Interação Entre Xilanase, Glicose Oxidase E ácido Ascórbico Na Qualidade Tecnológica De Pão Elaborado Com Farinha Do Trigo Integral

This study aimed to verify the performance of xylanase and its interaction with oxidants agents (glucose oxidase and ascorbic acid) on the quality of whole wheat bread. The experiment was based on a central composite rotational design and the Response Surface Methodology was used to analyze the results. None of the xylanase, glucose oxidase or ascorbic acid concentrations within the studied range led to a significant difference in the specific volume. The highest moisture content and the lowest firmness values were reported in the bread with lower and intermediate levels of xylanase and larger amounts of glucose oxidase and ascorbic acid. This effect was observed mainly at the end of the storage period. A minimum amount of xylanase (from 33 to 63 EDX kg-1 flour) showed to be essential for obtaining best results. Levels of ascorbic acid above 63mg kg-1 and glucose oxidase above 91 SRU kg-1 proved to be necessary to offer the beneficial effect of xylanase. © 2016, Universidade Federal de Santa Maria. All rights reserved.46122249225

Relationship between childhood maltreatment and geriatric depression: the mediator effect of personality traits.

Childhood maltreatment is an important factor associated with adverse mental health outcomes including geriatric depression and the "big five" personality characteristics. The objective of this study was to evaluate a model where personality characteristics mediate the relationship between childhood maltreatment and geriatric depression. In this cross-sectional study, elderly subjects from socioeconomically disadvantaged neighborhoods of Porto Alegre, Brazil (n = 260) completed the Childhood Trauma Questionnaire (CTQ), NEO-Five Factor Inventory (NEO-FFI), and Mini International Neuropsychiatric Interview 5.0 (MINI plus). We used structural equation modeling (SEM) to evaluate the mediation hypothesis. The five personality factors (neuroticism, extraversion, agreeableness, openness, and conscientiousness) were related to childhood maltreatment and depression. Mediation analysis revealed that neuroticism and extraversion are complete mediators, agreeableness and conscientiousness are partial mediators, and openness is not a mediator. These findings support the hypothesis in which childhood maltreatment is associated with geriatric depression and mediated by personality factors. These results suggest that reducing the maladaptive personality trait in elderly people who suffered childhood maltreatment could prevent geriatric depression

