; 2 (19₇6) in Ecuador; 1 (1993) in Guyana; 3 (199₇) in Ghana. The Noguchiʼs memorial coin is the first in the medical world of Japan

形態計測のためのメタノール・硝酸セルロースによる組織包埋法

Very recently we developed a new embedding medium, "Shiojirin-E", made chiefly of cellulose nitrate (Kawakami et al. 1995)^&;t;1\u272)>. During the course of its development, we noted that cellulose nitrate would be a very suitable embedding medium for morphometric analysis, especially because of its low and constant rate of contraction. But the highviscosity of this medium resulted in a very slow rate of infiltration. Therefore we sought to develop a new, better histological embedding medium for morphometric analysis based on information on the methanol-cellulose nitrate method by Seki (1937)^. After trial and error, we succeeded in our goal : The composition of the medium is as follows : cellulose nitrate 15.0g, methanol 78.6g, and others 6.4g (total of 100.00g). When the specimens are not so big, we can easily get 3-5 micron-thick sections like paraffin sections with complete non-contractibility. We believe that this new methanol-cellulose nitrate medium will be useful especially for histological morphometrical analysis

Light and Electron Microscopic Studies of Microcalcifications Appearing in Monomorphic Adenomas

Microcalcifications appearing in two cases of monomorphic adenomas were studied histopathologically, electron microscopically, and electron-microanalytically. One case was basal cell adenoma that occurred in a 56-year-old man and the other was canalicular adenoma in a 71-year-old woman. The calcified granules were observed both in the lumina formed by the tumor cells and in the stromal tissues. The surroundings of the granules were stained by alcian blue and showed a sulfur peak by EPMA. These facts suggest that the surroundings contain sulfated glycosaminoglycans and that sulfur has a significant role in the mechanisms of pathological calcification as well as physiological calcification

Amyloid Deposition in Human Pulp

A tooth with a pulp having a rare degenerative change was found among numerous extracted tooth specimens. Microscopically eosinophilic dimunutive granules existed around vesseles in the coronal pulp. These were stained positively with Congo red and periodic acid-Schiff. Electron microscopic observation revealed that granules were composed of bundles showing one of the characteristic appearances of amyloid deposition

Ultrastructural Study of Varied Calcified Materials in the Pleomorphic Adenoma Occurring in the Soft Palate

A case of pleomorphic adenoma derived from the palatine gland was studied with light microscope, transmission electron microscope and X-ray microprobe analysor. Some irregular basophlic masses were found under the light microscope in the stromal tissue close to tumor cells, which were ultrastructurally composed of needle-shaped crystals and bordered by a lamina limitans-like structure. Furthermore, transmission electron microscopic findings showed that numerous membranous vesicles with appatite deposited in various degrees were scattered between collagen fibers. These vesicles seemed to be matrix vesicles which may be related to a stromal change of pleomorphic adenoma

Analytical Electron Microscopic Study of Mineral Deposits in a Case of Calcionsis Universalis

Calcinosis universalis with dermatomyositis occurred in a 58-year-old woman. Tissues removed from the patient\u27s sublingual region were studied mainly by analytical electron microscopic methods. According to an elemental analysis using a wavelength dispersive X-ray spectroscope, most of the mineral deposits contained the elements sodium, phosphorus, calcium, rhenium, and some deposits also contained sulphur and magnesium. The elements sodium, sulphur, chlorine, and calcium were found in the stromal tissues of the material. Energy dispersive X-ray spectroscopy demonstrated that the analysis-peaks of phosphorus and calcium were higher in the mineral deposits than in the stromal tissues. The mineral deposits throughout the specimen were mainly composed of hydroxyapatite, judging from field-limited area electron diffraction and X-ray diffraction examinations

術後性上顎嚢壁にみられた石灰化物の観察

Light and scanning electron microscopic observations and electron probe microanalysis were carried out on granular calcified bodies that appeared in the wall of a postoperative maxillary cyst in a 59-year-old man. The granular bodies, stained slightly with hematoxylin, were scattered in the cyst wall. They reacted positively to von Kossa\u27s stain indicating calcification. Based on scanning electron microscopic observation, secondary electron images revealed them to be compact bodies, and they appeared as light spots in composition images. Electron probe microanalysis revealed that the bodies were ccomposed mainly of calcium and phosphorus. Some of the large bodies had a membranous calcification core, which was observed especially in von Kossa\u27s stained specimens. Therefore, we believe that these calcification core must be generated cell debris, cell membrane, nuclear membrane or subcellular organelles

骨形成蛋白(BMP)誘発異所性骨形成において表示されたtranschondroid bone formation

It has been stated that BMP induces undifferentiated mesenchymal cells to become chondrocytes in the first stage of the BMP-induced heterotopic osteogenesis. The cartilage is replaced by bone in a manner similar to that in normal endochondral (indirect) ossification. It is suggested that the BMP induced-bone occurs through an endochondral-like ossification; however the cell differentiation patterns differ from those of the normal fetal endochondral ossification process. On the other hand, intramembranous (direct) ossification is observed in some cases. Therefore, we examined histopathologically the nature of the BMP induced heterotopic osteogenesis, that is, examining histological features that are more like bone than cartilage but whose cells were not distinguishable from chondrocytes. Round chondrocytes-like cells and smaller osteocytelike cells coexisted in the chondroid bone matrix. Furthermore, there were some chondroid patterns that still remained in the maturing bone matrix showing mosaic patterns. These findings seems to be a third ossification pattern, "transchondroid bone formation" , which was described by Yasui et al. (1997)11) in an experimentally distraction osteogenesis model in the rat. "Chondroid bone", a tissue intermediate between bone and cartilage, was formed mainly in BMP-inducedheterotopic osteogenesis