Stereotaxical Infusion of Rotenone: A Reliable Rodent Model for Parkinson's Disease

A clinically-related animal model of Parkinson's disease (PD) may enable the elucidation of the etiology of the disease and assist the development of medications. However, none of the current neurotoxin-based models recapitulates the main clinical features of the disease or the pathological hallmarks, such as dopamine (DA) neuron specificity of degeneration and Lewy body formation, which limits the use of these models in PD research. To overcome these limitations, we developed a rat model by stereotaxically (ST) infusing small doses of the mitochondrial complex-I inhibitor, rotenone, into two brain sites: the right ventral tegmental area and the substantia nigra. Four weeks after ST rotenone administration, tyrosine hydroxylase (TH) immunoreactivity in the infusion side decreased by 43.7%, in contrast to a 75.8% decrease observed in rats treated systemically with rotenone (SYS). The rotenone infusion also reduced the DA content, the glutathione and superoxide dismutase activities, and induced alpha-synuclein expression, when compared to the contralateral side. This ST model displays neither peripheral toxicity or mortality and has a high success rate. This rotenone-based ST model thus recapitulates the slow and specific loss of DA neurons and better mimics the clinical features of idiopathic PD, representing a reliable and more clinically-related model for PD research

Black-pigmenting gram-negative bacteria in periodontal disease. II. Screening strategies for detection of P. gingivalis.

The purpose of this analysis was to evaluate the feasibility of detecting P. gingivalis using selected sites and to indicate increased proportions of this organism in periodontitis patients. In 10 patients suffering from moderate to advanced periodontal disease, separate microbiological samples were taken from the mesial, buccal, distal and oral (lingual or palatal) aspects of every tooth. This yielded a total of 927 microbiological samples, 84 to 102 per patient. Three distinct patterns of distribution and relative proportion of P. gingivalis were recognized. In one group of patients, the organism was not cultured. In a second group, few positive sites with low proportions of P. gingivalis were present. A third group of patients yielded high frequencies and proportions of P. gingivalis. The number of samples necessary to diagnose the presence of P. gingivalis at a 95% confidence level varied considerably between the three groups. In 4 patients, sampling 4 randomly selected sites was sufficient, while in the remaining 3 positive patients, 25 or more samples were required to detect the organism with equal certainty. Seven different protocols for multiple subgingival sampling were studied. When considering the number of samples needed to detect the presence of P. gingivalis and to estimate the highest proportion of this organism, selection of the deepest pocket in each quadrant was the most efficient method of sampling.link_to_subscribed_fulltex