Correlation Between Audiometric Data And The 35delg Mutation In Ten Patients

Mutations in the connexin 26 gene seem to be extremely common in non-syndromic hereditary deafness genesis, especially the 35delG, but there are still only a few studies that describe the audiometric characteristics of patients with these mutations. Aim: to analyze the audiometric characteristics of patients with mutations in the connexin 26 gene in order to outline genotype-phenotype correlation. Materials and Methods: Tonal audiometries of 33 index cases of non-syndromic sensorineural hearing loss were evaluated and eight affected relatives. Specific molecular tests were carried out to analyze mutations in the connexin 26 gene. Experiment Design: Retrospective, cross-sectional study. Results: A 27.3% prevalence of mutation 35delG was found in the index cases and 12.5% among the relatives affected. In relation to hearing loss degree, 41.5% of the patients were found with profound hearing loss, 39% with severe HL and 19.5% with moderate HL with homozygote and heterozygote patients for the 35delG predominating in the severe-moderate hearing losses. Conclusion: Our results suggest that the audiometric data associated with the molecular diagnose of hearing loss helped us to outline a genotype-phenotype correlation in ten patients with 35delG mutation. However, it is still necessary to run multicentric studies to verify the real phenotypic expression in the Brazilian population, as far as the 35delG mutation is concerned. © Revista Brasileira de Otorrinolaringologia. All Rights reserved.736777783Morton, N.E., Genetic epidemiology of hearing impairment (1991) Ann N Y Acad Sci, 630, pp. 16-31Mustafa, T., Arnos, K.S., Pandya, A., Advances in hereditary deafness (2001) Lancet, 358, pp. 1082-1090Skvorak Giersch, A.B., Morton, C.C., Genetic causes of nonsyndromic hearing loss (1999) Curr Opin Pediatr, 11 (6), pp. 551-557Petit, C., Genes responsible for human hereditary deafness: Symphony of a thousand (1996) Nature Genet, 14, pp. 385-391Van Camp, G., Willems, P.J., Smith, R.J.H., Nonsyndromic hearing impairment: Unparalleled heterogeneity (1997) Am J Hum Genet, 60, pp. 758-764Kelsell, D.P., Dunlop, J., Stevens, H.P., Lench, N.J., Liang, J.N., Parry, G., Mueller, R.F., Leigh, I.M., Connexin 26 mutations in hereditary non-syndromic sensorineural deafness (1997) Nature, 387, pp. 80-83Kelley, P.M., Harris, D.J., Comer, B.C., Askew, J.W., Fowler, T., Smith, S.D., Kimberling, W.J., Novel mutations in the connexin 26 gene (GJB2) that cause autossomal recessive (DFNB1) hearing loss (1998) Am J Hum Genet, 62, pp. 792-799Scott, D.A., Kraft, M.L., Carmi, R., Ramesh, A., Elbedour, K., Yari, Y., Srisailapathy, C.R.S., Identification of mutation on the connexin 26 gene that cause autossomal recessive nonsyndromic hearing loss (1998) Hum Mutat, 11, pp. 387-394Gabriel H, Kupsch P, Sudendey Jr, Winterhager E, Jahnke K, et al. Mutations in the connexin 26/GJB2 gene are the most common event in non-syndromic hearing loss among the German population. Hum Mutat 2001;17:521-2Van Camp G, Smith RJH. Na Hereditary Hearing Loss Homepage [Site na Internet]. Disponível em: http://webhost.ua.ac.be/hhh/. Acessado em 2006Zelante, L., Gasparini, P., Estivill, X., Melchionda, S., D'Agruma, L., Govea, N., Mila, M., Della Monica, M., Connexin 26 mutations associated with the most common form of non-syndromic neurosensory autossomal recessive deafness (DFNB1) in Mediterraneans (1997) Hum Molec Genet, 6, pp. 1605-1609Estivill, X., Fortina, P., Surrey, S., Rabionet, R., Melchionda, S., D'Agruma, L., Mansfield, E., Rappaport, E., Connexin 26 mutations in sporadic and inherited sensorineural deafness (1998) Lancet, 351, pp. 394-398Antoniadi, T., Gronskov, K., Sand, A., Pampanos, A., Brondum-Nielsen, K., Petersen, M.B., Mutation analysis of the GJB2 (connexin 26) gene by DGGE in greek patients with sensorineural deafness (2000) Hum Mutat, 16, pp. 7-12Oliveira, C.A., Maciel-Guerra, A.T., Sartorato, E.L., Deafness resulting from mutations in the GJB2 (connexin 26) gene on Brazilian patients (2002) Clin Genet, 61, pp. 354-358Kammen-Jolly, K., Ichiki, H., Scholtz, A.W., Gsenger, M., Kreczy, A., Schrott-Fischer, A., Connexin 26 in human fetal development of the inner ear (2001) Hear Res, 160 (1-2), pp. 15-21Denoyelle, F., Marlin, S., Weil, D., Moatti, L., Chauvin, P., Garabedian, E.N., Petit, C., Clinical features of the prevalent form of childhood deafness, DFNB1, due to a connexin 26 gene defect: Implications for genetic counselling (1999) Lancet, 17 (9161), pp. 1298-1303Cryns, K., Orzan, E., Murgia, A., Huygen, P.L.M., Moreno, F., del Castilo, I., A genotype-phenotype correlation for GJB2 (connexin 26) deafness) (2004) J Med Genet, 41, pp. 147-154(1991) Report of the informal working group on prevention of deafness and hearing impairment programme planning, , World Health Organization, Geneva: WHO, 22pAntoniadi, T., Gronskov, K., Sand, A., Pampanos, A., Brondum-Nielsen, K., Petersen, M.B., Mutation analysis of the GJB2 (connexin 26) gene by DGGE in Greek patients with sensorineural deafness (2000) Hum Mutat, 16, pp. 7-12del Castillo, I., Villamar, M., Moreno-Pelayo, M.A., del Castillo, F.J., Alvarez, A., Telleria, D., A deletion involving the connexin 30 gene in nonsyndromic hearing impairment (2002) N Engl J Med, 346, pp. 243-249Kelley, P.M., Harris, D.J., Comer, B.C., Askew, J.W., Fowler, T., Smith, S.D., Kimberling, W.J., Novel mutations in the connexin 26 gene (GJB2) that cause autossomal recessive (DFNB1) hearing loss (1998) Am J Hum Genet, 62, pp. 792-799Sobe, T., Vreugde, S., Shahin, H., Berlin, M., Davis, N., The prevalence and expression of inherited connexin 26 mutations associated with non-syndromic hearing loss in the Israeli population (2000) Hum Genet, 106, pp. 50-57Wilcox, S.A., Saunders, K., Osborn, A.H., Arnold, A., Wunderlich, J., High frequency hearing loss correlated with mutations in the GJB2 gene (2000) Hum Genet, 106, pp. 399-405del Castillo, I., Villamar, M., Moreno-Pelayo, M.A., del Castillo, F.J., Alvarez, A., Telleria, D., A deletion involving the connexin 30 gene in nonsyndromic hearing impairment (2002) N Engl J Med, 346, pp. 243-249Frei, K., Szuhai, K., Lucas, T., Weipoltshammer, K., Schofer, C., Ramsebner, R., Connexin 26 mutations in cases of sensorineural deafness in eastern Austria (2002) Eur J Hum Genet, 10, pp. 427-432Pampanos, A., Economides, J., Iliadou, V., Neou, P., Leotsakos, P., Voyiatzis, Prevalence of GJB2 mutations in prelingual deafness in the Greek population (2002) Int J Pediatr Otorhinolaryngol, 65, pp. 101-108Gasparini, P., Estivill, X., Volpini, V., Totaro, A., Castellvi-Bel, S., Linkage of DFNB1 to non-syndromic neurosensory autosomal-recessive deafness in Mediterranean families (1997) Eur J Hum Genet, 5, pp. 83-88Estivill, X., Fortina, P., Surrey, S., Rabionet, R., Melchionda, S., D'Agruma, L., Mansfield, E., Rappaport, E., Connexin 26 mutations in sporadic and inherited sensorineural deafness (1998) Lancet, 351, pp. 394-398Kenna, M.A., Wu, B.-L., Cotanche, D.A., Korf, B.R., Rehm, H.L., Connexin 26 studies in patientes with sensorineural hearing loss (2001) Arch Otolaryngol Head Neck Surg, 127, pp. 1037-1042Simsek, M., Al-Wardy, N., Al-Khayat, A., Shanmugakonar, M., Al-Bulushi, T., Al-Khabory, M., Absence of deafness associated connexin 26 (GJB2) gene mutations in the Omani population (2001) Hum Mutat, 18, pp. 545-546Nance, W.E., The genetics of deafness (2003) Ment Retard Disabil Res Rev, 9, pp. 109-119del Castillo, I., Moreno-Pelayo, M.A., del Castillo, F.J., Brownstein, Z., Marlin, S., Adina, Q., Prevalence and Evolutionary Origins of the del(GJB6-D13S1830) Mutation in the DFNB1 Locus in Hearing Impaired Subjects: A Multicenter Study (2003) Am J Hum Genet, 73, pp. 1452-1458Piatto, V.B., Oliveira, C.A., Alexandrino, F., Pimpinati, C.J., Sartorato, E.L., Perspectivas para triagem auditiva genética: Rastreamento da mutação 35delG em neonatos. (2005) J Pediatr, 81, pp. 139-142Sartorato, E.L., Gottardi, E., Oliveira, C.A., Magna, L.A., Annichio-Bizzacchi, J.M., Seixas, C.A., Maciel-Guerra, A.T., Determination of the frequency of the 35delG in Brazilian neonates (2000) Clin Genet, 58, pp. 339-340Oliveira, C.A., Alexandrino, F., Abe-Sandes, K., Silva Jr, W.A., Maciel-Guerra, A.T., Magna, L.A., Sartorato, E.L., Frequency of 35delG in the GJB2 gene in samples of Caucasians, Asians and African Brazilians (2004) Hum Biol, 76, pp. 313-316Pandya, A., Arnos, K.S., Xia, X.J., Welch, K.O., Blanton, S.H., Friedman, T.B., Frequency and distribution of GJB2 (connexin 26) and GJB6 (connexin 30) mutations in a large North American repository of deaf probands (2003) Genet Med, 5, pp. 295-303Stevenson, V.A., Ito, M., Milunsky, J.M., Connexin-30 deletion analysis in connexin-26 heterozygotes (2003) Genet Test, 7, pp. 151-154Cohn, E.S., Kelley, P.M., Fowler, T.W., Gorga, M.P., Lefkowitz, Clinical studies of families with hearing loss attributable to mutations in the connexin 26 gene (GJB2/DFNB1) (1999) Pediatrics, 103, pp. 546-550Murgia, A., Orzan, E., Polli, R., Martella, M., Vinazi, C., Leonardi, E., Arslan, E., Zacchello, F., Cx26 deafness: Mutation analysis and clinical variability (1999) J Med Genet, 36, pp. 829-832Marlin, S., Garabedian, E.-N., Roger, G., Moatti, L., Matha, N., Lewin, P., Petit, C., Denoyelle, F., Connexin 26 gene mutations in congenitally deaf children (2001) Arch Otolaryngol Head Neck Surg, 127, pp. 927-933Rabionet, R., Zelante, L., Lopez-Bigas, N., DAgruma, L., Melchionda, S., Restagno, G., Molecular basis of childhood deafness resulting from mutations in the GJB2 (connexin 26) gene (2000) Hum Genet, 106, pp. 40-44Cohn, E.S., Kelley, P.M., Clinical phenotype and mutations in connexin 26 (DFNB1/GJB2), the most commom cause of childhood hearing loss (1999) Am J Med Genet, 89, pp. 130-136Denoyelle, F., Marlin, S., Weil, D., Moatti, L., Chauvin, P., Garabedian, E.N., Petit, C., Clinical features of the prevalent form of childhood deafness, DFNB1, due to a connexin 26 gene defect: Implications for genetic counselling (1999) Lancet, 17, pp. 1298-1303Engel-Yeger, B., Zaaroura, S., Zlotogora, J., Shalev, S., Hujeirat, Y., Carrasquilo, M., Barges, S., Pratt, H., The effects of a connexin 26 mutation - 35delG - an oto-acoustic emissions and brainstem evoked potentials: Homozygotes and carriers (2002) Hear Res, 163, pp. 93-100Mustapha, M., Salem, N., Delague, V., Chouery, E., Ghassibeh, M., Rai, M., Loiselet, J., Megarbane, A., Autosomal recessive non-syndromic hearing loss in the Libanese population: Prevalence of the 30delG mutation and report of two novel mutations in the connexin 26 (GJB2) gene (2001) J Med Genet, 38, pp. e36Yoshinaga-Itano, C., Sedey, A.L., Coulter, D.K., Mehl, A.L., Language of early-and later-identified children with hearing loss (1998) Pediatrics, 102, pp. 1161-117

Moscas-das-frutas (Diptera: Tephritidae) em um pomar de goiabeira, no semiárido brasileiro

As moscas-das-frutas (Diptera: Tephritidae) são pragas-chave na cultura da goiabeira Psidium guajava L., com predominância de diferentes espécies de acordo com a região produtora no Brasil. Os objetivos do presente trabalho foram conhecer a diversidade e analisar parâmetros faunísticos das moscas-das-frutas obtidas em um pomar de goiabeira, no município de Cruzeta, Rio Grande do Norte, situado no semiárido brasileiro. As moscas-das-frutas foram coletadas semanalmente, com auxílio de armadilhas McPhail, tendo como atrativo proteína hidrolisada a 5% v/v. Foram registradas cinco espécies no pomar estudado: Ceratitis capitata (Wied.), Anastrepha zenildae Zucchi, Anastrepha sororcula Zucchi, Anastrepha obliqua (Macquart) e Anastrepha dissimilis Stone. Ceratitis capitata foi a espécie mais frequente, constante e dominante, considerada como uma praga invasiva, potencial em pomares de goiabeira no semiárido brasileiro

Hydrogen-Helium Mixtures at High Pressure

The properties of hydrogen-helium mixtures at high pressure are crucial to address important questions about the interior of Giant planets e.g. whether Jupiter has a rocky core and did it emerge via core accretion? Using path integral Monte Carlo simulations, we study the properties of these mixtures as a function of temperature, density and composition. The equation of state is calculated and compared to chemical models. We probe the accuracy of the ideal mixing approximation commonly used in such models. Finally, we discuss the structure of the liquid in terms of pair correlation functions.Comment: Proceedings article of the 5th Conference on Cryocrystals and Quantum Crystals in Wroclaw, Poland, submitted to J. Low. Temp. Phys. (2004